##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088163_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2800882
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.869964889631195	31.0	31.0	33.0	28.0	34.0
2	31.013803509037512	31.0	31.0	34.0	28.0	34.0
3	31.07851812393382	31.0	31.0	34.0	28.0	34.0
4	35.04939729699431	35.0	35.0	37.0	32.0	37.0
5	34.89270522642511	35.0	35.0	37.0	32.0	37.0
6	34.96313554087605	36.0	35.0	37.0	32.0	37.0
7	34.80133757866272	35.0	35.0	37.0	32.0	37.0
8	34.86922333750583	36.0	35.0	37.0	32.0	37.0
9	36.34107684650763	38.0	35.0	39.0	32.0	39.0
10	36.14602685868237	38.0	35.0	39.0	31.0	39.0
11	36.194237743682166	38.0	35.0	39.0	32.0	39.0
12	36.0156682787779	38.0	35.0	39.0	30.0	39.0
13	36.086029686363084	38.0	35.0	39.0	31.0	39.0
14	36.96319159464769	39.0	36.0	40.0	31.0	41.0
15	37.03156077264233	39.0	36.0	40.0	31.0	41.0
16	36.999470166897424	39.0	36.0	40.0	31.0	41.0
17	37.07278992831544	39.0	36.0	40.0	31.0	41.0
18	37.079341436019085	39.0	36.0	40.0	31.0	41.0
19	37.17422833236102	39.0	36.0	40.0	31.0	41.0
20	37.13654484551652	39.0	36.0	40.0	31.0	41.0
21	37.02718465112061	39.0	36.0	40.0	31.0	41.0
22	36.97269395854592	39.0	36.0	40.0	31.0	41.0
23	36.85949818664264	39.0	35.0	40.0	31.0	41.0
24	36.79401524234152	39.0	35.0	40.0	30.0	41.0
25	36.690793114454664	38.0	35.0	40.0	30.0	41.0
26	36.420922409441026	38.0	35.0	40.0	30.0	41.0
27	36.24068989696817	38.0	35.0	40.0	30.0	41.0
28	36.09890884371423	38.0	35.0	40.0	29.0	41.0
29	35.94706881618005	38.0	34.0	40.0	29.0	41.0
30	35.76462985588111	38.0	34.0	40.0	29.0	41.0
31	35.517996116937454	38.0	34.0	40.0	27.0	41.0
32	35.251747842286825	38.0	34.0	40.0	27.0	41.0
33	35.43536607397241	38.0	34.0	40.0	27.0	41.0
34	35.4164120444917	38.0	34.0	40.0	27.0	41.0
35	35.33358813402349	38.0	34.0	40.0	27.0	41.0
36	35.24400635228474	38.0	34.0	40.0	26.0	41.0
37	35.105891287101706	38.0	34.0	40.0	26.0	41.0
38	34.98459628074299	38.0	33.0	40.0	25.0	41.0
39	34.94255273874444	38.0	33.0	40.0	25.0	41.0
40	34.799181115091606	38.0	33.0	40.0	24.0	41.0
41	34.67014140545728	38.0	33.0	40.0	24.0	41.0
42	34.46550265237879	38.0	33.0	40.0	23.0	41.0
43	34.14524567618343	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	5.0
15	12.0
16	30.0
17	94.0
18	219.0
19	583.0
20	1233.0
21	2512.0
22	4523.0
23	7839.0
24	12728.0
25	19668.0
26	29205.0
27	41556.0
28	57569.0
29	76156.0
30	97079.0
31	117656.0
32	142515.0
33	171496.0
34	204980.0
35	244989.0
36	305269.0
37	391036.0
38	487785.0
39	384143.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.87468947281606	23.688431001377424	14.257330369505034	22.17954915630148
2	17.97198168291274	23.97523351572826	35.83664002981918	22.216144771539824
3	20.22702134541905	26.56059769743959	32.3574145572716	20.854966399869756
4	13.5314518783726	16.749331103559523	36.1029847026758	33.61623231539208
5	11.099146625955681	41.00172731303925	35.0009032868932	12.89822277411187
6	30.986703474119935	37.43692165539284	15.542461267557862	16.03391360292936
7	24.142573660725443	35.52455976367444	22.04944728124926	18.28341929435085
8	27.137451702713644	34.48495866659145	20.411855979652124	17.96573365104278
9	25.16660823269242	13.672800210790745	23.868659943546355	37.29193161297049
10	15.939300548898524	28.241211161341322	34.35956959272115	21.459918697039004
11	31.603509180322487	22.250669610501266	25.075529779548013	21.070291429628238
12	19.75502716644257	27.932379871768966	32.10417289982227	20.208420061966194
13	30.55858833039021	21.246021788850797	28.88904280865813	19.30634707210086
14	20.515501902614965	22.73487422890361	29.265138624190524	27.48448524429091
15	22.84241178314545	29.3970970572841	26.970897024580115	20.789594134990335
16	21.3987593907919	28.616414400892292	27.68774264678055	22.29708356153526
17	19.232477483878295	27.692526854040977	28.83398872212396	24.241006939956772
