##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088173_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3174977
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.063768020996687	31.0	31.0	33.0	28.0	34.0
2	31.206809057199468	31.0	31.0	34.0	28.0	34.0
3	31.265267748396287	31.0	31.0	34.0	28.0	34.0
4	35.23089458600803	37.0	35.0	37.0	33.0	37.0
5	35.102558538219334	37.0	35.0	37.0	32.0	37.0
6	35.15585624714762	37.0	35.0	37.0	32.0	37.0
7	34.99725572815173	37.0	35.0	37.0	32.0	37.0
8	35.06217556851593	37.0	35.0	37.0	32.0	37.0
9	36.55831522559061	39.0	35.0	39.0	32.0	39.0
10	36.39800004850429	38.0	35.0	39.0	32.0	39.0
11	36.420912340467346	38.0	35.0	39.0	32.0	39.0
12	36.262714659035325	38.0	35.0	39.0	32.0	39.0
13	36.31167091919091	38.0	35.0	39.0	32.0	39.0
14	37.24096426525295	39.0	36.0	41.0	32.0	41.0
15	37.298626415246474	39.0	36.0	41.0	32.0	41.0
16	37.27611318129234	39.0	36.0	41.0	32.0	41.0
17	37.37054504646805	39.0	36.0	41.0	32.0	41.0
18	37.38082039649421	39.0	36.0	41.0	32.0	41.0
19	37.47204278960131	39.0	36.0	41.0	32.0	41.0
20	37.43462677052464	39.0	36.0	41.0	32.0	41.0
21	37.31855600843723	39.0	36.0	41.0	31.0	41.0
22	37.28052581168305	39.0	36.0	41.0	31.0	41.0
23	37.16630545670095	39.0	36.0	40.0	31.0	41.0
24	37.094792182746524	39.0	36.0	40.0	31.0	41.0
25	37.00827250087166	39.0	36.0	40.0	31.0	41.0
26	36.74392066462214	39.0	35.0	40.0	30.0	41.0
27	36.592199880503074	38.0	35.0	40.0	30.0	41.0
28	36.44256541071006	38.0	35.0	40.0	30.0	41.0
29	36.30153446780874	38.0	35.0	40.0	30.0	41.0
30	36.132007885411454	38.0	35.0	40.0	30.0	41.0
31	35.874438145536175	38.0	34.0	40.0	29.0	41.0
32	35.604650994322164	38.0	34.0	40.0	28.0	41.0
33	35.755690198700655	38.0	34.0	40.0	29.0	41.0
34	35.73249884959797	38.0	34.0	40.0	29.0	41.0
35	35.660599116151076	38.0	34.0	40.0	28.0	41.0
36	35.565342363110034	38.0	34.0	40.0	27.0	41.0
37	35.428437119387006	38.0	34.0	40.0	27.0	41.0
38	35.30492472858859	38.0	34.0	40.0	26.0	41.0
39	35.24538193505024	38.0	34.0	40.0	25.0	41.0
40	35.08737386129096	38.0	34.0	40.0	25.0	41.0
41	34.95074106048642	38.0	34.0	40.0	24.0	41.0
42	34.73053915036235	38.0	33.0	40.0	23.0	41.0
43	34.41651955274007	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	3.0
15	10.0
16	29.0
17	100.0
18	224.0
19	524.0
20	1103.0
21	2303.0
22	4081.0
23	7060.0
24	11770.0
25	18592.0
26	28607.0
27	41654.0
28	58087.0
29	78180.0
30	100225.0
31	122905.0
32	150165.0
33	182665.0
34	221320.0
35	267564.0
36	336813.0
37	441790.0
38	555861.0
39	543339.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.60120750481027	22.90633916403174	14.148700919723197	22.343752411434792
2	17.335369673544093	23.321964222103027	36.77730578835689	22.56536031599599
3	19.690189881690483	26.47338862612233	32.491920413911664	21.344501078275528
4	12.777068936247412	16.00184820236493	35.9113782556535	35.30970460573415
5	11.024961755628466	40.78599624501217	34.94053657711536	13.248505422244003
6	31.133831835632197	36.68621851433884	15.620963553436765	16.558986096592196
7	24.42184620550007	35.48444602905785	21.65754271605747	18.43616504938461
8	27.634121444029358	33.77539427844674	20.30899751399774	18.28148676352616
9	25.23262373239239	13.714209583250526	23.906629874799094	37.146536809557986
10	16.192337771265745	27.87270584952269	34.0119629213062	21.922993457905367
11	31.42381818828924	22.01845871639385	25.429066100321357	21.128656994995552
12	20.194539991943252	27.540010526060506	32.06728741657026	20.198162065425986
13	30.957515597750785	21.008939592318306	29.00868258258249	19.024862227348418
14	20.862135379248418	22.55896656889168	28.93828837185277	27.640609680007135
15	22.755282951656028	29.251109535596637	27.141739924415198	20.851867588332134
16	21.623684203066666	28.48855283046145	27.464419427290338	22.423343539181545
17	19.283698748053922	27.415505687127812	28.26234646739173	25.038449097426536
18	20.28720208051901	26.607720307895143	32.71620550322097	20.38887210836488
