##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088164_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2522738
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0698792343874	31.0	31.0	33.0	28.0	34.0
2	31.196449254738305	31.0	31.0	34.0	28.0	34.0
3	31.231613033140977	31.0	31.0	34.0	28.0	34.0
4	35.21168191068593	37.0	35.0	37.0	33.0	37.0
5	35.101215425462335	35.0	35.0	37.0	32.0	37.0
6	35.16224078758872	37.0	35.0	37.0	32.0	37.0
7	34.974864611386515	37.0	35.0	37.0	32.0	37.0
8	35.0539481309593	37.0	35.0	37.0	32.0	37.0
9	36.4830327207978	38.0	35.0	39.0	32.0	39.0
10	36.36505812335645	38.0	35.0	39.0	32.0	39.0
11	36.4088109823533	38.0	35.0	39.0	32.0	39.0
12	36.24747318191584	38.0	35.0	39.0	32.0	39.0
13	36.29018907234917	38.0	35.0	39.0	32.0	39.0
14	37.14276670823526	39.0	36.0	41.0	31.0	41.0
15	37.1978370326209	39.0	36.0	41.0	32.0	41.0
16	37.174128664966396	39.0	36.0	41.0	31.0	41.0
17	37.353214245791676	39.0	36.0	41.0	32.0	41.0
18	37.393545425644675	39.0	36.0	41.0	32.0	41.0
19	37.516676325484454	39.0	36.0	41.0	32.0	41.0
20	37.47793548121129	39.0	36.0	41.0	32.0	41.0
21	37.358016567713335	39.0	36.0	41.0	31.0	41.0
22	37.32292612233216	39.0	36.0	41.0	31.0	41.0
23	37.184582782675015	39.0	36.0	41.0	31.0	41.0
24	37.108554277138566	39.0	36.0	41.0	31.0	41.0
25	37.012551045728884	39.0	35.0	40.0	31.0	41.0
26	36.70297113691553	38.0	35.0	40.0	30.0	41.0
27	36.53412799902328	38.0	35.0	40.0	30.0	41.0
28	36.35357932532035	38.0	35.0	40.0	30.0	41.0
29	36.20139269317701	38.0	35.0	40.0	30.0	41.0
30	35.99573717127978	38.0	35.0	40.0	30.0	41.0
31	35.64980707469424	38.0	34.0	40.0	29.0	41.0
32	35.327336013490104	37.0	34.0	40.0	27.0	41.0
33	35.36031644982555	38.0	34.0	40.0	27.0	41.0
34	35.277446171580245	38.0	34.0	40.0	27.0	41.0
35	35.145473291320776	38.0	34.0	40.0	26.0	41.0
36	35.007464905194276	38.0	34.0	40.0	25.0	41.0
37	34.82830163100568	38.0	33.0	40.0	25.0	41.0
38	34.649769020802005	38.0	33.0	40.0	24.0	41.0
39	34.489292586071166	38.0	33.0	40.0	23.0	41.0
40	34.226891179345614	38.0	33.0	40.0	21.0	41.0
41	34.012551838518306	38.0	33.0	40.0	20.0	41.0
42	33.702721011852994	37.0	33.0	40.0	18.0	41.0
43	33.354052620605074	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	3.0
15	12.0
16	33.0
17	94.0
18	227.0
19	535.0
20	1145.0
21	2289.0
22	3987.0
23	6756.0
24	11048.0
25	17050.0
26	25913.0
27	37752.0
28	52191.0
29	69276.0
30	86568.0
31	103693.0
32	125417.0
33	151278.0
34	181678.0
35	214616.0
36	267918.0
37	354854.0
38	401790.0
39	406613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72309530359475	23.267219980830355	15.144497763937437	20.86518695163747
2	17.070302187543852	23.536728744721014	37.08098106105351	22.31198800668163
3	20.148901709174712	26.14643296291569	33.292597170217434	20.412068157692158
4	12.567535748856995	15.2925115489599	34.29171003885461	37.8482426633285
5	10.17553150584801	42.019028531698495	33.93095121253178	13.874488749921712
6	31.14504954537491	35.20151517914266	15.191232700343832	18.4622025751386
7	23.309475657004413	37.43321740109357	20.34991346703463	18.907393474867387
8	28.840252138747662	32.88062414725588	19.85703628359346	18.422087430402996
9	25.14874711523749	13.492086772387779	25.524489661629545	35.834676450745185
10	16.620275272342987	28.67483662591993	32.744581482500365	21.960306619236718
11	30.014531830098885	21.60914054491588	26.28889722198659	22.08743040299865
12	20.55821888757374	28.648397098707832	31.684820223106797	19.10856379061163
13	31.824470079730833	21.29896168369446	30.226325524093266	16.65024271248144
14	20.654423883891234	23.40361940082561	29.992413005234788	25.949543710048367
15	22.42428662825866	29.358696781037114	28.887343830393803	19.329672760310423
16	20.762600000475672	28.739448963784586	28.62663502908348	21.87131600665626
17	17.965282165647007	27.57222509828607	28.72533731207918	25.737155423987744
18	19.14701407756176	26.56526361437454	35.18962333781788	19.098098970245818
19	18.1492489509414	24.68520314039746	37.052955954998104	20.112591953663046
20	24.100758778755463	22.88469908488317	35.36411628952353	17.65042584683784
21	23.10604589140846	24.413078171415343	33.634249771478444	18.846626165697746
22	20.64387978458326	24.07792644341188	34.69440742558284	20.583786346422023
23	19.463257777858818	26.739360171369363	36.095028496815765	17.70235355395606
24	17.476408568785185	25.682770069662407	34.059184901484024	22.781636460068384
25	19.083908039598246	27.004944627622844	35.779419028056026	18.131728304722884
26	21.098306681074295	25.37263084791207	33.74420966426161	19.784852806752028
27	19.16643741839224	27.322020756812638	33.223664130004785	20.287877694790343
28	18.811703791673967	26.819312984542986	33.20844257310906	21.16054065067399
29	17.5953666215041	26.683072122432055	34.549128764065074	21.172432491998773
30	16.73166218608512	26.73432595854187	37.78513662536498	18.74887523000803