18	20.22495056914215	26.780778340537015	32.61319113050817	20.38107995981266
19	19.68329976057542	25.428597134759695	34.078158237298105	20.80994486736678
20	23.42162218901046	24.569796228473745	32.814699084074235	19.19388249844156
21	22.978725987028373	25.23555080149753	31.652350938025954	20.13337227344815
22	21.431177750437183	25.124764270683308	32.43464023118432	21.009417747695192
23	20.700193724691008	26.653354193429067	33.189366778036344	19.457085303843574
24	19.54602157463256	26.07714284286164	31.874102514850676	22.502733067655118
25	20.53717364744391	26.860110493765895	32.802738565923164	19.799977292867034
26	21.555674248326063	26.154939765402467	31.51310908492396	20.776276901347504
27	20.55020525677269	27.39876224703504	31.265080071206143	20.785952424986128
28	20.423316655253597	26.8359038331497	31.441453085135322	21.29932642646138
29	19.620748035797295	26.71015772888683	32.319033790070414	21.35006044524546
30	19.166141236938934	26.890815107526844	33.94284371851438	20.00019993701984
31	20.25726181966966	26.39047271538037	32.868075127763326	20.484190337186643
32	21.011774148286147	26.70526641250863	31.27689777720018	21.00606166200504
33	21.86007836103056	26.623470749571027	30.72607128754442	20.790379601853985
34	20.495186873277774	28.793251554331817	30.869704614475012	19.8418569579154
35	19.35397492646959	29.551619811188047	31.050004962722454	20.044400299619905
36	20.418925181424992	26.375691657128	32.61729698002272	20.58808618142428
37	20.223700962768156	26.602977205037554	32.05154661995757	21.121775212236717
38	20.544457067452324	26.33816776286898	32.422751119111766	20.69462405056693
39	21.213746241362543	25.96053671664854	32.43228383059336	20.393433211395553
40	19.653130692403323	26.65617473353037	31.862499027092177	21.828195546974133
41	20.39325469619927	26.1842519606324	31.64135440193482	21.78113894123351
42	18.50866976902276	27.68692147687764	31.74450048234806	22.059908271751542
43	18.94017670148189	27.344850657757092	31.58812117040275	22.126851470358268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	233.0
1	348.5
2	464.0
3	1103.5
4	1743.0
5	1743.0
6	3270.0
7	4797.0
8	5250.0
9	5703.0
10	8897.5
11	12092.0
12	12092.0
13	22140.5
14	32189.0
15	52156.0
16	72123.0
17	69838.0
18	67553.0
19	67553.0
20	74181.5
21	80810.0
22	67587.5
23	54365.0
24	59745.5
25	65126.0
26	65126.0
27	70497.0
28	75868.0
29	80589.0
30	85310.0
31	92786.0
32	100262.0
33	100262.0
34	107961.0
35	115660.0
36	126904.0
37	138148.0
38	141129.0
39	144110.0
40	144110.0
41	150556.5
42	157003.0
43	173549.0
44	190095.0
45	227452.5
46	264810.0
47	264810.0
48	267903.5
49	270997.0
50	244885.0
51	218773.0
52	202163.5
53	185554.0
54	185554.0
55	161037.0
56	136520.0
57	113723.5
58	90927.0
59	79690.5
60	68454.0
61	68454.0
62	60032.5
63	51611.0
64	44711.0
65	37811.0
66	31891.0
67	25971.0
68	25971.0
69	21782.5
70	17594.0
71	14735.5
72	11877.0
73	9560.5
74	7244.0
75	7244.0
76	5616.5
77	3989.0
78	3146.0
79	2303.0
80	1822.5
81	1342.0
82	1342.0
83	1002.0
84	662.0
85	516.5
86	371.0
87	308.0
88	245.0
89	245.0
90	189.0
91	133.0
92	79.0
93	25.0
94	18.5
95	12.0
96	12.0
97	7.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2800882.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.934436087655534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.17188627805722	31.845281109061013
2	13.670494740047653	11.738699654064142
3	5.041036755873447	6.493022112317126
4	2.391263508582013	4.106702011118294
5	1.2547681805311508	2.6936382125919254
6	0.7733723473046531	1.992258337038706
7	0.4611077756808057	1.3858181627141988
8	0.3309165748983393	1.1366173228254899
9	0.23297103396354607	0.9002231971184607
>10	1.405973435697961	11.655659177886635
>50	0.1511897870687278	4.5607078288309815
>100	0.10139679460825612	8.149676490407334
>500	0.00693675692009179	2.046062879425532
>1k	0.005599550766820136	4.802458105899918
>5k	6.686030766352402E-4	2.097518556616056
