FastQCFastQC Report
Wed 25 May 2016
SRR2088170_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088170_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3463593
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT352231.0169497397644585No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT345440.9973458197888724No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT315450.9107594339173223No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG212950.6148239703683429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123930.35780762924512205No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA111360.3215158363006277No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA105130.303528734467358No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT101630.29342362107788067No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT100260.2894681909797138No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA83980.24246497784237353No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT80190.2315225836291966No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG77020.22237023807358428No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT71950.20773225953511282No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA62700.18102588843435127No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA61930.17880276348866625No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA58080.16768713876024116No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT50650.14623542662200784No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.12137684768389358No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC41430.11961567077887038No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA40070.1156891124332449No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC39530.11413003779601125No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG38810.11205127161303305No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG37390.10795148275215939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85600.028.4415871
ATTGGAC11800.022.1059323
GTATTGG11900.021.298321
TATTGGA15450.018.2006472
TTGGACC19400.017.5463924
TGGACCC19300.016.9663225
GGACCCT19800.016.7247476
GTATCAA147150.016.595312
CAATGCG1557.223207E-915.516129519
TAGTACT4950.015.3232314
TATACTG4750.015.1894735
GACCCTC21100.015.1682457
ATTCGCG750.00410662414.817
CGATCGC1651.6483682E-814.57575719
CGATCCG908.279945E-414.38888928
CTAGTAC4250.014.3647053
CCCGTAT1702.4414476E-814.1470591
GCGTTAT2750.014.1272721
ACGTTTA2500.014.05999926
TATACCG2900.014.0344835
Produced by FastQC (version 0.11.5)