##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088171_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2154254
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.109386822538102	31.0	31.0	33.0	30.0	34.0
2	31.245316011946596	31.0	31.0	34.0	28.0	34.0
3	31.281907333118564	31.0	31.0	34.0	28.0	34.0
4	35.25113380316341	37.0	35.0	37.0	33.0	37.0
5	35.14163975093002	37.0	35.0	37.0	32.0	37.0
6	35.204810574797584	37.0	35.0	37.0	32.0	37.0
7	35.032763081790726	37.0	35.0	37.0	32.0	37.0
8	35.10743672751681	37.0	35.0	37.0	32.0	37.0
9	36.58402444651374	39.0	35.0	39.0	32.0	39.0
10	36.43938412090682	38.0	35.0	39.0	32.0	39.0
11	36.47494167354453	38.0	35.0	39.0	32.0	39.0
12	36.30675723475505	38.0	35.0	39.0	32.0	39.0
13	36.35524223234586	38.0	35.0	39.0	32.0	39.0
14	37.26527326861178	39.0	36.0	41.0	32.0	41.0
15	37.32841484801699	39.0	36.0	41.0	32.0	41.0
16	37.29841884940216	39.0	36.0	41.0	32.0	41.0
17	37.43489068605652	39.0	36.0	41.0	32.0	41.0
18	37.45401656443484	39.0	36.0	41.0	32.0	41.0
19	37.55797459352518	39.0	36.0	41.0	32.0	41.0
20	37.524576953321194	39.0	36.0	41.0	32.0	41.0
21	37.40357311626206	39.0	36.0	41.0	32.0	41.0
22	37.36895602839777	39.0	36.0	41.0	32.0	41.0
23	37.24499525125635	39.0	36.0	41.0	31.0	41.0
24	37.17110331465092	39.0	36.0	41.0	31.0	41.0
25	37.08303477677191	39.0	36.0	40.0	31.0	41.0
26	36.80713230658966	39.0	35.0	40.0	31.0	41.0
27	36.6480674980759	38.0	35.0	40.0	30.0	41.0
28	36.490291766894714	38.0	35.0	40.0	30.0	41.0
29	36.34802720570555	38.0	35.0	40.0	30.0	41.0
30	36.160931812126144	38.0	35.0	40.0	30.0	41.0
31	35.87298526543295	38.0	35.0	40.0	29.0	41.0
32	35.586961890287775	38.0	34.0	40.0	28.0	41.0
33	35.70023590532964	38.0	34.0	40.0	29.0	41.0
34	35.654345773525314	38.0	34.0	40.0	29.0	41.0
35	35.55270177054331	38.0	34.0	40.0	27.0	41.0
36	35.44181837424927	38.0	34.0	40.0	27.0	41.0
37	35.294773968157884	38.0	34.0	40.0	26.0	41.0
38	35.14781451026666	38.0	34.0	40.0	25.0	41.0
39	35.05435199377604	38.0	34.0	40.0	25.0	41.0
40	34.86377279559421	38.0	34.0	40.0	24.0	41.0
41	34.70087789090794	38.0	33.0	40.0	23.0	41.0
42	34.44930820599614	38.0	33.0	40.0	22.0	41.0
43	34.119505870709766	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	4.0
15	4.0
16	13.0
17	38.0
18	135.0
19	350.0
20	748.0
21	1421.0
22	2697.0
23	4779.0
24	8107.0
25	12682.0
26	19431.0
27	28055.0
28	39886.0
29	53509.0
30	68241.0
31	83507.0
32	101723.0
33	124208.0
34	151902.0
35	182036.0
36	229062.0
37	303406.0
38	369240.0
39	369065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.68401404848267	23.17475098108208	14.460736756204238	21.680498214231005
2	17.180425335174032	23.374773819614585	37.24811466057392	22.196686184637464
3	19.812659045776403	26.514932779514393	32.77157661074321	20.900831563965994
4	12.5037716072478	15.642445134139244	35.46174220867177	36.392041049941184
5	10.634539845347856	41.309799123037486	34.563333757300676	13.492327274313986
6	30.999037253731455	36.1794384506191	15.469345768883336	17.35217852676611
7	23.86775189926536	36.16295942818256	21.269265369821756	18.70002330273032
8	27.810833819967378	33.66529666418166	20.17519753938022	18.34867197647074
9	25.050342253049084	13.697224189905183	24.60921506934651	36.64321848769922
10	16.361719648657957	28.083689295691222	33.49730347489201	22.057287580758814
11	30.641837035001444	21.919235150543994	25.865659295514824	21.573268518939734
12	20.3134820685026	27.860735085092102	32.04208974429199	19.783693102113308
13	31.36222562427643	21.140032698094096	29.46286742417561	18.034874253453864
14	20.707493173971127	22.894050562282814	29.228029749509577	27.170426514236485
15	22.56818369607298	29.21716752063591	27.913189438199954	20.301459345091153
16	21.119607994229092	28.751345013169292	27.839057047126293	22.289989945475323
17	18.64501586163934	27.330528340669208	28.47960361220172	25.544852185489734
18	19.796040763995332	26.602666166570888	33.70368582349156	19.897607245942215
19	18.926923194757908	24.74856725344365	35.34778164506135	20.97672790673709
20	24.161867634921418	23.355834548757944	33.80567008347205	18.676627732848587
21	23.39241333658891	24.512197725987743	32.29614520850374	19.799243728919617
22	21.29646736178742	24.192365431374387	33.31519867202289	21.1959685348153
