##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2640349
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.126688933925024	31.0	31.0	33.0	30.0	34.0
2	31.27017413228327	31.0	31.0	34.0	28.0	34.0
3	31.318724910987147	31.0	31.0	34.0	28.0	34.0
4	35.27759284852116	37.0	35.0	37.0	33.0	37.0
5	35.15668799844263	37.0	35.0	37.0	32.0	37.0
6	35.203323878775116	37.0	35.0	37.0	32.0	37.0
7	35.03693451130892	37.0	35.0	37.0	32.0	37.0
8	35.09993186506784	37.0	35.0	37.0	32.0	37.0
9	36.59431613017824	39.0	35.0	39.0	32.0	39.0
10	36.44769574022222	38.0	35.0	39.0	32.0	39.0
11	36.480451258526806	38.0	35.0	39.0	32.0	39.0
12	36.309910924654275	38.0	35.0	39.0	32.0	39.0
13	36.35794321129517	38.0	35.0	39.0	32.0	39.0
14	37.28378483298988	39.0	36.0	41.0	32.0	41.0
15	37.33843063928291	39.0	36.0	41.0	32.0	41.0
16	37.316794863103325	39.0	36.0	41.0	32.0	41.0
17	37.41815494845568	39.0	36.0	41.0	32.0	41.0
18	37.43588631654376	39.0	36.0	41.0	32.0	41.0
19	37.53464333692251	39.0	36.0	41.0	32.0	41.0
20	37.49656730985184	39.0	36.0	41.0	32.0	41.0
21	37.370796815118	39.0	36.0	41.0	31.0	41.0
22	37.325609228174	39.0	36.0	41.0	31.0	41.0
23	37.199870926154084	39.0	36.0	41.0	31.0	41.0
24	37.13044260436783	39.0	36.0	41.0	31.0	41.0
25	37.04234099355805	39.0	36.0	40.0	31.0	41.0
26	36.7659832090379	39.0	35.0	40.0	30.0	41.0
27	36.60981105149357	38.0	35.0	40.0	30.0	41.0
28	36.46507412467064	38.0	35.0	40.0	30.0	41.0
29	36.326013341418125	38.0	35.0	40.0	30.0	41.0
30	36.14747671614624	38.0	35.0	40.0	30.0	41.0
31	35.882109902895415	38.0	35.0	40.0	29.0	41.0
32	35.58571082837913	38.0	34.0	40.0	28.0	41.0
33	35.73765930185744	38.0	34.0	40.0	29.0	41.0
34	35.70457125175498	38.0	34.0	40.0	29.0	41.0
35	35.611503252032215	38.0	34.0	40.0	28.0	41.0
36	35.52238889631636	38.0	34.0	40.0	27.0	41.0
37	35.37871357157709	38.0	34.0	40.0	27.0	41.0
38	35.25022108819705	38.0	34.0	40.0	26.0	41.0
39	35.18240884064947	38.0	34.0	40.0	25.0	41.0
40	35.009834684732965	38.0	34.0	40.0	25.0	41.0
41	34.8631074907143	38.0	33.0	40.0	24.0	41.0
42	34.62933422816453	38.0	33.0	40.0	23.0	41.0
43	34.324219639146186	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	3.0
15	6.0
16	21.0
17	77.0
18	176.0
19	440.0
20	882.0
21	1778.0
22	3260.0
23	5762.0
24	9703.0
25	15308.0
26	23440.0
27	34232.0
28	47692.0
29	64519.0
30	83579.0
31	101294.0
32	124763.0
33	152487.0
34	184497.0
35	222292.0
36	279551.0
37	369109.0
38	456996.0
39	458480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.188115661982565	22.409423905703374	13.918160061416124	22.484300370897937
2	17.518479564633314	22.91931862037935	36.68848322702794	22.873718587959395
3	19.891802182211517	25.984443723159323	32.313569153168764	21.81018494146039
4	12.775280843555151	15.465531261208271	35.248484196596735	36.51070369863984
5	11.275024627426147	40.54168596651427	34.539827878814506	13.643461527245075
6	31.84147247201033	35.66687585618416	15.462577106284057	17.02907456552145
7	24.87830207294566	35.377406547392034	21.028848837786217	18.715442541876094
8	28.06174486781861	33.27419973647423	20.040381025387173	18.623674370319986
9	25.374486478870782	13.645658206547695	23.723416866482424	37.2564384480991
10	16.454756549228907	27.59525350625997	33.58904447858976	22.36094546592136
11	31.434708063214373	21.524957496149185	25.440235362825142	21.600099077811304
12	20.554934215135955	27.176558856423906	31.76602790009957	20.502479028340574
13	31.40111401939668	20.623675127795607	28.91504115554421	19.060169697263508
14	21.173488807729584	22.42923189320806	28.489832215362437	27.90744708369992
15	23.089447644989356	28.80456333613473	27.09732690640518	21.008662112470738
16	21.940697991061032	28.27789053644045	27.120089048834075	22.661322423664448
17	19.41368356986141	27.022033829618735	27.80234734120376	25.7619352593161
