FastQCFastQC Report
Wed 25 May 2016
SRR2088167_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088167_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1649160
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT288821.751315821387858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT288661.7503456305028013No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT238791.4479492590167116No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG116660.7073904290669188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96880.5874505809017925No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT78850.4781221955419729No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT68120.4130587693128623No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA67560.4096631012151641No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58640.35557495937325667No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT56620.34332629944941667No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA56120.34029445293361466No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT51230.31064299400907125No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG51170.310279172427175No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA44100.267408862693735No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43060.26110262194086686No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT39750.24103179800625774No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35810.21714084746173812No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA34520.20931868345096896No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34100.2067719323776953No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31160.18894467486477964No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30370.18415435736981253No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC29040.17608964563777924No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG27190.16487181352931188No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC23860.14467971573407068No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG23200.14067767833321204No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT23100.14007130903005166No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23080.1399500351694196No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC22560.1367969147929855No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA22530.13661500400203738No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22230.1347958960925562No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC21060.12770137524557956No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA21050.12764073831526354No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC20310.12315360547187658No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC20080.12175895607460768No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA19510.11830265104659343No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT19250.11672609085837639No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG18870.11442188750636687No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC18760.11375488127289045No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT18540.11242086880593757No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA17590.10666036042591379No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC17430.10569016954085717No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA17060.10344660311916369No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.030.8606911
ATTGGAC3600.025.6944453
TCCGACG451.3235532E-424.66666623
GTATTGG3650.024.3287681
GAACCGT551.9033101E-523.5454546
AACCGTG502.702794E-422.1999997
CGTCTGT5700.021.42105334
GCGCGCT806.963892E-720.812523
GGATCGT450.00382650320.55555512
AACGGTA450.00382650320.55555518
GGACCCT8250.019.7333346
TATTGGA5000.019.242
TTGGACC9050.019.011054
CTAAACG801.6173653E-518.526
CGGGATA902.1533706E-618.524
CTGTGCG609.2391396E-418.59
TCGCACG500.0070361518.49999822
GACCCTC8450.018.3905337
ACGATCT2150.018.06976932
TATACCG953.608311E-617.5263165
Produced by FastQC (version 0.11.5)