##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088167_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1649160
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.091482936767807	31.0	31.0	33.0	30.0	34.0
2	31.22198391908608	31.0	31.0	34.0	28.0	34.0
3	31.245046569162483	31.0	31.0	34.0	28.0	34.0
4	35.213364985810955	37.0	35.0	37.0	33.0	37.0
5	35.10396262339615	35.0	35.0	37.0	32.0	37.0
6	35.172783720196946	37.0	35.0	37.0	32.0	37.0
7	34.99957069053336	37.0	35.0	37.0	32.0	37.0
8	35.07557059351427	37.0	35.0	37.0	32.0	37.0
9	36.52528984452691	39.0	35.0	39.0	32.0	39.0
10	36.40028014261806	38.0	35.0	39.0	32.0	39.0
11	36.44306434791045	38.0	35.0	39.0	32.0	39.0
12	36.28293919328628	38.0	35.0	39.0	32.0	39.0
13	36.34422251327949	38.0	35.0	39.0	32.0	39.0
14	37.2354750297121	39.0	36.0	41.0	32.0	41.0
15	37.29665708603168	39.0	36.0	41.0	32.0	41.0
16	37.26821290838973	39.0	36.0	41.0	32.0	41.0
17	37.416895267893956	39.0	36.0	41.0	32.0	41.0
18	37.44423585340416	39.0	36.0	41.0	32.0	41.0
19	37.55902883892406	39.0	36.0	41.0	32.0	41.0
20	37.526805767784815	39.0	36.0	41.0	32.0	41.0
21	37.41539086565282	39.0	36.0	41.0	32.0	41.0
22	37.374636784787405	39.0	36.0	41.0	32.0	41.0
23	37.2708172645468	39.0	36.0	41.0	31.0	41.0
24	37.1938411069878	39.0	36.0	41.0	31.0	41.0
25	37.102412137087974	39.0	36.0	40.0	31.0	41.0
26	36.80766208251473	39.0	35.0	40.0	31.0	41.0
27	36.61825232239443	38.0	35.0	40.0	30.0	41.0
28	36.44281088554173	38.0	35.0	40.0	30.0	41.0
29	36.27984489073225	38.0	35.0	40.0	30.0	41.0
30	36.08354010526571	38.0	35.0	40.0	30.0	41.0
31	35.75634808023479	38.0	34.0	40.0	29.0	41.0
32	35.45335382861578	38.0	34.0	40.0	28.0	41.0
33	35.51067270610493	38.0	34.0	40.0	28.0	41.0
34	35.43817337311116	38.0	34.0	40.0	27.0	41.0
35	35.3190333260569	38.0	34.0	40.0	27.0	41.0
36	35.18312838050886	38.0	34.0	40.0	26.0	41.0
37	35.000858012563974	38.0	34.0	40.0	25.0	41.0
38	34.822533289674745	38.0	33.0	40.0	24.0	41.0
39	34.67151216376822	38.0	33.0	40.0	24.0	41.0
40	34.42725266196124	38.0	33.0	40.0	22.0	41.0
41	34.2258434597007	38.0	33.0	40.0	21.0	41.0
42	33.94636966698198	38.0	33.0	40.0	18.0	41.0
43	33.58628453273182	37.0	33.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	7.0
16	17.0
17	43.0
18	143.0
19	318.0
20	701.0
21	1330.0
22	2463.0
23	4147.0
24	6794.0
25	10675.0
26	16042.0
27	23300.0
28	32860.0
29	43379.0
30	54778.0
31	66019.0
32	79730.0
33	97264.0
34	117560.0
35	140181.0
36	174546.0
37	229154.0
38	266894.0
39	280814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.06003056101288	23.68902956656722	15.329561716267676	20.921378156152223
2	17.172075480850857	23.76779693904776	36.71323582914939	22.346891750952
3	20.23933396395741	26.081095830604674	33.18228673991608	20.497283465521843
4	12.981093405127458	15.815991171262947	34.493014625627595	36.709900797982
5	10.280930898154212	41.95899730772029	34.146777753523004	13.613294040602488
6	31.03719469305586	35.49734410245215	15.372250115210168	18.093211089281816
7	23.117162676756653	37.27685609643697	20.92719930146256	18.678781925343813
8	28.140871716510222	33.47752795362487	20.21344199471246	18.16815833515244
9	24.812207426811224	13.906655509471488	25.665672221009483	35.6154648427078
10	16.538844017560457	28.810060879478037	33.19593004923719	21.455165053724322
11	29.983931213466246	22.12550631836814	25.94029687841083	21.950265589754782
12	19.928872880739284	28.814911833903324	31.906182541415024	19.35003274394237
13	31.124390598850322	21.600329864900917	30.32465012491208	16.95062941133668
14	20.264134468456668	23.722379878241043	30.174452448519247	25.83903320478304
15	22.38763976812438	29.633085934657643	28.55417303354435	19.42510126367363
16	20.59224089839676	28.79653884401756	28.933699580392442	21.677520677193236
17	17.877949986659875	27.879344636057148	29.266656964757814	24.976048412525166
18	18.821096800795555	26.91679400422033	35.01182420141163	19.250284993572485
19	18.082538989546194	25.291966819471728	36.823049309951735	19.802444881030343
20	23.477527953624875	23.686179630842368	35.247156127968175	17.58913628756458
21	22.61587717383395	24.974956947779475	33.5763055131097	18.83286036527687
22	20.258373960076646	24.73277304809721	34.602403647917725	20.406449343908413
23	19.215843217152976	27.23774527638313	35.768209270173905	17.77820223628999
24	17.492238472919546	26.190545489825123	33.90883843896287	22.408377598292464
25	18.8813699095297	27.3499235974678	35.59533338184288	18.17337311115962
