FastQCFastQC Report
Wed 25 May 2016
SRR2088162_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088162_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3127195
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT419941.3428647717842987No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT414331.3249253724184133No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT380281.2160418522030128No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG233340.7461638944805169No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139990.44765356813374285No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA130810.4182981873532031No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT121180.38750381731871536No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA118980.3804687587438583No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT115320.36876497947841436No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA101210.32364467198239955No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT94700.3028272941086181No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT92950.29723122478770914No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG85210.2724806096198031No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA72420.23158133726870248No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA69660.22275553651115457No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA69250.221444457413113No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT67340.2153367474685781No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA53570.17130367629776846No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC52670.16842569778987238No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50630.16190227983864133No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC45460.14536989218772733No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA43630.13951800255500535No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG43330.13855867638570668No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG41820.13373006800023662No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39930.12768631313365492No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34790.11124985809967079No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG32770.10479039522639298No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC32180.10290372042677223No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.10124088839998785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91100.029.9736581
GTATTGG11000.025.059091
ATTGGAC11400.023.2061423
TTGGACC20300.021.4162564
GGACCCT20900.020.0933026
GACCCTC21200.019.2853787
TATTGGA14600.019.006852
CGCCGGA2850.018.82456214
TGGACCC23000.018.821745
ACGTTTA2400.018.526
GCCGGAA3050.017.59016215
ACCGTCC852.7255428E-517.4117648
CGTCTGT10100.017.21782334
GTTATCA4200.016.7380961
GTATCAA167650.016.3095742
TATACCG3250.015.9384615
ACCCTCG25800.015.7034888
TTTTTAC23400.015.6538461
CTAGTAC3950.015.4556983
CGTGTCT3200.015.0312535
Produced by FastQC (version 0.11.5)