##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088170_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3463593
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12613375763261	31.0	31.0	33.0	30.0	34.0
2	31.26438181391405	31.0	31.0	34.0	28.0	34.0
3	31.325671347643905	31.0	31.0	34.0	28.0	34.0
4	35.28716162666918	37.0	35.0	37.0	33.0	37.0
5	35.149778279376356	37.0	35.0	37.0	32.0	37.0
6	35.19544559652361	37.0	35.0	37.0	32.0	37.0
7	35.04695441987555	37.0	35.0	37.0	32.0	37.0
8	35.09801671270267	37.0	35.0	37.0	32.0	37.0
9	36.62235141369093	39.0	35.0	39.0	32.0	39.0
10	36.46147425520262	38.0	35.0	39.0	32.0	39.0
11	36.47666368421463	38.0	35.0	39.0	32.0	39.0
12	36.32791006333596	38.0	35.0	39.0	32.0	39.0
13	36.379361547387354	38.0	35.0	39.0	32.0	39.0
14	37.342367882138575	39.0	36.0	41.0	32.0	41.0
15	37.40049740255278	39.0	36.0	41.0	32.0	41.0
16	37.38117151755417	39.0	36.0	41.0	32.0	41.0
17	37.45011812877552	39.0	36.0	41.0	32.0	41.0
18	37.44508838076529	39.0	36.0	41.0	32.0	41.0
19	37.52595325143572	39.0	36.0	41.0	32.0	41.0
20	37.48245593520948	39.0	36.0	41.0	32.0	41.0
21	37.361648727203224	39.0	36.0	41.0	31.0	41.0
22	37.31955197969276	39.0	36.0	41.0	31.0	41.0
23	37.2305978213953	39.0	36.0	41.0	31.0	41.0
24	37.15576541470086	39.0	36.0	41.0	31.0	41.0
25	37.06792570605149	39.0	36.0	40.0	31.0	41.0
26	36.81794050282467	39.0	35.0	40.0	31.0	41.0
27	36.66559956669274	39.0	35.0	40.0	30.0	41.0
28	36.53222852685059	38.0	35.0	40.0	30.0	41.0
29	36.397228254012525	38.0	35.0	40.0	30.0	41.0
30	36.24551065901796	38.0	35.0	40.0	30.0	41.0
31	36.02594646657387	38.0	35.0	40.0	30.0	41.0
32	35.77060959529598	38.0	34.0	40.0	29.0	41.0
33	35.94422323870039	38.0	35.0	40.0	29.0	41.0
34	35.94306172809565	38.0	35.0	40.0	29.0	41.0
35	35.86275321609669	38.0	35.0	40.0	29.0	41.0
36	35.79034083970028	38.0	35.0	40.0	28.0	41.0
37	35.666490548976164	38.0	34.0	40.0	27.0	41.0
38	35.5565258389193	38.0	34.0	40.0	27.0	41.0
39	35.52847722004289	38.0	34.0	40.0	27.0	41.0
40	35.39439564637069	38.0	34.0	40.0	26.0	41.0
41	35.28364418105707	38.0	34.0	40.0	25.0	41.0
42	35.1018705142319	38.0	34.0	40.0	25.0	41.0
43	34.801497173599785	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	4.0
15	9.0
16	40.0
17	70.0
18	245.0
19	501.0
20	1087.0
21	2230.0
22	4057.0
23	7058.0
24	11962.0
25	18907.0
26	28587.0
27	42018.0
28	59283.0
29	80804.0
30	104214.0
31	129281.0
32	158957.0
33	194180.0
34	237822.0
35	289390.0
36	362853.0
37	473415.0
38	610179.0
39	646438.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.254587360581915	22.12785971099953	13.353791857184143	23.26376107123441
2	17.439780020343036	22.796327397589728	36.608400582862934	23.155491999204294
3	19.653723748719898	26.181078435023974	31.65657743274109	22.508620383515037
4	12.877754401282138	15.713855525172848	35.87690586047494	35.53148421307007
5	11.640946265915193	40.035217763749955	34.910885892193456	13.412950078141398
6	31.89736784893606	36.29003176758932	15.7716856455132	16.04091473796142
7	25.501119790922317	34.35143794319945	21.67699842331359	18.47044384256464
8	27.54705879126098	33.7568530713626	20.215741283690086	18.48034685368633
9	25.578178498455216	13.628766428388092	22.99978663774872	37.793268435407974
10	16.26729814963825	27.27627062417553	33.95038042864736	22.506050797538858
11	32.23681304356488	21.798230912234782	24.887854895191207	21.07710114900914
12	20.465770660698297	26.724098356821948	31.849065406934358	20.961065575545394
13	30.86679064197208	20.678180144145113	28.243936282351882	20.211092931530928
14	21.09035328342562	22.052388949856404	28.05826204175837	28.798995724959603
15	23.15705107384153	28.789958866414157	26.325581556493503	21.727408503250814
16	22.19787371091234	28.28242232848952	26.656538455875157	22.863165504722986
17	20.11220717907676	26.958767961478152	27.627870826624264	25.301154032820833
18	21.076292739937976	26.41571916792764	31.321549616251097	21.186438475883282
19	20.49184185324315	24.969966159418846	32.42205420787027	22.11613777946774
