FastQCFastQC Report
Wed 25 May 2016
SRR2088168_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088168_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3409216
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT429371.2594391203138786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT424241.2443916724548987No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT381201.11814563817605No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG240610.7057634365203026No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140460.41200088231429166No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA133650.39202561527342356No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT121660.3568562390884004No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT119170.3495525070866733No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA111770.3278466368807374No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA99940.293146576808275No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT94400.27689650641085806No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG90640.2658675777656798No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT90590.2657209164805046No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA74590.21878930522442694No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA72800.21353883121515327No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA72310.21210155062043592No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT65530.192214280350673No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA51150.15003449473427322No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC50960.1494771818506073No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50840.14912519476618671No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG46220.1355736920159943No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA46000.13492838236122323No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG43700.12818196324316206No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC41420.12149420863917101No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT40550.11894230227712178No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.10926265745555576No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA35680.10465749310105314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91400.030.988511
GTATTGG10400.022.769231
ATTGGAC11200.021.6383913
CGACGTA500.00703765118.520
TTGGACC19200.018.2109384
TATACCG2150.017.2093035
TATTGGA14100.017.1879432
GGACCCT20300.017.1330056
CGTCTGT9400.017.1223434
GTATCAA167450.016.9587942
CTAGTAC3900.016.6025623
GACCCTC20200.016.5767337
TGGACCC21400.016.5116835
TAAACGT2003.6379788E-1215.7254
ACGTGAT2250.015.6222227
TATACTG4900.015.4795925
ACGTTTA2055.456968E-1215.34146426
GTACGTT1101.4534551E-515.1363644
TCACGTT2201.8189894E-1215.13636424
TGTGCGC2700.015.07407310
Produced by FastQC (version 0.11.5)