19	19.622756322329263	25.032149839195682	34.1807830418929	21.164310796582146
20	23.97280358251414	23.896236098718195	32.81526133890104	19.315698979866625
21	23.309050742729788	24.911802510695352	31.581992562465803	20.19715418410905
22	21.657479723475163	24.561248790148717	32.41393559701377	21.367335889362348
23	20.760370862529083	26.402301496987224	33.4634550108552	19.373872629628497
24	19.324014000731342	25.677823807857507	31.878719121429853	23.119443069981294
25	20.597881496464385	26.544507251548595	33.09428698223641	19.763324269750616
26	21.928788775477745	25.605697301114304	31.66388291946682	20.801631003941132
27	20.47246326508822	27.13641705121013	31.279439189638225	21.11168049406342
28	20.409281705032825	26.47530989988274	31.418967759451487	21.696440635632953
29	19.466818184824646	26.44645929718546	32.33821851307899	21.748504004910902
30	18.831412007079106	26.768067926161354	34.19760206136927	20.202918005390273
31	20.321910993370977	26.160409980922694	33.00373514516798	20.513943880538346
32	21.38846990072684	26.26403277882013	31.024886164529697	21.322611155923337
33	22.265767594536907	26.28595419746348	30.557512700091998	20.890765507907616
34	20.540495253981366	28.925910329429154	30.570174209136002	19.96342020745347
35	19.191918555630483	29.856468251581035	30.858585747235335	20.09302744555315
36	20.2659420839899	26.303340150180617	32.84458438596563	20.586133379863856
37	20.251170323438565	26.599468279612736	32.02117684632046	21.12818455062824
38	20.525314041645025	26.182772347642203	32.575511570635	20.71640204007777
39	21.420722102868776	25.795934899685886	32.44313265891375	20.340210338531588
40	19.68140241645845	26.577830327589773	31.60892189140268	22.131845364549097
41	20.663834730141353	26.05892893082375	31.506055004492943	21.77118133454195
42	18.25210072387926	27.810185711581532	31.848230711592556	22.089482852946652
43	18.92127092574214	27.468608434013852	31.484102089558448	22.126018550685565
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	351.0
2	464.0
3	1395.0
4	2326.0
5	2326.0
6	4045.0
7	5764.0
8	6289.0
9	6814.0
10	10276.5
11	13739.0
12	13739.0
13	24874.0
14	36009.0
15	59487.0
16	82965.0
17	81413.5
18	79862.0
19	79862.0
20	84543.0
21	89224.0
22	73084.5
23	56945.0
24	62549.0
25	68153.0
26	68153.0
27	74880.0
28	81607.0
29	86415.5
30	91224.0
31	98552.0
32	105880.0
33	105880.0
34	115407.5
35	124935.0
36	134542.0
37	144149.0
38	149481.0
39	154813.0
40	154813.0
41	163844.5
42	172876.0
43	193799.0
44	214722.0
45	265166.0
46	315610.0
47	315610.0
48	318274.5
49	320939.0
50	291014.5
51	261090.0
52	241323.5
53	221557.0
54	221557.0
55	192401.0
56	163245.0
57	133158.5
58	103072.0
59	89895.0
60	76718.0
61	76718.0
62	67233.5
63	57749.0
64	50307.5
65	42866.0
66	35882.0
67	28898.0
68	28898.0
69	24324.5
70	19751.0
71	16499.5
72	13248.0
73	10505.5
74	7763.0
75	7763.0
76	6075.0
77	4387.0
78	3410.0
79	2433.0
80	1876.0
81	1319.0
82	1319.0
83	1006.0
84	693.0
85	556.0
86	419.0
87	349.0
88	279.0
89	279.0
90	228.5
91	178.0
92	108.5
93	39.0
94	26.0
95	13.0
96	13.0
97	7.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3174977.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.37215650150196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.56356766392592	26.371126940405627
2	14.321868943891038	10.704782551301932
3	5.8215659529783235	6.52693421635564
4	2.926239871662315	4.374395777787962
5	1.6357257555406506	3.0565299464801368
6	1.0229685837727107	2.2938325205324137
7	0.6776743747039379	1.7728306951945136
8	0.46616849218071976	1.3937377476677641
9	0.3382992362980894	1.137867480294371
>10	1.8957388183009263	13.338703426306889
>50	0.19472170587810525	5.0889058271461725
>100	0.11775932885016761	8.0381979735956
>500	0.009062373712042217	2.269285615410672
>1k	0.0071990819207647175	5.571958080086492
>5k	6.775606513660911E-4	1.8754350712536343
>10k+	7.622557327868526E-4	6.185476130180316