26	20.693565208954862	25.843277789905162	33.775982924640424	19.68717407649955
27	19.1826748162701	27.621213223701762	33.0833272696403	20.112784690387834
28	18.751424967862427	27.147214339421282	33.24601615367824	20.855344539038057
29	17.504790317494965	27.027395765116786	34.53067015935385	20.937143758034395
30	16.902604962526375	27.006779208809334	37.259999029809116	18.830616798855175
31	18.617963084236823	26.47080938174586	35.451320672342284	19.459906861675037
32	20.135584176186665	26.862948410099683	33.071745615949936	19.929721797763712
33	21.24329961920008	26.73227582526862	32.21561279681777	19.808811758713528
34	18.959773460428337	30.06603361711417	32.40643721652235	18.567755705935145
35	17.19960464721434	30.832363142448276	32.589924567658684	19.378107642678696
36	18.423621722573916	26.432608115646754	35.329561716267676	19.814208445511657
37	18.479043876882777	26.694680928472675	34.38144267384608	20.444832520798467
38	19.035933444905282	26.359358703824974	34.854895825753715	19.749812025516018
39	20.398142084455117	25.630320890635232	34.48682965873536	19.484707366174295
40	18.191564190254432	26.569465667370057	33.577033156273494	21.661936986102013
41	19.075408086541028	26.12032792451915	33.3513425016372	21.452921487302625
42	16.23650828300468	27.94865264740838	33.819277692886075	21.995561376700863
43	16.92091731548182	27.731693710737588	33.54434985083315	21.80303912294744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	237.5
2	302.0
3	932.0
4	1562.0
5	1562.0
6	2853.0
7	4144.0
8	4672.5
9	5201.0
10	7764.5
11	10328.0
12	10328.0
13	18765.5
14	27203.0
15	44519.5
16	61836.0
17	59483.5
18	57131.0
19	57131.0
20	60414.5
21	63698.0
22	50330.0
23	36962.0
24	38854.0
25	40746.0
26	40746.0
27	43022.0
28	45298.0
29	45663.0
30	46028.0
31	48875.0
32	51722.0
33	51722.0
34	55358.5
35	58995.0
36	63278.0
37	67561.0
38	69475.5
39	71390.0
40	71390.0
41	74796.0
42	78202.0
43	92220.5
44	106239.0
45	142953.0
46	179667.0
47	179667.0
48	179656.5
49	179646.0
50	159010.0
51	138374.0
52	124231.0
53	110088.0
54	110088.0
55	93137.5
56	76187.0
57	58914.0
58	41641.0
59	35409.5
60	29178.0
61	29178.0
62	24933.5
63	20689.0
64	17401.5
65	14114.0
66	11776.0
67	9438.0
68	9438.0
69	7741.5
70	6045.0
71	5022.5
72	4000.0
73	3161.5
74	2323.0
75	2323.0
76	1816.5
77	1310.0
78	1023.5
79	737.0
80	617.0
81	497.0
82	497.0
83	368.0
84	239.0
85	187.0
86	135.0
87	100.5
88	66.0
89	66.0
90	58.0
91	50.0
92	30.5
93	11.0
94	7.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1649160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.333805877094896
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.29055713510809	25.902572638169595
2	13.790872819203972	10.02149791777323
3	5.64094644755859	6.148711595660455
4	2.922300951622166	4.247132619627577
5	1.6952442406292383	3.0797337576642945
6	1.0086145028581022	2.198808213100132
7	0.6446356830713249	1.639544743911339
8	0.47193110156601903	1.371764242533063
9	0.35060527107605427	1.146494147288723
>10	1.8897013809673882	12.834730534825958
>50	0.1700463122949914	4.310839850322967
>100	0.09909605446993985	7.182576294001652
>500	0.012302723635177374	3.1015395510663657
>1k	0.01095447994913054	7.518069168649685
>5k	0.00151677414680269	3.583324968684777
>10k+	6.741218430234177E-4	5.712659756720193
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28882	1.751315821387858	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28866	1.7503456305028013	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23879	1.4479492590167116	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11666	0.7073904290669188	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9688	0.5874505809017925	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7885	0.4781221955419729	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6812	0.4130587693128623	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6756	0.4096631012151641	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5864	0.35557495937325667	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5662	0.34332629944941667	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5612	0.34029445293361466	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5123	0.31064299400907125	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5117	0.310279172427175	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4410	0.267408862693735	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4306	0.26110262194086686	