##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088168_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3409216
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.133909086429256	31.0	31.0	33.0	30.0	34.0
2	31.276432470104563	31.0	31.0	34.0	28.0	34.0
3	31.323453251422027	31.0	31.0	34.0	28.0	34.0
4	35.28787821012221	37.0	35.0	37.0	33.0	37.0
5	35.163199691659315	37.0	35.0	37.0	32.0	37.0
6	35.2155466242092	37.0	35.0	37.0	32.0	37.0
7	35.05493169103982	37.0	35.0	37.0	32.0	37.0
8	35.11465216636318	37.0	35.0	37.0	32.0	37.0
9	36.61746718307083	39.0	35.0	39.0	32.0	39.0
10	36.46766939965083	38.0	35.0	39.0	32.0	39.0
11	36.499325651410764	38.0	35.0	39.0	32.0	39.0
12	36.334749983573936	38.0	35.0	39.0	32.0	39.0
13	36.38272406324504	38.0	35.0	39.0	32.0	39.0
14	37.31774196765473	39.0	36.0	41.0	32.0	41.0
15	37.378785621092945	39.0	36.0	41.0	32.0	41.0
16	37.3619896773921	39.0	36.0	41.0	32.0	41.0
17	37.45953849800071	39.0	36.0	41.0	32.0	41.0
18	37.46959887551859	39.0	36.0	41.0	32.0	41.0
19	37.563406366742385	39.0	36.0	41.0	32.0	41.0
20	37.52714964378907	39.0	36.0	41.0	32.0	41.0
21	37.40988807984006	39.0	36.0	41.0	32.0	41.0
22	37.370242014586346	39.0	36.0	41.0	32.0	41.0
23	37.2581112490379	39.0	36.0	41.0	31.0	41.0
24	37.18461165264976	39.0	36.0	41.0	31.0	41.0
25	37.09706161181926	39.0	36.0	40.0	31.0	41.0
26	36.83769112898684	39.0	35.0	40.0	31.0	41.0
27	36.686852637087235	39.0	35.0	40.0	30.0	41.0
28	36.54895231044322	38.0	35.0	40.0	30.0	41.0
29	36.419072009517734	38.0	35.0	40.0	30.0	41.0
30	36.24705328145826	38.0	35.0	40.0	30.0	41.0
31	35.995846845726405	38.0	35.0	40.0	30.0	41.0
32	35.715116613321065	38.0	34.0	40.0	29.0	41.0
33	35.85923831168222	38.0	34.0	40.0	29.0	41.0
34	35.8367190579887	38.0	35.0	40.0	29.0	41.0
35	35.74959521485292	38.0	35.0	40.0	29.0	41.0
36	35.661250269856765	38.0	34.0	40.0	28.0	41.0
37	35.523745342037586	38.0	34.0	40.0	27.0	41.0
38	35.40217457620755	38.0	34.0	40.0	27.0	41.0
39	35.3488221338865	38.0	34.0	40.0	26.0	41.0
40	35.1884794040624	38.0	34.0	40.0	25.0	41.0
41	35.04899396224821	38.0	34.0	40.0	24.0	41.0
42	34.83505826559537	38.0	34.0	40.0	24.0	41.0
43	34.52696719715031	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	2.0
14	2.0
15	14.0
16	34.0
17	95.0
18	218.0
19	512.0
20	1121.0
21	2143.0
22	4040.0
23	7119.0
24	11671.0
25	18658.0
26	28787.0
27	42100.0
28	59233.0
29	81229.0
30	104205.0
31	128377.0
32	157320.0
33	193270.0
34	235798.0
35	286290.0
36	359633.0
37	474639.0
38	595925.0
39	616779.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.66272128254707	22.807912434999718	14.110927556364866	22.418438726088343
2	17.222288056843567	23.109360040548914	37.02604939082769	22.642302511779835
3	19.50328169291708	26.26090573316563	32.58068717265201	21.655125401265277
4	12.652205081754866	15.740950412059545	35.875843595712325	35.73100091047326
5	11.096598162158102	40.60939523925736	34.891101062531675	13.402905536052865
6	31.282588137565938	36.5019112898684	15.587542707766245	16.627957864799413
7	24.600758649495955	35.470823790572375	21.4753773301545	18.45304022977717
8	27.605085744053763	33.75867061517956	20.24779304098068	18.38845059978599
9	25.213010850588525	13.744274343426758	23.994959544951097	37.04775526103362
10	16.211498479415795	27.72625729786555	33.94715969888678	22.11508452383187
11	31.367270363626126	21.911225337438285	25.556344919183765	21.165159379751824
12	20.360047588653813	27.41002623477069	31.96186454598359	20.2680616305919
13	31.086795321856993	20.956049719348965	28.932634365203025	19.024520593591017
14	20.785247986633877	22.692607332594942	28.831027426833618	27.691117253937563
15	22.834194137303122	29.152244973624438	27.172992265670466	20.84056862340198
16	21.601476703148172	28.722351414518766	27.32041618952862	22.355755692804447
17	19.26545575287691	27.303520809476435	28.222881741725956	25.208141695920705
18	20.343474863429012	26.63187078788789	32.68091549494077	20.343738853742327
19	19.59174778013479	24.98357393606037	34.13852334378344	21.2861549400214
20	24.18095538681034	23.812160919108674	32.677278295068426	19.329605399012557
