##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088172_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2500199
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.997361809999923	31.0	31.0	33.0	28.0	34.0
2	31.12960848316474	31.0	31.0	34.0	28.0	34.0
3	31.183684978675696	31.0	31.0	34.0	28.0	34.0
4	35.16242507096435	35.0	35.0	37.0	33.0	37.0
5	35.03280778850004	35.0	35.0	37.0	32.0	37.0
6	35.093626547326835	37.0	35.0	37.0	32.0	37.0
7	34.92041913463688	36.0	35.0	37.0	32.0	37.0
8	34.989482437198	37.0	35.0	37.0	32.0	37.0
9	36.4507301218823	38.0	35.0	39.0	32.0	39.0
10	36.30415458929469	38.0	35.0	39.0	32.0	39.0
11	36.34033010972327	38.0	35.0	39.0	32.0	39.0
12	36.166076780288286	38.0	35.0	39.0	31.0	39.0
13	36.219313742626085	38.0	35.0	39.0	32.0	39.0
14	37.106395130947575	39.0	36.0	41.0	31.0	41.0
15	37.16689191540353	39.0	36.0	41.0	31.0	41.0
16	37.14611836897783	39.0	36.0	40.0	31.0	41.0
17	37.259450147768234	39.0	36.0	40.0	32.0	41.0
18	37.26842623327183	39.0	36.0	40.0	32.0	41.0
19	37.375060145212444	39.0	36.0	41.0	32.0	41.0
20	37.34146121968691	39.0	36.0	41.0	31.0	41.0
21	37.22409856175448	39.0	36.0	41.0	31.0	41.0
22	37.175886399442604	39.0	36.0	40.0	31.0	41.0
23	37.05052997781377	39.0	36.0	40.0	31.0	41.0
24	36.98017357818318	39.0	36.0	40.0	31.0	41.0
25	36.88800971442673	39.0	35.0	40.0	31.0	41.0
26	36.610229425737714	38.0	35.0	40.0	30.0	41.0
27	36.44589530673358	38.0	35.0	40.0	30.0	41.0
28	36.29114962448989	38.0	35.0	40.0	30.0	41.0
29	36.14432291189621	38.0	35.0	40.0	30.0	41.0
30	35.95693862768523	38.0	35.0	40.0	29.0	41.0
31	35.67376276848363	38.0	34.0	40.0	29.0	41.0
32	35.39990096788296	38.0	34.0	40.0	27.0	41.0
33	35.53230682837646	38.0	34.0	40.0	28.0	41.0
34	35.49685485035391	38.0	34.0	40.0	27.0	41.0
35	35.39919222429894	38.0	34.0	40.0	27.0	41.0
36	35.2961360275722	38.0	34.0	40.0	26.0	41.0
37	35.14383015111997	38.0	34.0	40.0	26.0	41.0
38	35.0142764635935	38.0	34.0	40.0	25.0	41.0
39	34.92739417942332	38.0	33.0	40.0	25.0	41.0
40	34.75222492289614	38.0	33.0	40.0	24.0	41.0
41	34.59679729493532	38.0	33.0	40.0	23.0	41.0
42	34.35495334571368	38.0	33.0	40.0	22.0	41.0
43	34.028114962049024	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	6.0
15	9.0
16	20.0
17	77.0
18	207.0
19	476.0
20	999.0
21	2021.0
22	3589.0
23	6276.0
24	10503.0
25	15847.0
26	24568.0
27	35402.0
28	49175.0
29	65815.0
30	83477.0
31	101700.0
32	123264.0
33	149309.0
34	179845.0
35	217043.0
36	269354.0
37	349194.0
38	424071.0
39	387949.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.646244558933105	23.056444707001322	14.428971453872272	21.868339280193297
2	17.37665681811728	23.402257180328444	36.87198498999479	22.34910101155948
3	20.023286146422745	26.50237041131526	32.50461263283443	20.96973080942757
4	12.767663693969961	15.806141831110242	35.324028207354694	36.1021662675651
5	10.865855077935796	41.0862095377208	34.61368475069385	13.434250633649562
6	31.386141663123617	36.16792103348573	15.519324661756926	16.926612641633724
7	24.098361770403077	35.862065379595784	21.264707329296588	18.77486552070455
8	27.878300887249374	33.6658801959364	20.200872010587958	18.254946906226262
9	25.17003646509738	13.632674839082808	24.221791945361147	36.97549675045867
10	16.194030955135972	27.93241657964026	33.66744007177029	22.20611239345348
11	31.07556638491576	21.687953638890345	25.73983110944369	21.496648866750206
12	20.328261870355117	27.44453541498097	32.18827781308608	20.038924901577833
13	31.41465939311231	20.967251006819858	29.15095958361714	18.467130016450692
14	20.759867514545842	22.563923911656634	29.045008017361816	27.631200556435708
15	22.585962157412272	29.107923009328456	27.72007348215082	20.586041351108452
16	21.320262907072596	28.702675267048743	27.579924637998815	22.397137187879846
17	18.94997158226205	27.001130709995486	28.33666440151364	25.712233306228825
18	20.044684443118328	26.411457647971222	33.40938061330318	20.13447729560727
19	19.270466070900756	24.64167852238962	34.785111105156034	21.3027443015536
20	24.25842902904929	23.464972188213817	33.36698398807455	18.909614794662343
21	23.658236804350373	24.50088972917756	31.893621267747086	19.947252198724982