20	24.395360540340626	24.021096012146923	31.255808635714416	20.327734811798038
21	23.83879976660075	24.938726923169092	30.157902501823976	21.064570808406184
22	22.492827534874912	24.460235368301067	31.066409939043073	21.98052715778095
23	21.464531196361698	26.074599411651427	31.949856695056262	20.511012696930614
24	20.328196759838697	25.44063346934816	30.585493156961576	23.645676613851567
25	21.517828451553058	26.181626998322262	31.456582802887063	20.84396174723762
26	22.703446969664164	25.39957783723434	30.348513812102055	21.54846138099944
27	21.373325330083528	26.70637110076155	30.03121325167247	21.889090317482452
28	21.277326752883493	26.023178820375257	30.183973694368827	22.515520732372423
29	20.41498524797804	26.089121903179734	31.003325159740193	22.49256768910204
30	19.919112898080115	26.339613228228604	32.59245529136939	21.148818582321883
31	21.205205115035167	25.83048874391419	31.5734556571745	21.390850483876136
32	22.102712414536004	25.9097128328877	29.831160878313355	22.156413874262938
33	22.85412864617754	25.849919433374534	29.498731519552095	21.797220400895835
34	21.391514534184587	28.215093401563056	29.52104938426657	20.872342679985785
35	20.218051023893395	29.000520557698323	29.819063613998527	20.962364804409756
36	20.99781931653055	25.923340300087222	31.55232153431422	21.526518849068005
37	21.17590028620568	26.093741383586348	30.771860319616074	21.95849801059189
38	21.27596978051405	25.6795183498754	31.421994443342506	21.62251742626804
39	22.117263777816852	25.31209642703401	31.294439040614762	21.27620075453438
40	20.5598637022306	26.047864168798124	30.619330850940052	22.772941278031222
41	21.355395971755343	25.56287646960829	30.589448587059735	22.492278971576628
42	19.30931261265397	27.126252997970607	30.893439269567757	22.670995119807667
43	19.835673533235575	26.72929527227939	30.601286005601697	22.833745188883338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	196.0
1	306.5
2	417.0
3	1297.5
4	2178.0
5	2178.0
6	3810.0
7	5442.0
8	5871.0
9	6300.0
10	9521.5
11	12743.0
12	12743.0
13	22767.5
14	32792.0
15	52709.5
16	72627.0
17	72310.0
18	71993.0
19	71993.0
20	76744.0
21	81495.0
22	68320.0
23	55145.0
24	61177.5
25	67210.0
26	67210.0
27	74839.5
28	82469.0
29	88793.5
30	95118.0
31	102989.5
32	110861.0
33	110861.0
34	120991.5
35	131122.0
36	142385.5
37	153649.0
38	161477.5
39	169306.0
40	169306.0
41	177370.0
42	185434.0
43	207296.0
44	229158.0
45	279191.5
46	329225.0
47	329225.0
48	333242.0
49	337259.0
50	309806.0
51	282353.0
52	265502.5
53	248652.0
54	248652.0
55	219734.5
56	190817.0
57	162549.0
58	134281.0
59	119665.0
60	105049.0
61	105049.0
62	93249.5
63	81450.0
64	72003.0
65	62556.0
66	53394.0
67	44232.0
68	44232.0
69	37879.0
70	31526.0
71	26540.0
72	21554.0
73	17239.5
74	12925.0
75	12925.0
76	10033.5
77	7142.0
78	5516.0
79	3890.0
80	3075.0
81	2260.0
82	2260.0
83	1720.0
84	1180.0
85	965.0
86	750.0
87	613.0
88	476.0
89	476.0
90	383.0
91	290.0
92	169.0
93	48.0
94	33.0
95	18.0
96	18.0
97	11.5
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3463593.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.68312656644051
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.69534146763317	31.02864460653597
2	14.229143180630096	12.146886386216764
3	5.3453974956824215	6.844748335684406
4	2.6276695848434963	4.486286138586445
5	1.3621049446183136	2.9069448873958956
6	0.8585922068995238	2.1988439901631107
7	0.5483394840292605	1.638339051873832
8	0.38678175921311503	1.3207243825667123
9	0.27989276371853783	1.0752028432946257
>10	1.41256563001068	11.40218154792252
>50	0.1443071613397079	4.29367858394302
>100	0.09727664325014382	7.808941396985353
>500	0.006531984328176152	1.905171010966722
>1k	0.004898988245429147	4.220476549168478
>5k	5.443320272699052E-4	1.5871584716151534
>10k+	6.123735306786433E-4	5.135771817081071
