FastQCFastQC Report
Wed 25 May 2016
SRR2088172_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088172_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2500199
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT354381.4174071743889187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT348641.394449001859452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314031.256020020806344No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG188460.7537799991120707No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117660.470602540037813No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA109100.4363652653248801No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA101450.40576770089100905No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT98030.3920887897323373No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT98020.39204879291608385No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA86690.34673240010095197No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT78270.31305508081556704No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT74510.2980162779042788No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG71720.2868571661695729No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60320.24126079564066702No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59240.236941139485297No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA57820.23126159157731047No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT55430.22170235249274156No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA44400.17758586416521244No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.17242627486852047No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC42680.17070641176962315No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38090.15234787310930048No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35810.14322859900351934No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35750.14298861810599875No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG35710.14282863084098507No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34560.13822899697184104No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29020.11607076076744292No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC27990.11195108869334001No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27710.11083117783824409No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA25960.10383173499389448No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA25850.10339177001510681No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA25790.10315178911758623No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC25270.10107195467240807No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC25140.10055199606111354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA81650.029.5909371
GACGTCG358.872325E-426.4285721
GTATTGG9150.024.6666681
ATTGGAC9900.022.797983
TATTGGA11400.019.7982442
TTGGACC16850.019.652824
GGACCCT17550.019.0797736
CGTTCCG500.007037138618.524
CGCCGTT801.617909E-518.525
TGGACCC18400.018.198375
ACCGACC2600.017.0769238
GTATCAA144200.016.8321782
CGTCTGT6200.016.70967734
GACCCTC19450.016.4550137
TACCCCG1251.6598824E-716.2799995
TTTTTAC17450.016.0085951
TATACCG2200.015.9772735
CCGTTAT957.064982E-515.5789489
TCACGTT1557.219569E-915.51612924
TGCGACG855.3672824E-415.23529422
Produced by FastQC (version 0.11.5)