FastQCFastQC Report
Wed 25 May 2016
SRR2088163_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088163_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2800882
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT342121.2214723790577398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT339481.2120467766939127No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT280431.0012203298818014No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG143890.5137310318678188No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119090.42518749451065774No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA85730.3060821555495733No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT85000.3034758336838182No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT83170.29694217749980184No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA78690.2809472159127018No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA69140.24685081342234338No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG62420.2228583710416933No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT60260.21514651456219863No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT60140.2147180780911156No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47920.1710889641191596No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45660.16302007724709572No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA43780.1563079058667948No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT42610.1521306502737352No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41780.14916729801541087No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34280.12239001857272103No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA33710.1203549453350766No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32860.11732018699823842No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32520.11610628366350315No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30160.10768036639887008No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG29960.10696630561373167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74150.029.3654751
GTATTGG8150.019.5214711
AACACCG902.1544638E-618.55
ATTGGAC9150.018.1967223
GTATCAA126950.017.2248922
GGACCCT14000.016.5178576
CGTCTGT6700.016.29104434
TTGGACC14900.016.26514
TAACGCG700.002593740815.8571425
GACCCTC14800.015.6257
CGAATTA2250.015.62222215
CGATTAG855.36757E-415.2352941
CGCCGGA1701.4897523E-915.23529414
CCGTTAG1101.4532705E-515.1363649
TGGACCC15100.015.0695375
TGCGTCT7450.014.89932832
TATTGGA10900.014.2568812
ATTACGT1956.7484507E-1014.2307717
TCCGTTA1051.6569717E-414.0952388
TTAGTAC1601.7861021E-713.8753
Produced by FastQC (version 0.11.5)