FastQCFastQC Report
Wed 25 May 2016
SRR2088171_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088171_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2154254
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT324241.5051149957247383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT323621.5022369692710331No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT283441.315722287158339No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG165420.7678760257611219No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105570.4900536334155582No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA95680.44414446950081093No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT89520.4155498840898056No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT89390.4149464269301577No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA87430.40584814975392874No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA76490.35506490878048735No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT69580.3229888397561291No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT67490.3132871054202522No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG65380.30349253152135264No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54430.25266287076639987No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52190.24226483970785245No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA51840.24064014735495445No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT49390.22926730088466818No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39510.18340455675143227No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA39370.18275467981027305No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC38150.17709146646588564No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32520.150957129474983No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG32200.14947169646661906No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC31520.14631515132384576No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30350.14088403688701517No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG30320.14074477754248105No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28100.1304395860469564No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24890.11553883618180587No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA23550.10931858545928197No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC23480.10899364698870236No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG22990.10671907769464511No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC22970.10662623813162238No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC22910.10634771944255414No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC22510.10449092818209922No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA22480.10435166883756511No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC22400.10398031058547413No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA21950.10189142041746237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73150.029.5645921
ATTGGAC8100.023.524693
GTATTGG8650.022.4566461
TATTGGA9650.020.3212432
ACGCATA555.1451015E-420.1818187
CCGTTAT656.905711E-519.9230779
GCGATCA801.6177397E-518.535
TTGGACC14350.018.048784
ACCGTTA852.7249058E-517.4117648
CCCGTTG752.0683244E-417.26666631
GTATCAA128650.016.9109972
GGACCCT14450.016.8996546
TGGACCC15600.016.1282065
ACGTTTA1504.6766218E-916.03333326
TCTATAC1851.8189894E-1116.03
CGCCACA1751.3278623E-1015.8571431
CGATCAC1059.350606E-615.85714236
GACCCTC16000.015.3781267
CGGGCGT855.366855E-415.2352946
TTACGCC855.366855E-415.23529429
Produced by FastQC (version 0.11.5)