FastQCFastQC Report
Wed 25 May 2016
SRR2088161_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088161_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640349
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT344541.304903253319921No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT337861.2796035675586825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT301051.1401901794043137No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG196260.7433108274701564No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117590.4453577917161709No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA110580.4188082711793024No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT102070.38657768348047933No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT97380.36881488015410085No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA95490.36165673553003785No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA82320.31177696584807535No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT77850.29484738570545027No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT77590.29386266739737815No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG74430.2818945525761935No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA63250.23955166532909095No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA60880.2305755792132025No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA58620.2220161046891907No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT54800.20754832031674603No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42270.1600924726238842No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42210.15986522993740598No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC41590.15751705551046472No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG37650.14259478576506363No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37150.14070109671107872No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36320.13755757288146378No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35490.1344140490518488No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT33640.12740739955210467No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28940.10960672244464652No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG27680.10483462602860455No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27670.10479675224752485No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC26530.10047914120443927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78550.028.7568421
ATTGGAC9250.025.43
GTATTGG11000.023.3772741
TCGCTAA450.003827102520.55555714
TTGGACC16150.020.5046444
TATTGGA12350.019.9230772
GGACCCT16500.019.0606046
TGGACCC17300.019.0346815
TTGGACG609.2411425E-418.54
TGCGACG609.2411425E-418.522
CGTCTGT8300.017.83132634
ATCACGT953.6099718E-617.52631623
TAATACG650.001580667817.0769234
GACCCTC18400.016.7907627
GTATCAA136200.016.6391332
GCCGATA1251.6599915E-716.2799992
ATGCGCT1251.6599915E-716.27999935
CGACGAG1151.2436121E-616.08695624
ACGCACC700.002593688715.85714224
TATACCG1403.4784534E-815.8571425
Produced by FastQC (version 0.11.5)