23	20.222127938488217	26.517393027934492	34.50814063708365	18.752338396493634
24	18.520053809810726	25.522663529927296	32.92118756655436	23.036095093707612
25	19.990121870494377	26.642215820418574	34.13585398936244	19.2318083197246
26	21.68811105839887	25.277427824202718	32.57373550194174	20.460725615456674
27	20.036263133316684	27.028057044341104	32.097561383198084	20.83811843914413
28	19.779329642651238	26.447670516104417	32.152429564944526	21.620570276299826
29	18.767285566140295	26.332410198611676	33.219898860580045	21.680405374667984
30	17.954892969909768	26.639152114838826	35.63693046409569	19.769024451155715
31	19.72966047643407	25.95487811557969	34.14657695889157	20.168884449094676
32	21.102850453103486	26.26120225377323	31.68553940250314	20.95040789062014
33	22.213211626855514	26.1075991967521	31.085006689090516	20.594182487301868
34	20.02800041220766	29.43311234422682	31.2857722441272	19.25311499943832
35	18.422200910384756	30.427331224637395	31.47618618788685	19.674281677091003
36	19.515526024322106	26.16729503577573	34.08131074608658	20.235868193815584
37	19.57768211176584	26.749631194835892	32.83925665218679	20.833430041211482
38	20.115408860793575	26.133455015053936	33.52199879865606	20.229137325496435
39	21.215743361739143	25.5153292044485	33.37378043629024	19.89514699752211
40	19.107171206366566	26.51753228727903	32.33894424705721	22.03635225929719
41	20.308840090351463	25.911986237463175	32.165427103767705	21.613746568417653
42	17.431231414679978	27.893832389309708	32.614259971201164	22.060676224809146
43	18.187734593970813	27.768266880321445	32.125366832323394	21.918631693384345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	129.0
1	185.0
2	241.0
3	896.5
4	1552.0
5	1552.0
6	2875.5
7	4199.0
8	4624.5
9	5050.0
10	7789.5
11	10529.0
12	10529.0
13	19340.5
14	28152.0
15	47399.5
16	66647.0
17	65260.0
18	63873.0
19	63873.0
20	66592.5
21	69312.0
22	55397.5
23	41483.0
24	44839.0
25	48195.0
26	48195.0
27	52634.0
28	57073.0
29	58369.5
30	59666.0
31	62728.5
32	65791.0
33	65791.0
34	71453.0
35	77115.0
36	83912.0
37	90709.0
38	94627.0
39	98545.0
40	98545.0
41	102704.0
42	106863.0
43	124299.0
44	141735.0
45	185701.5
46	229668.0
47	229668.0
48	231922.0
49	234176.0
50	208810.5
51	183445.0
52	168493.0
53	153541.0
54	153541.0
55	130516.5
56	107492.0
57	84955.5
58	62419.0
59	53138.0
60	43857.0
61	43857.0
62	38105.5
63	32354.0
64	27863.5
65	23373.0
66	19690.0
67	16007.0
68	16007.0
69	13741.5
70	11476.0
71	9685.0
72	7894.0
73	6378.5
74	4863.0
75	4863.0
76	3961.5
77	3060.0
78	2354.0
79	1648.0
80	1331.5
81	1015.0
82	1015.0
83	776.5
84	538.0
85	418.5
86	299.0
87	231.5
88	164.0
89	164.0
90	122.0
91	80.0
92	48.0
93	16.0
94	11.5
95	7.0
96	7.0
97	4.0
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2154254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.113995750084854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.25696789825244	28.653727310363486
2	13.799395196151764	10.79498970112043
3	5.144048619543757	6.036128875291932
4	2.4590839965076614	3.847384039540094
5	1.3916594569132608	2.7216681041635336
6	0.8186073053582358	1.921140159766227
7	0.5588102172399732	1.5300110323559812
8	0.3810051967948057	1.1922108518553818
9	0.27864161976989194	0.980890841832868
>10	1.6305126210009908	12.099891929729225
>50	0.16063062181576154	4.383176464786221
>100	0.10053212066310657	7.467072787618904
>500	0.009454198030270245	2.4983391998379645
>1k	0.00873615767354086	6.503555201077081
>5k	0.0013164073206705406	3.742008736879069
>10k+	5.983669639411548E-4	5.627804763781661