21	23.39681615949239	24.96688388180743	31.47368779214928	20.162612166550904
22	21.751393868854304	24.543296757964296	32.35013563235653	21.35517374082487
23	20.81064972122623	26.374568229176443	33.376823293097296	19.43795875650003
24	19.270266243030658	25.55690223206743	31.853481856239092	23.319349668662824
25	20.730103343407986	26.451154752294954	32.93340756349847	19.88533434079859
26	22.04157788770204	25.49028867634084	31.547575747620566	20.920557688336554
27	20.53038000525634	27.01345411965684	31.204124349997187	21.25204152508964
28	20.416717509245526	26.35693954269838	31.291798466274944	21.93454448178115
29	19.41384764121722	26.315317069965644	32.360724577146186	21.910110711670953
30	18.89100015956748	26.605530421070416	34.24271152077193	20.260757898590175
31	20.46784363325762	26.05874781768008	32.889497174717	20.583911374345305
32	21.54864930822805	26.152523043421127	30.933240956278514	21.36558669207231
33	22.413628235934596	26.106618061161274	30.432539328690233	21.047214374213898
34	20.606907863860783	28.83445930090672	30.511325771086373	20.04730706414613
35	19.271410201055023	29.821548414650174	30.77305750061011	20.133983883684696
36	20.297129897313635	26.029826212243517	32.928831731401004	20.744212159041844
37	20.319950393286902	26.33828422722409	32.04584279787493	21.29592258161407
38	20.62172065366348	25.914579774352813	32.67974220465937	20.783957367324334
39	21.535684450618557	25.426549681803674	32.49914349809458	20.53862236948319
40	19.74257424580901	26.308746644389796	31.612986680808724	22.335692428992473
41	20.7024136927669	25.815583406859528	31.511321077925246	21.97068182244833
42	18.196382980720493	27.630340817360942	31.95288887533087	22.220387326587694
43	18.91942311663444	27.375766158553756	31.48820725938163	22.216603465430175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	214.0
1	334.0
2	454.0
3	1408.5
4	2363.0
5	2363.0
6	4193.0
7	6023.0
8	6372.0
9	6721.0
10	10469.0
11	14217.0
12	14217.0
13	25922.0
14	37627.0
15	62413.0
16	87199.0
17	86803.0
18	86407.0
19	86407.0
20	92459.0
21	98511.0
22	81537.5
23	64564.0
24	70180.5
25	75797.0
26	75797.0
27	82710.0
28	89623.0
29	93402.0
30	97181.0
31	103726.0
32	110271.0
33	110271.0
34	120064.5
35	129858.0
36	140598.5
37	151339.0
38	156475.5
39	161612.0
40	161612.0
41	168373.0
42	175134.0
43	200028.0
44	224922.0
45	283762.5
46	342603.0
47	342603.0
48	344447.0
49	346291.0
50	313809.5
51	281328.0
52	260908.0
53	240488.0
54	240488.0
55	208330.0
56	176172.0
57	144093.0
58	112014.0
59	98185.5
60	84357.0
61	84357.0
62	74308.0
63	64259.0
64	55959.0
65	47659.0
66	40610.0
67	33561.0
68	33561.0
69	28475.0
70	23389.0
71	19463.5
72	15538.0
73	12422.5
74	9307.0
75	9307.0
76	7328.0
77	5349.0
78	4127.5
79	2906.0
80	2406.0
81	1906.0
82	1906.0
83	1378.5
84	851.0
85	696.0
86	541.0
87	471.5
88	402.0
89	402.0
90	297.5
91	193.0
92	117.5
93	42.0
94	31.0
95	20.0
96	20.0
97	11.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3409216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.12102892979816
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.85205340769546	26.30101124282668
2	14.261697764539228	10.588177906109966
3	5.814889419450031	6.475640350889454
4	2.8474921745886688	4.228073575611193
5	1.6032636968315968	2.9757399036090417
6	1.0322871013546686	2.2991735611946473
7	0.6528330878832537	1.6963685159150605
8	0.4625973745609321	1.3737672419140028
9	0.32143021099585334	1.0738638145159378
>10	1.826936778069658	12.76871544183433
>50	0.1798953122465658	4.655445812522239
>100	0.12529983137619616	8.718672593767664
>500	0.010496900962426203	2.6379104855491557
>1k	0.007236499905844234	5.599943720449962
>5k	8.74741746860292E-4	2.4021392287543524
>10k+	7.156977928856935E-4	6.2053566045362665