22	21.608879933157322	24.06488443519896	32.90090108827337	21.425334543370347
23	20.545884547590013	26.316705190266852	34.03161108375773	19.1057991783854
24	19.080841165043264	25.26122920615519	32.412060000024	23.245869628777548
25	20.36609885853086	26.44413504685027	33.63180290848848	19.557963186130383
26	22.17747467301603	25.07200426846023	32.01637149682885	20.73414956169489
27	20.305823656436946	26.85110265222888	31.715195470440555	21.127878220893617
28	20.113998925685518	26.26234951697845	31.737953658888753	21.885697898447283
29	19.24042846189443	26.094282895081548	32.78307046759078	21.882218175433234
30	18.480368962630575	26.35898182504673	35.12308420249748	20.03756500982522
31	20.161635133843344	25.703753981183098	33.684358725045485	20.45025215992807
32	21.442533174359323	25.91857688128025	31.367663134014535	21.271226810345897
33	22.459692208500204	25.861341437221597	30.789909123233787	20.889057231044408
34	20.2883450477342	29.153999341652405	30.93689742296513	19.620758187648264
35	18.743428023129358	30.128321785585864	31.149400507719587	19.97884968356519
36	19.957091415523323	25.907657750443065	33.62160372034386	20.51364711368975
37	20.007047439023854	26.550766558981902	32.417859538380746	21.024326463613498
38	20.504607833216475	25.924336422820744	33.023571323722635	20.54748442024015
39	21.47485060189209	25.373900237541093	32.959976385879685	20.191272774687135
40	19.392216379576187	26.350902468163532	31.928458494703822	22.32842265755646
41	20.609159510902934	25.79026709473926	31.770191092789013	21.830382301568797
42	17.89805531479694	27.648439184240935	32.26135199638109	22.192153504581036
43	18.625237431100484	27.54720724230351	31.65983987674581	22.167715449850192
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	143.0
1	245.5
2	348.0
3	1045.5
4	1743.0
5	1743.0
6	3193.0
7	4643.0
8	5201.0
9	5759.0
10	8710.5
11	11662.0
12	11662.0
13	21526.5
14	31391.0
15	52228.0
16	73065.0
17	71546.5
18	70028.0
19	70028.0
20	73077.0
21	76126.0
22	61340.0
23	46554.0
24	50857.5
25	55161.0
26	55161.0
27	59632.0
28	64103.0
29	66679.0
30	69255.0
31	73268.0
32	77281.0
33	77281.0
34	83048.0
35	88815.0
36	96258.5
37	103702.0
38	107974.0
39	112246.0
40	112246.0
41	117765.5
42	123285.0
43	142786.0
44	162287.0
45	210342.0
46	258397.0
47	258397.0
48	261358.0
49	264319.0
50	237883.0
51	211447.0
52	196108.0
53	180769.0
54	180769.0
55	155258.0
56	129747.0
57	105111.5
58	80476.0
59	69509.0
60	58542.0
61	58542.0
62	50909.5
63	43277.0
64	37759.0
65	32241.0
66	27379.0
67	22517.0
68	22517.0
69	18981.5
70	15446.0
71	13094.5
72	10743.0
73	8534.5
74	6326.0
75	6326.0
76	4976.5
77	3627.0
78	2917.5
79	2208.0
80	1714.5
81	1221.0
82	1221.0
83	924.0
84	627.0
85	471.0
86	315.0
87	261.0
88	207.0
89	207.0
90	160.0
91	113.0
92	69.5
93	26.0
94	17.0
95	8.0
96	8.0
97	5.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2500199.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.04923081033179
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.11238141813365	31.16368632154447
2	13.79689396864196	11.602975579062008
3	5.032184625177518	6.347984783528771
4	2.3624154180816292	3.9735100473920357
5	1.3041947781521603	2.7420193624074822
6	0.7345295207400683	1.8531840812760911
7	0.4870888936980304	1.4337199321380354
8	0.32282875862437205	1.0859760786887287
9	0.23436584072984512	0.8869412997816045
>10	1.38105359804421	10.91005771100983
>50	0.13341877360573565	3.9283707631213853
>100	0.08235244066278793	6.621285492611275
>500	0.007187631930072788	2.0738518158399732
>1k	0.007475137207275691	6.213616757437321
>5k	9.583509240097041E-4	2.9822475104684347
>10k+	6.708456468067928E-4	6.180572463692519
