##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088166_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1023687
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.78525174198754	31.0	31.0	33.0	28.0	34.0
2	30.925772233114223	31.0	31.0	34.0	28.0	34.0
3	30.969629388670562	31.0	31.0	34.0	27.0	34.0
4	34.96117954023056	35.0	35.0	37.0	32.0	37.0
5	34.81435634134262	35.0	35.0	37.0	32.0	37.0
6	34.8963579687932	35.0	35.0	37.0	32.0	37.0
7	34.71904791210595	35.0	35.0	37.0	31.0	37.0
8	34.805812714237845	35.0	35.0	37.0	32.0	37.0
9	36.22259538315911	38.0	35.0	39.0	32.0	39.0
10	36.04211345850831	38.0	35.0	39.0	30.0	39.0
11	36.10807795742253	38.0	35.0	39.0	31.0	39.0
12	35.916643466215746	37.0	35.0	39.0	30.0	39.0
13	35.985088215440854	37.0	35.0	39.0	30.0	39.0
14	36.79718996138468	38.0	36.0	40.0	31.0	41.0
15	36.864106899862946	38.0	36.0	40.0	31.0	41.0
16	36.81830969817923	38.0	36.0	40.0	31.0	41.0
17	36.95269354793018	38.0	36.0	40.0	31.0	41.0
18	36.98061712222584	38.0	36.0	40.0	31.0	41.0
19	37.092784220176675	39.0	36.0	40.0	31.0	41.0
20	37.06961600567361	39.0	36.0	40.0	31.0	41.0
21	36.954702951195046	39.0	36.0	40.0	31.0	41.0
22	36.90749223151217	39.0	36.0	40.0	31.0	41.0
23	36.77605850225704	39.0	35.0	40.0	31.0	41.0
24	36.70561704896125	38.0	35.0	40.0	30.0	41.0
25	36.60325763636737	38.0	35.0	40.0	30.0	41.0
26	36.310546094655884	38.0	35.0	40.0	30.0	41.0
27	36.11768636311685	38.0	35.0	40.0	30.0	41.0
28	35.94486889058863	38.0	34.0	40.0	29.0	41.0
29	35.793256141769895	38.0	34.0	40.0	29.0	41.0
30	35.5820157919364	38.0	34.0	40.0	28.0	41.0
31	35.29184408906238	38.0	34.0	40.0	27.0	41.0
32	35.00631052264999	37.0	33.0	40.0	27.0	41.0
33	35.141757197268305	37.0	33.0	40.0	27.0	41.0
34	35.085807478262396	37.0	33.0	40.0	26.0	41.0
35	34.98532461582496	37.0	33.0	40.0	26.0	41.0
36	34.86374253067588	37.0	33.0	40.0	25.0	41.0
37	34.704945945391515	37.0	33.0	40.0	25.0	41.0
38	34.56460617356672	37.0	33.0	40.0	24.0	41.0
39	34.467475898394724	37.0	33.0	40.0	24.0	41.0
40	34.270043480087175	37.0	33.0	40.0	23.0	41.0
41	34.09040849400256	37.0	33.0	40.0	21.0	41.0
42	33.82033082377719	37.0	33.0	40.0	20.0	41.0
43	33.48151632286041	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	1.0
15	11.0
16	17.0
17	52.0
18	104.0
19	261.0
20	510.0
21	990.0
22	1798.0
23	3078.0
24	5204.0
25	7913.0
26	11926.0
27	16698.0
28	22840.0
29	29605.0
30	37957.0
31	45308.0
32	54591.0
33	65452.0
34	78244.0
35	92513.0
36	114683.0
37	144591.0
38	168927.0
39	120411.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23552804714722	24.35764056786889	15.236786244232855	21.170045140751032
2	18.137575254936323	24.392319136611093	35.53322451100776	21.936881097444825
3	20.778421529236965	25.99339446530043	33.074172085803575	20.154011919659037
4	13.749417546574294	16.707645989447947	34.74655827415997	34.79637818981779
5	10.723981060617161	41.65589677313476	34.37290890672637	13.24721325952171
6	31.511585084112625	36.488203913891645	14.870365648875094	17.129845353120633
7	23.51001819892213	36.68992572925123	21.131068383206976	18.668987688619666
8	27.812114445138015	34.06451386019359	20.078500557299254	18.044871137369135
9	24.869711151943903	13.878070152302413	24.802307736642156	36.44991095911153
10	16.435004058857835	28.866733679337532	33.63616027164553	21.062101990159103
11	31.042301015837847	22.021770326281374	25.384419260965508	21.551509396915268
12	19.959421190266166	28.489079181429478	31.792432647869905	19.75906698043445
13	31.22966297315488	21.24975700580353	29.589024770266693	17.931555250774895
14	20.586956755336345	23.269710370455034	29.62741541115595	26.515917463052673
15	22.8061897826191	29.72461308974325	27.535662756291718	19.933534371345928
16	21.047058329352623	28.668430877797608	28.203933428870346	22.08057736397942
17	18.674653482949378	27.966165439240704	28.877479151342158	24.481701926467757
18	19.46249195310676	26.653654876930155	34.010981872388726	19.872871297574356
19	18.91515668363474	25.27403395764526	35.64976403920339	20.16104531951661
20	23.571072017130238	24.047780229699118	34.21455972382183	18.166588029348816
