##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088162_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3127195
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.088537491266134	31.0	31.0	33.0	28.0	34.0
2	31.23181861060791	31.0	31.0	34.0	28.0	34.0
3	31.284997257926033	31.0	31.0	34.0	28.0	34.0
4	35.254762814599026	37.0	35.0	37.0	33.0	37.0
5	35.12873901371677	37.0	35.0	37.0	32.0	37.0
6	35.17488132335847	37.0	35.0	37.0	32.0	37.0
7	35.00055449052586	37.0	35.0	37.0	32.0	37.0
8	35.064040777757704	37.0	35.0	37.0	32.0	37.0
9	36.54753988798268	39.0	35.0	39.0	32.0	39.0
10	36.41622156597206	38.0	35.0	39.0	32.0	39.0
11	36.43568597417174	38.0	35.0	39.0	32.0	39.0
12	36.26903758799819	38.0	35.0	39.0	32.0	39.0
13	36.31248163290105	38.0	35.0	39.0	32.0	39.0
14	37.22290455184279	39.0	36.0	41.0	32.0	41.0
15	37.28495312892225	39.0	36.0	41.0	32.0	41.0
16	37.273884423580874	39.0	36.0	41.0	32.0	41.0
17	37.38190455024391	39.0	36.0	41.0	32.0	41.0
18	37.396474156552436	39.0	36.0	41.0	32.0	41.0
19	37.49765172942525	39.0	36.0	41.0	32.0	41.0
20	37.45534224760528	39.0	36.0	41.0	32.0	41.0
21	37.33520327322089	39.0	36.0	41.0	31.0	41.0
22	37.29463688705054	39.0	36.0	41.0	31.0	41.0
23	37.17140536487172	39.0	36.0	41.0	31.0	41.0
24	37.10270354103278	39.0	36.0	41.0	31.0	41.0
25	37.01410273423947	39.0	36.0	40.0	31.0	41.0
26	36.740610994837226	39.0	35.0	40.0	30.0	41.0
27	36.58987878913851	38.0	35.0	40.0	30.0	41.0
28	36.443107321417436	38.0	35.0	40.0	30.0	41.0
29	36.30140205519643	38.0	35.0	40.0	30.0	41.0
30	36.12649962666223	38.0	35.0	40.0	30.0	41.0
31	35.84904331197767	38.0	34.0	40.0	29.0	41.0
32	35.55856702252338	38.0	34.0	40.0	28.0	41.0
33	35.69639213416496	38.0	34.0	40.0	29.0	41.0
34	35.672942045507234	38.0	34.0	40.0	28.0	41.0
35	35.58860032713022	38.0	34.0	40.0	27.0	41.0
36	35.494810205311786	38.0	34.0	40.0	27.0	41.0
37	35.351216345638825	38.0	34.0	40.0	26.0	41.0
38	35.22400521873436	38.0	34.0	40.0	26.0	41.0
39	35.15708550314259	38.0	34.0	40.0	25.0	41.0
40	34.98547260404292	38.0	34.0	40.0	24.0	41.0
41	34.83676009970597	38.0	33.0	40.0	24.0	41.0
42	34.60115183095394	38.0	33.0	40.0	23.0	41.0
43	34.29031160512856	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	7.0
15	9.0
16	32.0
17	87.0
18	233.0
19	503.0
20	1033.0
21	2198.0
22	4055.0
23	7045.0
24	11641.0
25	18640.0
26	28632.0
27	41544.0
28	58263.0
29	77871.0
30	99845.0
31	122797.0
32	149286.0
33	181172.0
34	219048.0
35	263777.0
36	329864.0
37	434707.0
38	530526.0
39	544375.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.979408063776	22.58931726355408	14.105388375205255	22.325886297464663
2	17.186648098375702	23.026993839527115	37.104561755822715	22.681796306274475
3	19.581062261867263	26.553540792947032	32.42723910725107	21.438157837934636
4	12.565797783636773	15.568968356626305	35.66790686221998	36.197326997516946
5	10.903733217787826	40.86486451916174	34.8264818791281	13.404920383922333
6	31.31934529186699	36.216513520902915	15.577538337072042	16.88660285015805
7	24.363591013671996	35.59704463584778	21.444713233424842	18.59465111705538
8	27.97881168267409	33.448122039079756	20.233531967146277	18.339534311099882
9	25.27654975145458	13.633559787605186	24.045350545776646	37.04453991516359
10	16.254151084278405	27.72222390992567	33.74755971405685	22.276065291739084
11	31.16588508231818	21.82879545407306	25.68010629333956	21.3252131702692
12	20.428786820137535	27.37053493626077	32.07574199882003	20.124936244781665
13	31.364881307369703	20.936398273852447	29.022878330260827	18.675842088517026
14	20.94714272694859	22.399306726954986	28.851382788729197	27.802167757367226
15	22.694875119715913	28.905264941904807	27.536370453393538	20.863489484985745
16	21.546529717526408	28.4888854068902	27.400849643210606	22.563735232372782
17	19.08221265383195	27.06140806697376	28.14535070566434	25.711028573529955
18	20.270593934820184	26.566395763615635	32.9554760736059	20.20753422795828
19	19.49104548964807	24.663380441577836	34.48090061540774	21.364673453366358
20	24.426011169754364	23.49386590858581	32.89913164992909	19.180991271730736
