FastQCFastQC Report
Wed 25 May 2016
SRR2088169_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088169_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199645
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT177811.4821884807588912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT169501.412917988238187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT135991.1335853523333987No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG78700.656027408108232No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52800.4401302051857008No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT46750.3896986191748392No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT45770.38152953582101373No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA44450.3705262806913712No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42890.3575224337199755No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA38370.3198446207002905No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG33650.2804996478124779No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT32320.26941303468942895No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT32090.26749580084108215No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27630.23031813578183544No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA23940.19955903621488025No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT21770.1814703516456952No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21280.17738580996878242No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19840.1653822589182633No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA19190.15996398934684844No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC17690.14746029033589103No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG17180.1432090326721655No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16510.1376240471139379No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA15940.13287264148977407No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14420.12020222649200389No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT14070.1172846967227805No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC13870.11561753685465283No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA12540.10453092373160393No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC12160.10136331998216137No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38250.031.3895421
GTATTGG3050.024.8688531
CGTCTGT2250.022.234
CTTAACC603.7264617E-521.5833323
ATTGGAC3850.020.6623363
CACCTAG450.003825904620.5555557
CGAATTA450.003825904620.55555515
CTAAACG1254.129106E-1019.24000226
GGACCCT5900.019.127126
GTATCAA64050.018.803282
CGTCTAA500.00703505618.51
AGTGCGC609.237138E-418.58
CCTAGTT1552.0008883E-1117.9032259
GTTAACG1351.1514203E-917.81481613
TTGGACC6700.017.6716424
ATCTGCG752.0673608E-417.26666636
TAATACT2050.017.1463414
GTCATAG1005.878219E-616.6500021
AAGTGTG1352.219349E-816.4444457
TATTGGA4350.016.160922
Produced by FastQC (version 0.11.5)