21	23.10598845154818	24.82145421403222	32.63692906132441	19.435628273095194
22	20.879917396626116	24.86629213812425	33.51639710184852	20.737393363401118
23	20.16104531951661	27.024178288871504	34.403093914448455	18.41168247716343
24	18.512006111242986	25.979718410021814	32.90361213925741	22.60466333947779
25	19.72849122827583	27.02339679999844	34.290266458399884	18.95784551332585
26	21.269294227630127	25.830454035266637	32.70560239604489	20.19464934105835
27	19.953462337609054	27.272203319960102	32.32081681217013	20.453517530260715
28	19.733473219841613	27.00444569482664	32.399747188349565	20.862333896982182
29	18.619265459070984	26.826461603986374	33.26348776530326	21.290785171639378
30	17.95773512802253	26.9986822143878	35.72400548214444	19.31957717544523
31	19.678573626508882	26.223933682854234	34.16581435536448	19.9316783352724
32	20.6265196295352	26.744502958423816	32.04905405656221	20.579923355478773
33	21.754305759475308	26.764528610796074	31.29648027180183	20.18468535792679
34	19.864861036625452	29.632690461049126	31.46733327667539	19.035115225650028
35	18.40572362450632	30.50072922680468	31.55124564442061	19.542301504268394
36	19.51573088258423	26.5894751032298	33.9557892207286	19.939004793457375
37	19.335988441779566	26.958533223534147	33.23193515205332	20.473543182632973
38	20.03913305531867	26.450077025497055	33.53476209036551	19.976027828818772
39	21.079685489803033	26.024947078550376	33.28566251207645	19.60970491957014
40	19.089526388437093	26.894060391506386	32.43042062661732	21.585992593439205
41	19.925524110397024	26.460138694737743	32.353248600402274	21.261088594462958
42	17.390569578396523	28.160756168633576	32.61426588400556	21.834408368964343
43	17.921982012079866	27.912242706999308	32.43735634036576	21.728418940555073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	135.5
2	185.0
3	405.0
4	625.0
5	625.0
6	1247.5
7	1870.0
8	2161.5
9	2453.0
10	3742.0
11	5031.0
12	5031.0
13	9389.5
14	13748.0
15	23509.5
16	33271.0
17	31945.5
18	30620.0
19	30620.0
20	33463.5
21	36307.0
22	29470.5
23	22634.0
24	24137.5
25	25641.0
26	25641.0
27	27313.0
28	28985.0
29	29690.5
30	30396.0
31	32083.0
32	33770.0
33	33770.0
34	36072.5
35	38375.0
36	41298.0
37	44221.0
38	45586.0
39	46951.0
40	46951.0
41	48889.0
42	50827.0
43	57922.0
44	65017.0
45	83823.0
46	102629.0
47	102629.0
48	104503.5
49	106378.0
50	95553.5
51	84729.0
52	77228.5
53	69728.0
54	69728.0
55	59684.5
56	49641.0
57	39675.5
58	29710.0
59	25544.5
60	21379.0
61	21379.0
62	18555.0
63	15731.0
64	13457.5
65	11184.0
66	9466.5
67	7749.0
68	7749.0
69	6580.5
70	5412.0
71	4438.5
72	3465.0
73	2823.5
74	2182.0
75	2182.0
76	1701.5
77	1221.0
78	986.5
79	752.0
80	578.5
81	405.0
82	405.0
83	285.0
84	165.0
85	129.0
86	93.0
87	80.5
88	68.0
89	68.0
90	55.5
91	43.0
92	25.5
93	8.0
94	4.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1023687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.56462231992458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56089057003621	37.367190890806974
2	12.139637313554086	11.54834527840128
3	3.899268205841854	5.564016586048732
4	1.7599181901487433	3.3483937611356076
5	0.90875782084464	2.1612361264376494
6	0.5188381265974361	1.480700372207056
7	0.35976488585325306	1.1978456643706532
8	0.26076995993668894	0.9922739725416377
9	0.19539343146121496	0.8364433294122083
>10	1.2149090716737592	10.95136662255007
>50	0.09910021880037717	3.2338018879219805
>100	0.06537424219153719	6.070415348506504
>500	0.00972338409325903	3.376957587568085
>1k	0.006620176403921043	6.323898721447945
>5k	4.1376102524506516E-4	1.1299494136617612
>10k+	6.206415378675977E-4	4.417164436981869