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35223	1.0169497397644585	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34544	0.9973458197888724	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31545	0.9107594339173223	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	21295	0.6148239703683429	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12393	0.35780762924512205	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11136	0.3215158363006277	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10513	0.303528734467358	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10163	0.29342362107788067	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10026	0.2894681909797138	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8398	0.24246497784237353	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8019	0.2315225836291966	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7702	0.22237023807358428	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7195	0.20773225953511282	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6270	0.18102588843435127	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6193	0.17880276348866625	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5808	0.16768713876024116	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5065	0.14623542662200784	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4204	0.12137684768389358	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4143	0.11961567077887038	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4007	0.1156891124332449	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3953	0.11413003779601125	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3881	0.11205127161303305	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3739	0.10795148275215939	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.8871752541363838E-5	0.0
5	0.0	0.0	0.0	2.8871752541363838E-5	0.0
6	2.8871752541363838E-5	0.0	0.0	2.8871752541363838E-5	0.0
7	2.8871752541363838E-5	0.0	0.0	2.8871752541363838E-5	0.0
8	2.8871752541363838E-5	0.0	0.0	2.8871752541363838E-5	0.0
9	2.8871752541363838E-5	0.0	0.0	8.661525762409152E-5	0.0
10	2.8871752541363838E-5	0.0	0.0	8.661525762409152E-5	0.0
11	2.8871752541363838E-5	0.0	0.0	3.1758927795500223E-4	0.0
12	2.8871752541363838E-5	0.0	0.0	5.196915457445491E-4	0.0
13	2.8871752541363838E-5	0.0	0.0	5.196915457445491E-4	0.0
14	2.8871752541363838E-5	0.0	0.0	7.506655660754598E-4	0.0
15	2.8871752541363838E-5	0.0	0.0	0.0011259983491131898	0.0
16	2.8871752541363838E-5	0.0	0.0	0.001963279172812741	0.0
17	2.8871752541363838E-5	0.0	0.0	0.0031758927795500222	0.0
18	2.8871752541363838E-5	0.0	0.0	0.0036378408202118436	0.0
19	2.8871752541363838E-5	0.0	0.0	0.004446249891370031	0.0
20	2.8871752541363838E-5	0.0	0.0	0.005658863498107312	0.0
21	2.8871752541363838E-5	0.0	0.0	0.008401679989536876	0.0
22	2.8871752541363838E-5	0.0	0.0	0.014349261013057828	0.0
23	2.8871752541363838E-5	0.0	0.0	0.025002937700821085	0.0
24	2.8871752541363838E-5	0.0	0.0	0.03891912242575846	0.0
25	2.8871752541363838E-5	0.0	0.0	0.045328651489941224	0.0
26	2.8871752541363838E-5	0.0	0.0	0.05329725519135765	0.0
27	2.8871752541363838E-5	0.0	0.0	0.11300403944689806	0.0
28	2.8871752541363838E-5	0.0	0.0	0.17487620514304078	0.0
29	2.8871752541363838E-5	0.0	0.0	0.2532341415403022	0.0
30	5.7743505082727676E-5	0.0	0.0	0.39996038795551325	0.0
31	5.7743505082727676E-5	0.0	0.0	0.6233411373680453	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8560	0.0	28.441587	1
ATTGGAC	1180	0.0	22.105932	3
GTATTGG	1190	0.0	21.29832	1
TATTGGA	1545	0.0	18.200647	2
TTGGACC	1940	0.0	17.546392	4
TGGACCC	1930	0.0	16.966322	5
GGACCCT	1980	0.0	16.724747	6
GTATCAA	14715	0.0	16.59531	2
CAATGCG	155	7.223207E-9	15.5161295	19
TAGTACT	495	0.0	15.323231	4
TATACTG	475	0.0	15.189473	5
GACCCTC	2110	0.0	15.168245	7
ATTCGCG	75	0.004106624	14.8	17
CGATCGC	165	1.6483682E-8	14.575757	19
CGATCCG	90	8.279945E-4	14.388889	28
CTAGTAC	425	0.0	14.364705	3
CCCGTAT	170	2.4414476E-8	14.147059	1
GCGTTAT	275	0.0	14.127272	1
ACGTTTA	250	0.0	14.059999	26
TATACCG	290	0.0	14.034483	5
>>END_MODULE