FastQCFastQC Report
Wed 25 May 2016
SRR2088165_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088165_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753919
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT149221.97925771866739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT141551.8775226516376426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT89571.188058664127048No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46690.6192972985161537No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT36500.4841368900372587No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG31850.4224591766489504No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT28920.38359558520212383No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA27950.37072948154907887No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA23820.31594906084075347No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21800.28915573158389696No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG20120.2668721706177985No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT19890.2638214450093445No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.25931167671858646No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18580.2464455730655415No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT15880.21063270722716898No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.20890838405717327No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14010.18582898162799982No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT13990.18556370114030818No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11400.15120987798423968No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA11370.15081195725270222No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC11050.14656746944963583No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG11010.14603690847425255No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA10580.1403333779888821No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9740.12919159750583287No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9600.12733463409199133No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC9270.12295750604507912No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG9250.12269222555738746No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9100.1207026218997001No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA8990.11924357921739602No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8750.11606021336509624No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC8710.11552965238971295No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA8620.11433589019510053No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT8570.11367268897587142No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA8490.11261156702510483No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC8410.11155044507433823No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.10544899385743031No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA7940.10531635361358449No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC7770.10306146946820548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29500.032.610171
GTACTAG509.085672E-625.9000021
CGTTGTG400.001930574323.12535
TCACGTT753.737241E-722.224
CGTGTCT855.174843E-821.76470635
GTATCAA46100.020.907812
ACGTTTA806.953578E-720.812526
TCGTGTC909.467476E-820.55555734
CGTTTAT909.467476E-820.55555727
TAAGCCG656.898103E-519.92307736
CGACGAG500.00703269218.524
ATCACGT902.1502165E-618.523
CGTCTGT2750.018.16363734
CACGTTT953.6030597E-617.52631425
TATCCTG953.6030597E-617.5263145
GGCAGTT1301.3907993E-817.07692312
TATACAG904.4426917E-516.4444455
GCGTCTG3150.016.44444533
TCGAACC904.4426917E-516.44444531
AAGCCGT803.380635E-416.187537
Produced by FastQC (version 0.11.5)