##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088169_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1199645
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.738506808264113	31.0	30.0	33.0	28.0	34.0
2	30.87001404582189	31.0	31.0	34.0	27.0	34.0
3	30.92554464028942	31.0	31.0	34.0	27.0	34.0
4	34.90440088526189	35.0	35.0	37.0	32.0	37.0
5	34.75838268821193	35.0	35.0	37.0	32.0	37.0
6	34.858204718896005	35.0	35.0	37.0	32.0	37.0
7	34.68388648308458	35.0	35.0	37.0	31.0	37.0
8	34.76605245718525	35.0	35.0	37.0	32.0	37.0
9	36.19578375269351	38.0	35.0	39.0	32.0	39.0
10	35.99258447290657	37.0	35.0	39.0	30.0	39.0
11	36.059662650200686	38.0	35.0	39.0	31.0	39.0
12	35.8662604353788	37.0	35.0	39.0	30.0	39.0
13	35.93014100004585	37.0	35.0	39.0	30.0	39.0
14	36.75182241413085	38.0	36.0	40.0	31.0	41.0
15	36.811174139016124	38.0	36.0	40.0	31.0	41.0
16	36.76314826469497	38.0	36.0	40.0	31.0	41.0
17	36.87557068966236	38.0	36.0	40.0	31.0	41.0
18	36.88118318335841	38.0	36.0	40.0	31.0	41.0
19	36.991307428447584	39.0	36.0	40.0	31.0	41.0
20	36.95840186055041	39.0	36.0	40.0	31.0	41.0
21	36.84820509400698	39.0	36.0	40.0	31.0	41.0
22	36.79282037602791	39.0	35.0	40.0	30.0	41.0
23	36.66281608309125	38.0	35.0	40.0	30.0	41.0
24	36.5791921776859	38.0	35.0	40.0	30.0	41.0
25	36.47528727248478	38.0	35.0	40.0	30.0	41.0
26	36.188539109486555	38.0	35.0	40.0	30.0	41.0
27	35.99837868702824	38.0	34.0	40.0	29.0	41.0
28	35.835988146493335	38.0	34.0	40.0	29.0	41.0
29	35.68590708084475	38.0	34.0	40.0	28.0	41.0
30	35.466560524155064	38.0	34.0	40.0	27.0	41.0
31	35.19699827865744	37.0	34.0	40.0	27.0	41.0
32	34.9302060192807	37.0	33.0	40.0	26.0	41.0
33	35.09880506316452	37.0	33.0	40.0	27.0	41.0
34	35.05391428297538	37.0	33.0	40.0	26.0	41.0
35	34.94847975859525	37.0	33.0	40.0	26.0	41.0
36	34.84451316847901	37.0	33.0	40.0	25.0	41.0
37	34.69870503357243	37.0	33.0	40.0	25.0	41.0
38	34.578094352912736	37.0	33.0	40.0	24.0	41.0
39	34.50815532928491	37.0	33.0	40.0	24.0	41.0
40	34.338740210645646	37.0	33.0	40.0	23.0	41.0
41	34.18131697293783	37.0	33.0	40.0	23.0	41.0
42	33.9339621304636	37.0	33.0	40.0	21.0	41.0
43	33.60294337074718	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	7.0
16	19.0
17	46.0
18	132.0
19	294.0
20	640.0
21	1297.0
22	2248.0
23	3823.0
24	6132.0
25	9676.0
26	14040.0
27	19791.0
28	26911.0
29	35509.0
30	44488.0
31	53478.0
32	64495.0
33	77143.0
34	92047.0
35	109770.0
36	136693.0
37	172606.0
38	201924.0
39	126433.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.95940465721109	23.99451504403386	14.667755877780511	21.378324420974536
2	19.021627231389285	24.15347873745983	34.925415435399636	21.899478595751244
3	21.196353921368406	26.278857495342372	32.15801341230114	20.366775170988085
4	14.131847338170875	16.961934572310973	34.78178961276044	34.12442847675771
5	10.957324875275603	41.42208736751289	34.50270705083587	13.117880706375637
6	31.712881727511053	36.66334623992931	14.901991839252446	16.721780193307186
7	24.196824894031153	36.0036510801112	21.16000983624322	18.639514189614427
8	27.797556777213263	34.24379712331565	19.89105110261786	18.067594996853238
9	25.223962088784596	13.567013574849227	23.812961334394757	37.39606300197142
10	16.602244830762434	28.19884215747159	33.40071437800349	21.79819863376249
11	31.849588836697524	21.757770006960392	24.889279745257973	21.503361411084114
12	19.994831804408804	28.0357939223687	31.599098066511345	20.370276206711154
13	31.43171521575133	20.733466983982762	29.13095123974176	18.703866560524155
14	20.791484147393604	22.558256817641887	28.925640501981835	27.724618532982674
15	23.314063743857556	29.12244872441431	27.06609038507225	20.497397146655885
16	21.652572219281538	28.34505207790638	27.613585685765372	22.38879001704671
17	19.290540118118276	27.44595276102514	28.449583001637986	24.8139241192186
18	20.283500535575108	26.159238774804212	32.91740473223329	20.639855957387393
