FastQCFastQC Report
Wed 25 May 2016
SRR2088166_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088166_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1023687
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161791.5804635596622798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT161161.5743093347869026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT125941.2302588584205914No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64420.629293915034576No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.4924356761392887No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT43940.4292327635302588No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA43200.42200399145441914No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT40320.3938703920241246No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35750.34922784015035846No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA34190.33398880712561557No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG30220.29520742179982745No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT29240.28563418310479666No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT27780.27137201117138343No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA23770.23219988140906353No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT22970.22438499267842613No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA22880.2235058176962294No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA20080.19615370713899852No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.1939069266289403No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA19200.1875573295352974No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC17430.17026688821876218No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA17260.16860622436350176No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG15600.15239033024742915No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15600.15239033024742915No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15550.15190189970176432No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13960.13636980834962248No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA13160.1285549196189851No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC12120.11839556426915648No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC11490.11224133939377955No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11480.11214365328464657No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC11450.11185059495724767No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC11330.11067836164765207No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA11250.10989687277458832No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA10490.10247272848048279No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10420.10178892571655203No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC10370.1013004951708872No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.031.3168011
TATACCG405.939506E-527.755
GTATTGG2200.020.1818181
ATTGGAC2550.019.5882363
CGTCTGT3050.019.40983634
TGTACCG609.235871E-418.55
AGCCGTC500.007034369718.49999812
GTATCAA55850.017.5559542
ACCGGGC650.001579774517.0769238
TGTCCGT650.001579774517.07692310
TAATAGT1201.0408439E-716.9583344
GGCGCCT803.382114E-416.187519
TTGGACC5450.015.9541284
TTTTTAC7550.015.4370871
TGGACCC5650.015.389385
GTACACT1101.4514802E-515.1363644
GGACCCT5550.015.0000016
TGCGTCT3900.014.70512932
GTTATCA1904.5474735E-1014.6052641
TCAGAAC1651.6438207E-814.5757573
Produced by FastQC (version 0.11.5)