31	18.838262237299315	26.185081447221233	35.528501176103106	19.448155139376343
32	20.5056569489182	26.432312828363468	32.945157206178365	20.116873016539966
33	21.674664590615432	26.462240629030838	32.04379527323091	19.819299507122818
34	19.056636083493412	30.316505320806204	32.08977705968674	18.53708153601365
35	17.10930742708914	31.358270260328265	32.3969036816348	19.135518630947804
36	18.466047603833612	26.3684140009783	35.53734077815453	19.628197617033557
37	18.634198240166043	26.787284291908236	34.07837040548801	20.500147062437716
38	19.256458657220847	26.139694252831646	34.82165012775801	19.782196962189495
39	20.793875543159853	25.42075316580636	34.279461442290085	19.505909848743705
40	18.158088552992822	26.696628821542305	33.31895741848737	21.826325206977497
41	19.456836183543437	25.931864505945523	33.04532614960412	21.56597316090692
42	16.102742337888436	28.097765205899307	33.80200401310005	21.997488443112207
43	16.98931874812208	27.95078204712499	33.14985543484896	21.910043769903968
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	284.0
1	387.5
2	491.0
3	1418.5
4	2346.0
5	2346.0
6	4200.5
7	6055.0
8	6934.5
9	7814.0
10	11765.0
11	15716.0
12	15716.0
13	28301.5
14	40887.0
15	69094.5
16	97302.0
17	94837.5
18	92373.0
19	92373.0
20	96033.0
21	99693.0
22	77796.0
23	55899.0
24	58770.0
25	61641.0
26	61641.0
27	63570.5
28	65500.0
29	65087.0
30	64674.0
31	68303.5
32	71933.0
33	71933.0
34	76793.0
35	81653.0
36	87970.0
37	94287.0
38	95879.0
39	97471.0
40	97471.0
41	102590.5
42	107710.0
43	131615.5
44	155521.0
45	219444.5
46	283368.0
47	283368.0
48	285920.5
49	288473.0
50	255384.5
51	222296.0
52	200286.0
53	178276.0
54	178276.0
55	151381.5
56	124487.0
57	95173.5
58	65860.0
59	54418.5
60	42977.0
61	42977.0
62	37167.0
63	31357.0
64	27030.0
65	22703.0
66	19261.0
67	15819.0
68	15819.0
69	13395.0
70	10971.0
71	9214.0
72	7457.0
73	5837.5
74	4218.0
75	4218.0
76	3340.0
77	2462.0
78	1927.0
79	1392.0
80	1059.5
81	727.0
82	727.0
83	517.0
84	307.0
85	239.5
86	172.0
87	134.0
88	96.0
89	96.0
90	74.5
91	53.0
92	32.0
93	11.0
94	7.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2522738.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.27452717890583
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.22519862457901	21.649853558495504
2	13.822208146889558	8.645660487247822
3	6.091024785877632	5.714817606399572
4	3.211503991179068	4.017530754291773
5	1.9120852045051557	2.9899780348340106
6	1.1938752918830249	2.2402731158531872
7	0.8281216468430072	1.8129379066142322
8	0.6032944253426693	1.509419832180935
9	0.43412044279969897	1.2219220428532038
>10	2.3092239606969973	13.299188364812018
>50	0.19920546914882634	4.328122337355679
>100	0.1379832023024897	8.218070726152755
>500	0.016653884597093094	3.67009570290501
>1k	0.01281068045930238	8.516213404493165
>5k	0.0015372816551162858	3.493249687091042
>10k+	0.0011529612413372142	8.67266643842011
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47173	1.8699127693799358	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	46206	1.8315814008430524	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	40908	1.6215714830473873	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	21002	0.8325081716769637	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14832	0.5879326350972633	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	12637	0.5009239960709356	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12363	0.4900627809943006	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11340	0.4495116020767912	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	10005	0.39659290818150755	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9881	0.391677613767264	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	9705	0.3847010668567247	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	9004	0.3569137976278155	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8563	0.3394327908803847	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	7360	0.2917465071680056	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7345	0.2911519151017664	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7306	0.2896059757295446	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6373	0.25262234920947	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5703	0.22606390358412168	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5521	0.21884951984708678	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5420	0.2148459332677432	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5009	0.19855411065279074	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4463	0.17691095944168597	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4408	0.17473078853214244	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4072	0.16141192624838568	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3951	