19	19.654731191310763	24.74623742857262	34.53696718612589	21.06206419399072
20	23.939915558352677	23.69150873800166	33.418386272605645	18.950189431040016
21	23.54238128779764	24.345035406307698	32.042562591433295	20.07002071446136
22	21.746933467817563	24.20282666955641	32.75994148268863	21.290298379937397
23	20.827911590512194	26.19083145430523	33.7285613660708	19.25269558911178
24	19.44000100029592	25.29631682706134	32.32606312700841	22.93761904563433
25	20.531073775992066	26.287610084650044	33.426972145926506	19.75434399343139
26	21.945992356072004	25.21379241358902	31.984378712035642	20.85583651830333
27	20.92085575316031	26.713569430956657	31.453971799990832	20.9116030158922
28	20.533991305761287	26.192581972166767	31.830416498214053	21.443010223857893
29	19.71199813278095	25.920001333727893	32.670164923789955	21.6978356097012
30	18.978947938765216	26.166324204243757	34.71677037790346	20.13795747908756
31	20.3800290919397	25.683598064427393	33.3066865614411	20.629686282191813
32	21.348648975321865	26.114058742377956	31.301676746037373	21.235615536262813
33	22.35419644978306	26.034285142688045	30.710668572786116	20.900849834742775
34	20.73946875950802	28.610047138945273	30.962826502840425	19.687657598706284
35	19.290956908085306	29.453546674224455	31.099366896040078	20.156129521650154
36	20.472556464620784	25.803633574932583	33.1970708001117	20.526739160334934
37	20.354771619937566	26.201667993448062	32.37849530486102	21.06506508175335
38	20.89251403540214	25.723443185275645	32.70675908289536	20.67728369642686
39	21.64190239612552	25.4055157984237	32.53737564029359	20.415206165157194
40	19.741173430473182	26.404978139366232	31.655114638080434	22.19873379208016
41	20.630186430152254	25.921835209582834	31.63794289143872	21.810035468826193
42	18.37860367025245	27.645761871220238	31.75022610855712	22.2254083499702
43	19.05363670085734	27.293407633091455	31.39061972500198	22.262335941049226
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	61.0
1	101.0
2	141.0
3	370.5
4	600.0
5	600.0
6	1194.5
7	1789.0
8	2034.0
9	2279.0
10	3535.5
11	4792.0
12	4792.0
13	9260.5
14	13729.0
15	23760.0
16	33791.0
17	32513.5
18	31236.0
19	31236.0
20	34091.0
21	36946.0
22	29381.0
23	21816.0
24	23501.0
25	25186.0
26	25186.0
27	27232.0
28	29278.0
29	31080.0
30	32882.0
31	35298.0
32	37714.0
33	37714.0
34	41546.5
35	45379.0
36	49768.5
37	54158.0
38	55564.5
39	56971.0
40	56971.0
41	59677.0
42	62383.0
43	71460.0
44	80537.0
45	100282.5
46	120028.0
47	120028.0
48	121568.0
49	123108.0
50	110819.5
51	98531.0
52	90550.5
53	82570.0
54	82570.0
55	71754.0
56	60938.0
57	50907.5
58	40877.0
59	35767.0
60	30657.0
61	30657.0
62	26901.5
63	23146.0
64	19834.0
65	16522.0
66	13973.0
67	11424.0
68	11424.0
69	9647.5
70	7871.0
71	6542.5
72	5214.0
73	4104.5
74	2995.0
75	2995.0
76	2404.5
77	1814.0
78	1422.0
79	1030.0
80	798.0
81	566.0
82	566.0
83	439.0
84	312.0
85	234.5
86	157.0
87	138.0
88	119.0
89	119.0
90	99.0
91	79.0
92	45.5
93	12.0
94	9.5
95	7.0
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1199645.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.984188713328116
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.21899792013389	39.294625325139144
2	11.285956264508249	11.056668229420794
3	3.4893822628541584	5.12773677769764
4	1.5021492863781793	2.943262564781596
5	0.7591638809098131	1.8593513403414414
6	0.4881183662144978	1.4346049299055414
7	0.32632205497055233	1.1189234785398998
8	0.2641201452927066	1.0350168832007667
9	0.197294024288266	0.8697858945973527
>10	1.2785740695295884	12.09740074506778
>50	0.11485622186833495	3.9026655809513247
>100	0.06118352788394946	5.652960599823096
>500	0.0073694436076553235	2.6230265886264212
>1k	0.00565561951285176	5.8227076703645375
>5k	3.4276481896071274E-4	1.1039462198891803
>10k+	5.141472284410691E-4	4.057317171653542