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3975	0.24103179800625774	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3581	0.21714084746173812	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3452	0.20931868345096896	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3410	0.2067719323776953	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3116	0.18894467486477964	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3037	0.18415435736981253	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2904	0.17608964563777924	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2719	0.16487181352931188	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2386	0.14467971573407068	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2320	0.14067767833321204	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2310	0.14007130903005166	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2308	0.1399500351694196	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2256	0.1367969147929855	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2253	0.13661500400203738	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2223	0.1347958960925562	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2106	0.12770137524557956	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2105	0.12764073831526354	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	2031	0.12315360547187658	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	2008	0.12175895607460768	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1951	0.11830265104659343	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	1925	0.11672609085837639	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1887	0.11442188750636687	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	1876	0.11375488127289045	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	1854	0.11242086880593757	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1759	0.10666036042591379	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	1743	0.10569016954085717	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1706	0.10344660311916369	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.063693031603968E-5	0.0
5	0.0	0.0	0.0	6.063693031603968E-5	0.0
6	1.8191079094811904E-4	0.0	0.0	6.063693031603968E-5	0.0
7	1.8191079094811904E-4	0.0	0.0	1.2127386063207936E-4	0.0
8	1.8191079094811904E-4	0.0	0.0	1.2127386063207936E-4	0.0
9	1.8191079094811904E-4	0.0	0.0	1.2127386063207936E-4	0.0
10	1.8191079094811904E-4	0.0	0.0	3.638215818962381E-4	0.0
11	1.8191079094811904E-4	0.0	0.0	7.276431637924761E-4	0.0
12	2.4254772126415872E-4	0.0	0.0	9.095539547405952E-4	0.0
13	3.031846515801984E-4	0.0	0.0	9.095539547405952E-4	0.0
14	3.638215818962381E-4	0.0	0.0	0.0010914647456887142	0.0
15	3.638215818962381E-4	0.0	0.0	0.0017584709791651507	0.0
16	4.2445851221227775E-4	0.0	0.0	0.0031531203764340633	0.0
17	4.2445851221227775E-4	0.0	0.0	0.004608406704019016	0.0
18	4.2445851221227775E-4	0.0	0.0	0.005275412937495452	0.0
19	4.2445851221227775E-4	0.0	0.0	0.006427514613500206	0.0
20	4.2445851221227775E-4	0.0	0.0	0.008125348662349317	0.0
21	4.2445851221227775E-4	0.0	0.0	0.011642290620679618	0.0
22	4.2445851221227775E-4	0.0	0.0	0.019403817701132697	0.0
23	4.2445851221227775E-4	0.0	0.0	0.03189502534623687	0.0
24	4.2445851221227775E-4	0.0	0.0	0.050692473744209175	0.0
25	4.2445851221227775E-4	0.0	0.0	0.06087947803730384	0.0
26	4.2445851221227775E-4	0.0	0.0	0.07470469814936088	0.0
27	4.2445851221227775E-4	0.0	0.0	0.18530645904581727	0.0
28	4.2445851221227775E-4	0.0	0.0	0.2993645249702879	0.0
29	4.2445851221227775E-4	0.0	0.0	0.4300371098013534	0.0
30	4.2445851221227775E-4	0.0	0.0	0.7032671178054282	0.0
31	4.2445851221227775E-4	0.0	0.0	1.0806713720924592	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5635	0.0	30.860691	1
ATTGGAC	360	0.0	25.694445	3
TCCGACG	45	1.3235532E-4	24.666666	23
GTATTGG	365	0.0	24.328768	1
GAACCGT	55	1.9033101E-5	23.545454	6
AACCGTG	50	2.702794E-4	22.199999	7
CGTCTGT	570	0.0	21.421053	34
GCGCGCT	80	6.963892E-7	20.8125	23
GGATCGT	45	0.003826503	20.555555	12
AACGGTA	45	0.003826503	20.555555	18
GGACCCT	825	0.0	19.733334	6
TATTGGA	500	0.0	19.24	2
TTGGACC	905	0.0	19.01105	4
CTAAACG	80	1.6173653E-5	18.5	26
CGGGATA	90	2.1533706E-6	18.5	24
CTGTGCG	60	9.2391396E-4	18.5	9
TCGCACG	50	0.00703615	18.499998	22
GACCCTC	845	0.0	18.390533	7
ACGATCT	215	0.0	18.069769	32
TATACCG	95	3.608311E-6	17.526316	5
>>END_MODULE