FastQCFastQC Report
Wed 25 May 2016
SRR2088164_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088164_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2522738
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT471731.8699127693799358No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT462061.8315814008430524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT409081.6215714830473873No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG210020.8325081716769637No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148320.5879326350972633No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT126370.5009239960709356No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA123630.4900627809943006No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT113400.4495116020767912No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA100050.39659290818150755No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA98810.391677613767264No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT97050.3847010668567247No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT90040.3569137976278155No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG85630.3394327908803847No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT73600.2917465071680056No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA73450.2911519151017664No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA73060.2896059757295446No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA63730.25262234920947No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT57030.22606390358412168No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA55210.21884951984708678No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC54200.2148459332677432No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50090.19855411065279074No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG44630.17691095944168597No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.17473078853214244No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40720.16141192624838568No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39510.15661555024738993No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC38840.1539597056848551No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA38810.15384078727160727No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC36650.14527866151776364No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35760.1417507485914114No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA35610.14115615652517227No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC35340.1400858908059418No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG34470.13663725682175476No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC34260.13580482792902No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34030.13489312009411997No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31970.1267273890511024No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA31750.12585532068728503No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT30960.12272380247175885No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT30210.11975084214056314No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC30190.11967156319839793No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC29600.11733283440452397No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA28880.1144787924865761No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC28580.11328960835409782No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG28100.11138691374213255No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG27720.1098806138409934No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT27690.10976169542774557No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA27320.10829503499768901No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA27220.10789864028686291No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTG27210.10785900081578031No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT26680.10575810884840202No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTC26290.10421216947618024No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA26140.1036175774099411No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA26120.10353829846777589No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAACTGTCTCT25530.10119956967390192No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG25320.10036714078116712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100950.030.8424971
GTATTGG8150.025.6503091
ATTGGAC7500.024.6666663
TATTGGA9600.020.8124982
GAACCGT555.1454356E-420.1818186
CGGGTAA656.906311E-519.92307724
TCCACGG3250.019.9230772
GTTATCA3100.019.6935481
TTGGACC15700.019.6783454
GTCGTCA851.2465152E-619.58823624
CACGGCT3150.018.793654
CGTGCAC500.007037156318.510
GCGTCGA500.007037156318.511
TTTTTAC22700.018.4185031
ATCCACG3500.017.971431
ACGGCTC3400.017.9558835
GGACCCT16300.017.9325166
TGGACCC17350.017.593665
GTATCAA179000.017.4354762
GACCCTC16450.017.0942237
Produced by FastQC (version 0.11.5)