21	23.614453208066656	24.64403403049698	31.63346065723436	20.10805210420201
22	21.73430182639714	24.177353826672146	32.6111739114446	21.477170435486116
23	20.672135891749637	26.306866057281365	33.756033761885654	19.26496428908335
24	19.03104859146935	25.463458466772938	32.08140202321889	23.424090918538816
25	20.583014490621785	26.479768610527966	33.30809239590112	19.62912450294913
26	22.190653285132523	25.20690267156349	31.74582972919821	20.856614314105773
27	20.467223821987435	26.88070299421686	31.39510008170261	21.25697310209309
28	20.21424951114337	26.331776560144153	31.443641985869125	22.010331942843347
29	19.295374928650116	26.251417004695902	32.46868199776477	21.984526068889213
30	18.502555804802707	26.56418931342625	34.71900537062767	20.21424951114337
31	20.327481976659595	25.86058752332362	33.280367869608384	20.531562630408402
32	21.525296631645933	26.00256779637982	31.130901654677757	21.34123391729649
33	22.55929035445503	25.981814373583994	30.49035957143702	20.96853570052395
34	20.389326537040382	29.0509546094823	30.663166192066694	19.896552661410624
35	18.899173220729757	30.066785090152674	30.927588461864385	20.106453227253176
36	20.098938505593672	25.999785750488858	33.226581649049706	20.674694094867764
37	20.12250595181944	26.409705822630187	32.21903335097427	21.248754874576097
38	20.57230201506462	25.920097723359113	32.83926969696485	20.668330564611416
39	21.52229074298213	25.368868906480092	32.71932194826354	20.38951840227424
40	19.55013998167687	26.269484314217696	31.793796037663146	22.38657966644229
41	20.724195325203574	25.670640941802482	31.592401497188376	22.012762235805567
42	17.918454077855714	27.68557765025846	32.11871981120461	22.277248460681218
43	18.776955066761104	27.342586567195205	31.614306111387364	22.26615225465633
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	218.0
1	325.5
2	433.0
3	1409.0
4	2385.0
5	2385.0
6	3991.5
7	5598.0
8	6171.0
9	6744.0
10	10340.0
11	13936.0
12	13936.0
13	25375.0
14	36814.0
15	61427.5
16	86041.0
17	84582.0
18	83123.0
19	83123.0
20	86691.5
21	90260.0
22	72630.0
23	55000.0
24	60224.5
25	65449.0
26	65449.0
27	70841.5
28	76234.0
29	78733.5
30	81233.0
31	87080.5
32	92928.0
33	92928.0
34	102258.0
35	111588.0
36	121832.5
37	132077.0
38	138207.5
39	144338.0
40	144338.0
41	153608.5
42	162879.0
43	187046.5
44	211214.0
45	271242.0
46	331270.0
47	331270.0
48	334555.5
49	337841.0
50	303639.5
51	269438.0
52	248718.0
53	227998.0
54	227998.0
55	196134.0
56	164270.0
57	131675.0
58	99080.0
59	85287.5
60	71495.0
61	71495.0
62	62246.0
63	52997.0
64	46324.0
65	39651.0
66	33351.5
67	27052.0
68	27052.0
69	22619.0
70	18186.0
71	15065.5
72	11945.0
73	9843.5
74	7742.0
75	7742.0
76	6040.5
77	4339.0
78	3449.0
79	2559.0
80	1965.0
81	1371.0
82	1371.0
83	1027.5
84	684.0
85	531.0
86	378.0
87	304.0
88	230.0
89	230.0
90	179.0
91	128.0
92	82.0
93	36.0
94	24.5
95	13.0
96	13.0
97	6.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3127195.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.20834189146778
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.47439428014705	26.594436988612806
2	13.998972043190038	10.417570758242286
3	5.598152671233492	6.248939356556682
4	2.8893837884446194	4.3003671942444734
5	1.5843627120190817	2.9475754734449557
6	0.966807927836872	2.158399195340151
7	0.6301562338601527	1.6412948016155802
8	0.437121762525321	1.3011660790594508
9	0.3148706774003404	1.0544234234678933
>10	1.7802134700665415	12.601596262199658
>50	0.18775688775721983	4.860007444681645
>100	0.11846494235711799	8.076792572892593
>500	0.009888146908072278	2.5005433190041675
>1k	0.00771969363875506	5.9898273951279
>5k	8.673813077252876E-4	2.2862774263761785