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16179	1.5804635596622798	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16116	1.5743093347869026	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12594	1.2302588584205914	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6442	0.629293915034576	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5041	0.4924356761392887	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4394	0.4292327635302588	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4320	0.42200399145441914	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4032	0.3938703920241246	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3575	0.34922784015035846	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3419	0.33398880712561557	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3022	0.29520742179982745	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2924	0.28563418310479666	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2778	0.27137201117138343	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2377	0.23219988140906353	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2297	0.22438499267842613	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2288	0.2235058176962294	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2008	0.19615370713899852	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1985	0.1939069266289403	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1920	0.1875573295352974	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	1743	0.17026688821876218	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1726	0.16860622436350176	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1560	0.15239033024742915	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1560	0.15239033024742915	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1555	0.15190189970176432	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1396	0.13636980834962248	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	1316	0.1285549196189851	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1212	0.11839556426915648	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	1149	0.11224133939377955	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1148	0.11214365328464657	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	1145	0.11185059495724767	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	1133	0.11067836164765207	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1125	0.10989687277458832	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1049	0.10247272848048279	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1042	0.10178892571655203	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1037	0.1013004951708872	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.76861091329674E-5	0.0
10	0.0	0.0	0.0	2.930583273989022E-4	0.0
11	0.0	0.0	0.0	2.930583273989022E-4	0.0
12	0.0	0.0	0.0	4.88430545664837E-4	0.0
13	0.0	0.0	0.0	5.861166547978044E-4	0.0
14	0.0	0.0	0.0	8.791749821967066E-4	0.0
15	0.0	0.0	0.0	0.0013676055278615436	0.0
16	0.0	0.0	0.0	0.0026375249465901197	0.0
17	0.0	0.0	0.0	0.0038097582561857288	0.0
18	0.0	0.0	0.0	0.0038097582561857288	0.0
19	0.0	0.0	0.0	0.0047866193475154025	0.0
20	0.0	0.0	0.0	0.005763480438845076	0.0
21	0.0	0.0	0.0	0.008010260948903327	0.0
22	0.0	0.0	0.0	0.013969113606014338	0.0
23	0.0	0.0	0.0	0.023053921755380305	0.0
24	0.0	0.0	0.0	0.03585080205179904	0.0
25	0.0	0.0	0.0	0.042200399145441915	0.0
26	0.0	0.0	0.0	0.05187132394960569	0.0
27	0.0	0.0	0.0	0.1156603532134334	0.0
28	0.0	0.0	0.0	0.19009716837275456	0.0
29	0.0	0.0	0.0	0.27440028055450544	0.0
30	0.0	0.0	0.0	0.4513098241943094	0.0
31	0.0	0.0	0.0	0.7083219773231466	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3125	0.0	31.316801	1
TATACCG	40	5.939506E-5	27.75	5
GTATTGG	220	0.0	20.181818	1
ATTGGAC	255	0.0	19.588236	3
CGTCTGT	305	0.0	19.409836	34
TGTACCG	60	9.235871E-4	18.5	5
AGCCGTC	50	0.0070343697	18.499998	12
GTATCAA	5585	0.0	17.555954	2
ACCGGGC	65	0.0015797745	17.076923	8
TGTCCGT	65	0.0015797745	17.076923	10
TAATAGT	120	1.0408439E-7	16.958334	4
GGCGCCT	80	3.382114E-4	16.1875	19
TTGGACC	545	0.0	15.954128	4
TTTTTAC	755	0.0	15.437087	1
TGGACCC	565	0.0	15.38938	5
GTACACT	110	1.4514802E-5	15.136364	4
GGACCCT	555	0.0	15.000001	6
TGCGTCT	390	0.0	14.705129	32
GTTATCA	190	4.5474735E-10	14.605264	1
TCAGAAC	165	1.6438207E-8	14.575757	3
>>END_MODULE