18	20.65961734604024	26.1932418782517	32.50986896050484	20.637271815203217
19	19.778786819469698	24.55868523441409	33.972251395554146	21.690276550562064
20	24.5416799067093	23.340361444642355	32.54641715924675	19.57154148940159
21	23.979254257675784	24.364582106380634	31.24480892488076	20.411354711062817
22	22.064469507629482	24.092534736885163	32.15162086527198	21.69137489021338
23	21.007033539884308	26.06636471163471	33.29677250999773	19.629829238483246
24	19.474092250683526	25.295557519100694	31.595671632803086	23.63467859741269
25	20.94393582060553	26.20865650715114	32.836757565003715	20.01065010723961
26	22.433435882907904	25.05763442635803	31.345098697179807	21.16383099355426
27	20.85523542531688	26.715597066902895	30.937122327389293	21.492045180390924
28	20.61557013864455	26.042049744181544	31.127551698658017	22.214828418515886
29	19.658954176133534	26.106094307987316	32.09651451380102	22.138437002078135
30	18.915984212693097	26.364658611418413	34.240094775349775	20.479262400538715
31	20.64208178540034	25.721183070874343	32.77422795244114	20.862507191284184
32	21.79378559425288	25.894417745532884	30.622050342587286	21.689746317626952
33	22.78604078475989	25.79802897268505	30.144196846704734	21.27173339585032
34	20.84413840746053	28.844709544079212	30.1136705791545	20.19748146930576
35	19.343692822426124	29.899418599586646	30.488090778908393	20.268797799078833
36	20.416013186135622	26.00027496365064	32.74692095628267	20.836790893931067
37	20.540011945390553	26.393556306382226	31.706187325993646	21.360244422233578
38	20.76592904953095	25.924072916118284	32.43949947525876	20.870498559092
39	21.932895992158613	25.314759526108098	32.15355242810704	20.598792053626244
40	19.93312247736947	26.278988118616137	31.345136570960886	22.442752833053508
41	21.013434208886782	25.654373721049755	31.2203045885222	22.111887481541263
42	18.345832312319317	27.524202292954453	31.70194546251272	22.428019932213505
43	19.123040173855806	27.248632661818572	31.23996108090256	22.388366083423062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	237.0
1	313.0
2	389.0
3	1162.5
4	1936.0
5	1936.0
6	3411.5
7	4887.0
8	5234.5
9	5582.0
10	8548.5
11	11515.0
12	11515.0
13	21034.5
14	30554.0
15	50401.5
16	70249.0
17	69187.5
18	68126.0
19	68126.0
20	71894.5
21	75663.0
22	61111.5
23	46560.0
24	50609.0
25	54658.0
26	54658.0
27	58677.0
28	62696.0
29	65548.0
30	68400.0
31	72819.0
32	77238.0
33	77238.0
34	84042.5
35	90847.0
36	98524.0
37	106201.0
38	110921.0
39	115641.0
40	115641.0
41	122638.5
42	129636.0
43	148640.0
44	167644.0
45	216911.0
46	266178.0
47	266178.0
48	270607.0
49	275036.0
50	251294.5
51	227553.0
52	212869.0
53	198185.0
54	198185.0
55	172303.5
56	146422.0
57	119933.0
58	93444.0
59	82635.5
60	71827.0
61	71827.0
62	63013.0
63	54199.0
64	47541.0
65	40883.0
66	34529.0
67	28175.0
68	28175.0
69	23670.0
70	19165.0
71	15993.0
72	12821.0
73	10321.0
74	7821.0
75	7821.0
76	6114.0
77	4407.0
78	3444.0
79	2481.0
80	1997.0
81	1513.0
82	1513.0
83	1114.5
84	716.0
85	550.0
86	384.0
87	324.0
88	264.0
89	264.0
90	218.0
91	172.0
92	99.5
93	27.0
94	20.5
95	14.0
96	14.0
97	8.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2640349.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.733816891190685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.75720026579368	27.794302557197526
2	14.007283706616056	10.851111244700503
3	5.575583345045958	6.478908730457479
4	2.7046889854523832	4.190517116405317
5	1.5509848142319855	3.003778089773957
6	0.9147842472228918	2.1259851316126324
7	0.6039707784858588	1.6375865479050802
8	0.4733887602378244	1.4668922845919727
9	0.32577545182262424	1.1356674028688194
>10	1.8023603808290107	12.988310190971411
>50	0.1665168917693607	4.515505040702425
>100	0.10061848137893172	7.395273995126889