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40524	1.2763557027342245	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40314	1.269741481591835	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35463	1.1169529732026406	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	21147	0.6660520690386104	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13659	0.4302078408756977	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12024	0.3787114048385232	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10969	0.3454828176708052	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10911	0.34365603278385953	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10408	0.32781339833327927	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9093	0.2863957754654601	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8447	0.26604917137982415	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7976	0.25121441824617946	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7919	0.24941912965038804	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6638	0.2090723806818128	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6565	0.20677315142755365	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6439	0.20280461874212	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6174	0.1944581015862477	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4916	0.1548357673142199	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4681	0.14743413889297466	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4606	0.14507191705640704	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4152	0.13077260087238426	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4109	0.12941826035275217	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4043	0.12733950513657263	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3832	0.12069378770302903	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3733	0.11757565487875975	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3486	0.10979607096366369	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3266	0.10286688690973195	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.149629115423513E-5	0.0
3	0.0	0.0	0.0	6.299258230847026E-5	0.0
4	0.0	0.0	0.0	9.44888734627054E-5	0.0
5	0.0	0.0	0.0	9.44888734627054E-5	0.0
6	0.0	0.0	0.0	9.44888734627054E-5	0.0
7	0.0	0.0	0.0	1.2598516461694053E-4	0.0
8	0.0	0.0	0.0	1.2598516461694053E-4	0.0
9	0.0	0.0	0.0	2.5197032923388106E-4	0.0
10	0.0	0.0	0.0	5.039406584677621E-4	0.0
11	0.0	0.0	0.0	6.92918405393173E-4	0.0
12	0.0	0.0	0.0	0.0010708738992439946	0.0
13	0.0	0.0	0.0	0.0011338664815524649	0.0
14	0.0	0.0	0.0	0.0013543405196321107	0.0
15	0.0	0.0	0.0	0.002141747798487989	0.0
16	0.0	0.0	0.0	0.002771673621572692	0.0
17	0.0	0.0	0.0	0.004251999305821743	0.0
18	0.0	0.0	0.0	0.005070902875831856	0.0
19	0.0	0.0	0.0	0.005700828698916559	0.0
20	0.0	0.0	0.0	0.007307139547782551	0.0
21	0.0	0.0	0.0	0.010078813169355242	0.0
22	0.0	0.0	0.0	0.017007997223286973	0.0
23	0.0	0.0	0.0	0.027433269595338802	0.0
24	0.0	0.0	0.0	0.04116565253858532	0.0
25	0.0	0.0	0.0	0.04645702945249682	0.0
26	0.0	0.0	0.0	0.054677561443752194	0.0
27	0.0	0.0	0.0	0.11099293002752461	0.0
28	0.0	0.0	0.0	0.17590678609640323	0.0
29	0.0	0.0	0.0	0.25367112895620975	0.0
30	0.0	0.0	0.0	0.40967225904313637	0.0
31	0.0	0.0	0.0	0.6459889315733626	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9470	0.0	29.01003	1
GTATTGG	1160	0.0	23.76293	1
ATTGGAC	1130	0.0	23.411505	3
TTGGACC	1805	0.0	20.806093	4
TATTGGA	1330	0.0	20.169172	2
GGACCCT	1835	0.0	20.062672	6
TGGACCC	1925	0.0	19.124676	5
CGTCTGT	830	0.0	18.5	34
CGTCGTA	85	2.7255646E-5	17.411764	10
GTATCAA	16585	0.0	16.64275	2
GACCCTC	2100	0.0	16.561905	7
ACCCTCG	2240	0.0	15.857143	8
CAATGCG	140	3.479181E-8	15.857143	19
TATACCG	235	0.0	15.744681	5
ACGTTTA	205	5.456968E-12	15.341463	26
TATACTG	425	0.0	15.235294	5
GAACCGT	75	0.0041065253	14.8	6
GTATACG	200	6.184564E-11	14.799999	1
CCCTCGC	2315	0.0	14.784018	9
TAGTGCG	115	2.2121594E-5	14.478261	7
>>END_MODULE