0.15661555024738993	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	3884	0.1539597056848551	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	3881	0.15384078727160727	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	3665	0.14527866151776364	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3576	0.1417507485914114	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3561	0.14115615652517227	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	3534	0.1400858908059418	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3447	0.13663725682175476	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC	3426	0.13580482792902	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3403	0.13489312009411997	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3197	0.1267273890511024	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	3175	0.12585532068728503	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT	3096	0.12272380247175885	No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT	3021	0.11975084214056314	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	3019	0.11967156319839793	No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC	2960	0.11733283440452397	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	2888	0.1144787924865761	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2858	0.11328960835409782	No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG	2810	0.11138691374213255	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	2772	0.1098806138409934	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT	2769	0.10976169542774557	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	2732	0.10829503499768901	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA	2722	0.10789864028686291	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTG	2721	0.10785900081578031	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT	2668	0.10575810884840202	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTC	2629	0.10421216947618024	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2614	0.1036175774099411	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2612	0.10353829846777589	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAACTGTCTCT	2553	0.10119956967390192	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	2532	0.10036714078116712	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.963947108260945E-5	0.0	0.0	0.0	0.0
7	3.963947108260945E-5	0.0	0.0	0.0	0.0
8	3.963947108260945E-5	0.0	0.0	0.0	0.0
9	3.963947108260945E-5	0.0	0.0	7.92789421652189E-5	0.0
10	3.963947108260945E-5	3.963947108260945E-5	0.0	2.7747629757826614E-4	0.0
11	3.963947108260945E-5	3.963947108260945E-5	0.0	3.963947108260945E-4	0.0
12	3.963947108260945E-5	7.92789421652189E-5	0.0	9.513473059826268E-4	0.0
13	1.1891841324782835E-4	7.92789421652189E-5	0.0	0.001070265719230455	0.0
14	1.1891841324782835E-4	7.92789421652189E-5	0.0	0.0012288236035608929	0.0
15	1.585578843304378E-4	7.92789421652189E-5	0.0	0.001942334083047863	0.0
16	1.585578843304378E-4	7.92789421652189E-5	0.0	0.0031315182155261466	0.0
17	1.585578843304378E-4	7.92789421652189E-5	0.0	0.0049152944142435715	0.0
18	1.585578843304378E-4	7.92789421652189E-5	0.0	0.005708083835895761	0.0
19	1.585578843304378E-4	7.92789421652189E-5	0.0	0.007016186381621873	0.0
20	1.585578843304378E-4	7.92789421652189E-5	0.0	0.00876032310925669	0.0
21	1.585578843304378E-4	7.92789421652189E-5	0.0	0.012565712333187196	0.0
22	1.585578843304378E-4	7.92789421652189E-5	0.0	0.02017649078104821	0.0
23	1.585578843304378E-4	7.92789421652189E-5	0.0	0.032623284700987576	0.0
24	1.585578843304378E-4	7.92789421652189E-5	0.0	0.04970789673759225	0.0
25	1.585578843304378E-4	7.92789421652189E-5	0.0	0.058864614557675034	0.0
26	1.585578843304378E-4	7.92789421652189E-5	0.0	0.07071681641137526	0.0
27	1.585578843304378E-4	7.92789421652189E-5	0.0	0.16997405200222931	0.0
28	1.585578843304378E-4	7.92789421652189E-5	0.0	0.27771413440476184	0.0
29	1.585578843304378E-4	7.92789421652189E-5	0.0	0.40115144735600766	0.0
30	1.9819735541304725E-4	7.92789421652189E-5	0.0	0.651910741424595	0.0
31	2.378368264956567E-4	7.92789421652189E-5	0.0	0.9859525642377449	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10095	0.0	30.842497	1
GTATTGG	815	0.0	25.650309	1
ATTGGAC	750	0.0	24.666666	3
TATTGGA	960	0.0	20.812498	2
GAACCGT	55	5.1454356E-4	20.181818	6
CGGGTAA	65	6.906311E-5	19.923077	24
TCCACGG	325	0.0	19.923077	2
GTTATCA	310	0.0	19.693548	1
TTGGACC	1570	0.0	19.678345	4
GTCGTCA	85	1.2465152E-6	19.588236	24
CACGGCT	315	0.0	18.79365	4
CGTGCAC	50	0.0070371563	18.5	10
GCGTCGA	50	0.0070371563	18.5	11
TTTTTAC	2270	0.0	18.418503	1
ATCCACG	350	0.0	17.97143	1
ACGGCTC	340	0.0	17.955883	5
GGACCCT	1630	0.0	17.932516	6
TGGACCC	1735	0.0	17.59366	5
GTATCAA	17900	0.0	17.435476	2
GACCCTC	1645	0.0	17.094223	7
>>END_MODULE