>10k+	4.178769228970251E-4	4.395656842084055
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34212	1.2214723790577398	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33948	1.2120467766939127	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28043	1.0012203298818014	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14389	0.5137310318678188	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11909	0.42518749451065774	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8573	0.3060821555495733	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8500	0.3034758336838182	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8317	0.29694217749980184	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7869	0.2809472159127018	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6914	0.24685081342234338	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6242	0.2228583710416933	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6026	0.21514651456219863	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6014	0.2147180780911156	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4792	0.1710889641191596	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4566	0.16302007724709572	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4378	0.1563079058667948	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4261	0.1521306502737352	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4178	0.14916729801541087	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3428	0.12239001857272103	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3371	0.1203549453350766	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3286	0.11732018699823842	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3252	0.11610628366350315	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3016	0.10768036639887008	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2996	0.10696630561373167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.5703039256919784E-5	0.0
3	0.0	0.0	0.0	3.5703039256919784E-5	0.0
4	0.0	0.0	0.0	7.140607851383957E-5	0.0
5	0.0	0.0	0.0	7.140607851383957E-5	0.0
6	0.0	0.0	0.0	7.140607851383957E-5	0.0
7	0.0	0.0	0.0	1.0710911777075935E-4	0.0
8	0.0	0.0	0.0	1.0710911777075935E-4	0.0
9	0.0	0.0	0.0	2.142182355415187E-4	0.0
10	0.0	0.0	0.0	3.213273533122781E-4	0.0
11	0.0	0.0	0.0	3.9273343182611765E-4	0.0
12	0.0	0.0	0.0	6.783577458814759E-4	0.0
13	0.0	0.0	0.0	7.854668636522353E-4	0.0
14	0.0	0.0	0.0	0.0011067942169645133	0.0
15	0.0	0.0	0.0	0.0014638246095337111	0.0
16	0.0	0.0	0.0	0.0022135884339290267	0.0
17	0.0	0.0	0.0	0.0031061644153520212	0.0
18	0.0	0.0	0.0	0.0035346008864350588	0.0
19	0.0	0.0	0.0	0.004070146475288855	0.0
20	0.0	0.0	0.0	0.00492701941745493	0.0
21	0.0	0.0	0.0	0.007033498733613198	0.0
22	0.0	0.0	0.0	0.011996221190325047	0.0
23	0.0	0.0	0.0	0.01974378070907664	0.0
24	0.0	0.0	0.0	0.03177570493865861	0.0
25	0.0	0.0	0.0	0.0360957726887459	0.0
26	0.0	0.0	0.0	0.04284364710830374	0.0
27	0.0	0.0	0.0	0.09632679991516958	0.0
28	0.0	0.0	0.0	0.1588071186147792	0.0
29	0.0	0.0	0.0	0.2355329499778998	0.0
30	0.0	0.0	0.0	0.3754888638650254	0.0
31	0.0	0.0	0.0	0.6043453455018812	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7415	0.0	29.365475	1
GTATTGG	815	0.0	19.521471	1
AACACCG	90	2.1544638E-6	18.5	5
ATTGGAC	915	0.0	18.196722	3
GTATCAA	12695	0.0	17.224892	2
GGACCCT	1400	0.0	16.517857	6
CGTCTGT	670	0.0	16.291044	34
TTGGACC	1490	0.0	16.2651	4
TAACGCG	70	0.0025937408	15.857142	5
GACCCTC	1480	0.0	15.625	7
CGAATTA	225	0.0	15.622222	15
CGATTAG	85	5.36757E-4	15.235294	1
CGCCGGA	170	1.4897523E-9	15.235294	14
CCGTTAG	110	1.4532705E-5	15.136364	9
TGGACCC	1510	0.0	15.069537	5
TGCGTCT	745	0.0	14.899328	32
TATTGGA	1090	0.0	14.256881	2
ATTACGT	195	6.7484507E-10	14.23077	17
TCCGTTA	105	1.6569717E-4	14.095238	8
TTAGTAC	160	1.7861021E-7	13.875	3
>>END_MODULE