>10k+	8.673813077252876E-4	7.020782309133555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41994	1.3428647717842987	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41433	1.3249253724184133	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38028	1.2160418522030128	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23334	0.7461638944805169	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13999	0.44765356813374285	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	13081	0.4182981873532031	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	12118	0.38750381731871536	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11898	0.3804687587438583	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11532	0.36876497947841436	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	10121	0.32364467198239955	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	9470	0.3028272941086181	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	9295	0.29723122478770914	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8521	0.2724806096198031	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7242	0.23158133726870248	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6966	0.22275553651115457	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6925	0.221444457413113	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6734	0.2153367474685781	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5357	0.17130367629776846	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5267	0.16842569778987238	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5063	0.16190227983864133	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4546	0.14536989218772733	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4363	0.13951800255500535	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4333	0.13855867638570668	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4182	0.13373006800023662	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3993	0.12768631313365492	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3479	0.11124985809967079	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3277	0.10479039522639298	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3218	0.10290372042677223	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3166	0.10124088839998785	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.197753897662282E-5	0.0
8	0.0	0.0	0.0	6.395507795324564E-5	0.0
9	2.2384277283635974E-4	0.0	0.0	9.593261692986845E-5	0.0
10	2.2384277283635974E-4	0.0	0.0	3.837304677194738E-4	0.0
11	2.558203118129826E-4	0.0	0.0	6.715283185090793E-4	0.0
12	2.558203118129826E-4	0.0	0.0	9.273486303220618E-4	0.0
13	2.558203118129826E-4	0.0	0.0	0.001055258786228553	0.0
14	2.558203118129826E-4	0.0	0.0	0.001407011714971404	0.0
15	2.558203118129826E-4	0.0	0.0	0.002014584955527238	0.0
16	2.558203118129826E-4	0.0	0.0	0.0032936865145921507	0.0
17	2.558203118129826E-4	0.0	0.0	0.0047006982295635545	0.0
18	2.558203118129826E-4	0.0	0.0	0.005212338853189519	0.0
19	3.51752928742851E-4	0.0	0.0	0.006203642561464827	0.0
20	3.51752928742851E-4	0.0	0.0	0.007642631815412854	0.0
21	3.51752928742851E-4	0.0	0.0	0.01100027340795825	0.0
22	3.51752928742851E-4	0.0	0.0	0.01790742182690878	0.0
23	3.51752928742851E-4	0.0	0.0	0.02967515617030598	0.0
24	3.51752928742851E-4	0.0	0.0	0.04659127428893945	0.0
25	3.51752928742851E-4	0.0	0.0	0.05317864731812375	0.0
26	3.51752928742851E-4	0.0	0.0	0.06206840315362489	0.0
27	3.837304677194738E-4	0.0	0.0	0.133953910773073	0.0
28	3.837304677194738E-4	0.0	0.0	0.20887728459530025	0.0
29	4.476855456727195E-4	0.0	0.0	0.30260345133578176	0.0
30	4.476855456727195E-4	0.0	0.0	0.4858027721328539	0.0
31	4.476855456727195E-4	0.0	0.0	0.7618648661180387	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9110	0.0	29.973658	1
GTATTGG	1100	0.0	25.05909	1
ATTGGAC	1140	0.0	23.206142	3
TTGGACC	2030	0.0	21.416256	4
GGACCCT	2090	0.0	20.093302	6
GACCCTC	2120	0.0	19.285378	7
TATTGGA	1460	0.0	19.00685	2
CGCCGGA	285	0.0	18.824562	14
TGGACCC	2300	0.0	18.82174	5
ACGTTTA	240	0.0	18.5	26
GCCGGAA	305	0.0	17.590162	15
ACCGTCC	85	2.7255428E-5	17.411764	8
CGTCTGT	1010	0.0	17.217823	34
GTTATCA	420	0.0	16.738096	1
GTATCAA	16765	0.0	16.309574	2
TATACCG	325	0.0	15.938461	5
ACCCTCG	2580	0.0	15.703488	8
TTTTTAC	2340	0.0	15.653846	1
CTAGTAC	395	0.0	15.455698	3
CGTGTCT	320	0.0	15.03125	35
>>END_MODULE