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17781	1.4821884807588912	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16950	1.412917988238187	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13599	1.1335853523333987	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7870	0.656027408108232	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5280	0.4401302051857008	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4675	0.3896986191748392	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4577	0.38152953582101373	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4445	0.3705262806913712	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4289	0.3575224337199755	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3837	0.3198446207002905	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3365	0.2804996478124779	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3232	0.26941303468942895	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3209	0.26749580084108215	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2763	0.23031813578183544	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2394	0.19955903621488025	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2177	0.1814703516456952	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2128	0.17738580996878242	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1984	0.1653822589182633	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1919	0.15996398934684844	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1769	0.14746029033589103	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1718	0.1432090326721655	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1651	0.1376240471139379	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1594	0.13287264148977407	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1442	0.12020222649200389	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1407	0.1172846967227805	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1387	0.11561753685465283	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1254	0.10453092373160393	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1216	0.10136331998216137	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.6671598681276543E-4	0.0
11	0.0	0.0	0.0	2.5007398021914816E-4	0.0
12	0.0	0.0	0.0	7.502219406574445E-4	0.0
13	0.0	0.0	0.0	8.335799340638272E-4	0.0
14	0.0	0.0	0.0	9.1693792747021E-4	0.0
15	0.0	0.0	0.0	0.0013337278945021235	0.0
16	0.0	0.0	0.0	0.002334023815378716	0.0
17	0.0	0.0	0.0	0.004001183683506371	0.0
18	8.335799340638272E-5	0.0	0.0	0.004501331643944667	0.0
19	8.335799340638272E-5	0.0	0.0	0.005584985558227643	0.0
20	8.335799340638272E-5	0.0	0.0	0.007668935393387211	0.0
21	8.335799340638272E-5	0.0	0.0	0.009752885228546778	0.0
22	8.335799340638272E-5	0.0	0.0	0.017588536608746753	0.0
23	8.335799340638272E-5	0.0	0.0	0.028091643777950977	0.0
24	8.335799340638272E-5	0.0	0.0	0.04526339041966582	0.0
25	8.335799340638272E-5	0.0	0.0	0.05168195591195729	0.0
26	8.335799340638272E-5	0.0	0.0	0.059350891305344496	0.0
27	8.335799340638272E-5	0.0	0.0	0.11161635317114646	0.0
28	8.335799340638272E-5	0.0	0.0	0.17455163819296543	0.0
29	8.335799340638272E-5	0.0	0.0	0.24590608054882904	0.0
30	8.335799340638272E-5	0.0	0.0	0.3989513564429477	0.0
31	8.335799340638272E-5	0.0	0.0	0.6268521104159981	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3825	0.0	31.389542	1
GTATTGG	305	0.0	24.868853	1
CGTCTGT	225	0.0	22.2	34
CTTAACC	60	3.7264617E-5	21.583332	3
ATTGGAC	385	0.0	20.662336	3
CACCTAG	45	0.0038259046	20.555555	7
CGAATTA	45	0.0038259046	20.555555	15
CTAAACG	125	4.129106E-10	19.240002	26
GGACCCT	590	0.0	19.12712	6
GTATCAA	6405	0.0	18.80328	2
CGTCTAA	50	0.007035056	18.5	1
AGTGCGC	60	9.237138E-4	18.5	8
CCTAGTT	155	2.0008883E-11	17.903225	9
GTTAACG	135	1.1514203E-9	17.814816	13
TTGGACC	670	0.0	17.671642	4
ATCTGCG	75	2.0673608E-4	17.266666	36
TAATACT	205	0.0	17.146341	4
GTCATAG	100	5.878219E-6	16.650002	1
AAGTGTG	135	2.219349E-8	16.444445	7
TATTGGA	435	0.0	16.16092	2
>>END_MODULE