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	42937	1.2594391203138786	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42424	1.2443916724548987	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38120	1.11814563817605	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	24061	0.7057634365203026	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14046	0.41200088231429166	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13365	0.39202561527342356	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	12166	0.3568562390884004	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11917	0.3495525070866733	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11177	0.3278466368807374	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9994	0.293146576808275	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	9440	0.27689650641085806	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9064	0.2658675777656798	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	9059	0.2657209164805046	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7459	0.21878930522442694	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7280	0.21353883121515327	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7231	0.21210155062043592	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6553	0.192214280350673	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5115	0.15003449473427322	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5096	0.1494771818506073	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5084	0.14912519476618671	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4622	0.1355736920159943	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4600	0.13492838236122323	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4370	0.12818196324316206	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4142	0.12149420863917101	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4055	0.11894230227712178	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3725	0.10926265745555576	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3568	0.10465749310105314	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	2.933225703504853E-5	0.0
4	0.0	0.0	0.0	2.933225703504853E-5	0.0
5	2.933225703504853E-5	0.0	0.0	2.933225703504853E-5	0.0
6	2.933225703504853E-5	0.0	0.0	2.933225703504853E-5	0.0
7	2.933225703504853E-5	0.0	0.0	2.933225703504853E-5	0.0
8	8.799677110514559E-5	0.0	0.0	2.933225703504853E-5	0.0
9	8.799677110514559E-5	0.0	0.0	1.1732902814019411E-4	0.0
10	8.799677110514559E-5	0.0	0.0	2.053257992453397E-4	0.0
11	8.799677110514559E-5	0.0	0.0	4.399838555257279E-4	0.0
12	8.799677110514559E-5	0.0	0.0	8.213031969813587E-4	0.0
13	8.799677110514559E-5	0.0	0.0	9.679644821566014E-4	0.0
14	8.799677110514559E-5	0.0	0.0	0.0012026225384369896	0.0
15	8.799677110514559E-5	0.0	0.0	0.001554609622857572	0.0
16	8.799677110514559E-5	0.0	0.0	0.002346580562803882	0.0
17	8.799677110514559E-5	0.0	0.0	0.0032558805308903864	0.0
18	8.799677110514559E-5	0.0	0.0	0.00378386115752126	0.0
19	8.799677110514559E-5	0.0	0.0	0.004399838555257279	0.0
20	8.799677110514559E-5	0.0	0.0	0.005661125607764366	0.0
21	8.799677110514559E-5	0.0	0.0	0.008330360997953782	0.0
22	8.799677110514559E-5	0.0	0.0	0.014314141433103682	0.0
23	8.799677110514559E-5	0.0	0.0	0.024521766881300568	0.0
24	8.799677110514559E-5	0.0	0.0	0.03939322119807017	0.0
25	8.799677110514559E-5	0.0	0.0	0.045552995175430364	0.0
26	8.799677110514559E-5	0.0	0.0	0.05353136908896356	0.0
27	8.799677110514559E-5	0.0	0.0	0.11823832810828061	0.0
28	8.799677110514559E-5	0.0	0.0	0.1881077643657662	0.0
29	8.799677110514559E-5	0.0	0.0	0.27525390001689537	0.0
30	8.799677110514559E-5	0.0	0.0	0.44787423266815596	0.0
31	8.799677110514559E-5	0.0	0.0	0.7143871200886068	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9140	0.0	30.98851	1
GTATTGG	1040	0.0	22.76923	1
ATTGGAC	1120	0.0	21.638391	3
CGACGTA	50	0.007037651	18.5	20
TTGGACC	1920	0.0	18.210938	4
TATACCG	215	0.0	17.209303	5
TATTGGA	1410	0.0	17.187943	2
GGACCCT	2030	0.0	17.133005	6
CGTCTGT	940	0.0	17.12234	34
GTATCAA	16745	0.0	16.958794	2
CTAGTAC	390	0.0	16.602562	3
GACCCTC	2020	0.0	16.576733	7
TGGACCC	2140	0.0	16.511683	5
TAAACGT	200	3.6379788E-12	15.725	4
ACGTGAT	225	0.0	15.622222	7
TATACTG	490	0.0	15.479592	5
ACGTTTA	205	5.456968E-12	15.341464	26
GTACGTT	110	1.4534551E-5	15.136364	4
TCACGTT	220	1.8189894E-12	15.136364	24
TGTGCGC	270	0.0	15.074073	10
>>END_MODULE