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35438	1.4174071743889187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34864	1.394449001859452	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31403	1.256020020806344	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	18846	0.7537799991120707	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11766	0.470602540037813	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10910	0.4363652653248801	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10145	0.40576770089100905	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9803	0.3920887897323373	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9802	0.39204879291608385	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8669	0.34673240010095197	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7827	0.31305508081556704	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7451	0.2980162779042788	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7172	0.2868571661695729	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6032	0.24126079564066702	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5924	0.236941139485297	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5782	0.23126159157731047	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5543	0.22170235249274156	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4440	0.17758586416521244	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4311	0.17242627486852047	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4268	0.17070641176962315	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3809	0.15234787310930048	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3581	0.14322859900351934	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3575	0.14298861810599875	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3571	0.14282863084098507	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3456	0.13822899697184104	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2902	0.11607076076744292	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2799	0.11195108869334001	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2771	0.11083117783824409	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2596	0.10383173499389448	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2585	0.10339177001510681	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2579	0.10315178911758623	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2527	0.10107195467240807	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2514	0.10055199606111354	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.999681625342623E-5	0.0	0.0	0.0	0.0
7	3.999681625342623E-5	0.0	0.0	0.0	0.0
8	3.999681625342623E-5	0.0	0.0	0.0	0.0
9	3.999681625342623E-5	0.0	0.0	0.0	0.0
10	3.999681625342623E-5	0.0	0.0	1.9998408126713113E-4	0.0
11	3.999681625342623E-5	0.0	0.0	4.399649787876885E-4	0.0
12	3.999681625342623E-5	0.0	0.0	0.0011199108550959344	0.0
13	3.999681625342623E-5	0.0	0.0	0.001239901303856213	0.0
14	3.999681625342623E-5	0.0	0.0	0.0015198790176301966	0.0
15	3.999681625342623E-5	0.0	0.0	0.001919847180164459	0.0
16	3.999681625342623E-5	0.0	0.0	0.0027197835052329834	0.0
17	3.999681625342623E-5	0.0	0.0	0.004319656155370033	0.0
18	3.999681625342623E-5	0.0	0.0	0.004919608399171426	0.0
19	3.999681625342623E-5	0.0	0.0	0.005879531989253655	0.0
20	3.999681625342623E-5	0.0	0.0	0.007759382353164688	0.0
21	3.999681625342623E-5	0.0	0.0	0.011919051243521016	0.0
22	3.999681625342623E-5	0.0	0.0	0.019998408126713114	0.0
23	3.999681625342623E-5	0.0	0.0	0.0343172683454397	0.0
24	3.999681625342623E-5	0.0	0.0	0.05379571786085827	0.0
25	3.999681625342623E-5	0.0	0.0	0.061035141602728424	0.0
26	3.999681625342623E-5	0.0	0.0	0.0690745016696671	0.0
27	3.999681625342623E-5	0.0	0.0	0.13910892692941643	0.0
28	3.999681625342623E-5	0.0	0.0	0.2110631993693302	0.0
29	3.999681625342623E-5	0.0	0.0	0.3050557175648818	0.0
30	3.999681625342623E-5	0.0	0.0	0.4962005024400058	0.0
31	3.999681625342623E-5	0.0	0.0	0.7646591331330026	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8165	0.0	29.590937	1
GACGTCG	35	8.872325E-4	26.42857	21
GTATTGG	915	0.0	24.666668	1
ATTGGAC	990	0.0	22.79798	3
TATTGGA	1140	0.0	19.798244	2
TTGGACC	1685	0.0	19.65282	4
GGACCCT	1755	0.0	19.079773	6
CGTTCCG	50	0.0070371386	18.5	24
CGCCGTT	80	1.617909E-5	18.5	25
TGGACCC	1840	0.0	18.19837	5
ACCGACC	260	0.0	17.076923	8
GTATCAA	14420	0.0	16.832178	2
CGTCTGT	620	0.0	16.709677	34
GACCCTC	1945	0.0	16.455013	7
TACCCCG	125	1.6598824E-7	16.279999	5
TTTTTAC	1745	0.0	16.008595	1
TATACCG	220	0.0	15.977273	5
CCGTTAT	95	7.064982E-5	15.578948	9
TCACGTT	155	7.219569E-9	15.516129	24
TGCGACG	85	5.3672824E-4	15.235294	22
>>END_MODULE