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32424	1.5051149957247383	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32362	1.5022369692710331	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28344	1.315722287158339	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16542	0.7678760257611219	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10557	0.4900536334155582	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9568	0.44414446950081093	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8952	0.4155498840898056	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8939	0.4149464269301577	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8743	0.40584814975392874	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7649	0.35506490878048735	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6958	0.3229888397561291	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6749	0.3132871054202522	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6538	0.30349253152135264	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5443	0.25266287076639987	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5219	0.24226483970785245	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5184	0.24064014735495445	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4939	0.22926730088466818	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3951	0.18340455675143227	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3937	0.18275467981027305	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3815	0.17709146646588564	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3252	0.150957129474983	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3220	0.14947169646661906	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3152	0.14631515132384576	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3035	0.14088403688701517	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3032	0.14074477754248105	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2810	0.1304395860469564	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2489	0.11553883618180587	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2355	0.10931858545928197	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2348	0.10899364698870236	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2299	0.10671907769464511	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2297	0.10662623813162238	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2291	0.10634771944255414	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2251	0.10449092818209922	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2248	0.10435166883756511	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2240	0.10398031058547413	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2195	0.10189142041746237	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.6419781511372385E-5	0.0
3	0.0	0.0	0.0	4.6419781511372385E-5	0.0
4	0.0	0.0	0.0	4.6419781511372385E-5	0.0
5	0.0	0.0	0.0	4.6419781511372385E-5	0.0
6	0.0	0.0	0.0	4.6419781511372385E-5	0.0
7	0.0	0.0	0.0	4.6419781511372385E-5	0.0
8	0.0	0.0	0.0	4.6419781511372385E-5	0.0
9	0.0	0.0	0.0	1.3925934453411714E-4	0.0
10	0.0	0.0	0.0	3.713582520909791E-4	0.0
11	0.0	0.0	0.0	6.03457159647841E-4	0.0
12	0.0	0.0	0.0	0.0012997538823184267	0.0
13	0.0	0.0	0.0	0.0015318527898752887	0.0
14	0.0	0.0	0.0	0.0019032110419662676	0.0
15	0.0	0.0	0.0	0.003017285798239205	0.0
16	0.0	0.0	0.0	0.005245435310785079	0.0
17	0.0	0.0	0.0	0.007473584823330954	0.0
18	0.0	0.0	0.0	0.008448400235069774	0.0
19	0.0	0.0	0.0	0.00993383324343369	0.0
20	0.0	0.0	0.0	0.012997538823184267	0.0
21	0.0	0.0	0.0	0.018010875226412485	0.0
22	0.0	0.0	0.0	0.028780264537050876	0.0
23	0.0	0.0	0.0	0.04614126282230415	0.0
24	0.0	0.0	0.0	0.07111510527542249	0.0
25	0.0	0.0	0.0	0.07937782638444678	0.0
26	0.0	0.0	0.0	0.08921882006485772	0.0
27	0.0	0.0	0.0	0.16414034742421274	0.0
28	0.0	0.0	0.0	0.24579274310271676	0.0
29	0.0	0.0	0.0	0.3475913239571564	0.0
30	0.0	0.0	0.0	0.5566660198843776	0.0
31	0.0	0.0	0.0	0.8460933576077844	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7315	0.0	29.564592	1
ATTGGAC	810	0.0	23.52469	3
GTATTGG	865	0.0	22.456646	1
TATTGGA	965	0.0	20.321243	2
ACGCATA	55	5.1451015E-4	20.181818	7
CCGTTAT	65	6.905711E-5	19.923077	9
GCGATCA	80	1.6177397E-5	18.5	35
TTGGACC	1435	0.0	18.04878	4
ACCGTTA	85	2.7249058E-5	17.411764	8
CCCGTTG	75	2.0683244E-4	17.266666	31
GTATCAA	12865	0.0	16.910997	2
GGACCCT	1445	0.0	16.899654	6
TGGACCC	1560	0.0	16.128206	5
ACGTTTA	150	4.6766218E-9	16.033333	26
TCTATAC	185	1.8189894E-11	16.0	3
CGCCACA	175	1.3278623E-10	15.857143	1
CGATCAC	105	9.350606E-6	15.857142	36
GACCCTC	1600	0.0	15.378126	7
CGGGCGT	85	5.366855E-4	15.235294	6
TTACGCC	85	5.366855E-4	15.235294	29
>>END_MODULE