>500	0.007584676115441928	2.026724308302522
>1k	0.007584676115441885	5.795088511661374
>5k	9.850228721353096E-4	2.833331586198286
>10k+	6.895160104947167E-4	5.7610172615236825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34454	1.304903253319921	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33786	1.2796035675586825	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30105	1.1401901794043137	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19626	0.7433108274701564	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11759	0.4453577917161709	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11058	0.4188082711793024	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10207	0.38657768348047933	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9738	0.36881488015410085	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9549	0.36165673553003785	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8232	0.31177696584807535	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7785	0.29484738570545027	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7759	0.29386266739737815	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7443	0.2818945525761935	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6325	0.23955166532909095	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6088	0.2305755792132025	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5862	0.2220161046891907	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5480	0.20754832031674603	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4227	0.1600924726238842	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4221	0.15986522993740598	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4159	0.15751705551046472	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3765	0.14259478576506363	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3715	0.14070109671107872	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3632	0.13755757288146378	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3549	0.1344140490518488	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3364	0.12740739955210467	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2894	0.10960672244464652	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	2768	0.10483462602860455	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2767	0.10479675224752485	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2653	0.10047914120443927	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.7873781079698175E-5	0.0
3	0.0	0.0	0.0	3.7873781079698175E-5	0.0
4	0.0	0.0	0.0	7.574756215939635E-5	0.0
5	0.0	0.0	0.0	7.574756215939635E-5	0.0
6	3.7873781079698175E-5	0.0	0.0	7.574756215939635E-5	0.0
7	3.7873781079698175E-5	0.0	0.0	7.574756215939635E-5	0.0
8	7.574756215939635E-5	0.0	0.0	7.574756215939635E-5	0.0
9	7.574756215939635E-5	0.0	0.0	1.893689053984909E-4	0.0
10	7.574756215939635E-5	0.0	0.0	1.893689053984909E-4	0.0
11	7.574756215939635E-5	0.0	0.0	3.029902486375854E-4	0.0
12	7.574756215939635E-5	0.0	0.0	5.302329351157745E-4	0.0
13	7.574756215939635E-5	0.0	0.0	6.817280594345671E-4	0.0
14	7.574756215939635E-5	0.0	0.0	8.332231837533599E-4	0.0
15	7.574756215939635E-5	0.0	0.0	0.0013255823377894362	0.0
16	7.574756215939635E-5	0.0	0.0	0.0023481744269412868	0.0
17	7.574756215939635E-5	0.0	0.0	0.0037873781079698176	0.0
18	7.574756215939635E-5	0.0	0.0	0.004620601291723177	0.0
19	7.574756215939635E-5	0.0	0.0	0.005453824475476537	0.0
20	7.574756215939635E-5	0.0	0.0	0.0069309019375847665	0.0
21	7.574756215939635E-5	0.0	0.0	0.00954419283208394	0.0
22	7.574756215939635E-5	0.0	0.0	0.016323599645349914	0.0
23	7.574756215939635E-5	0.0	0.0	0.02677676322334661	0.0
24	7.574756215939635E-5	0.0	0.0	0.0415854116255086	0.0
25	7.574756215939635E-5	0.0	0.0	0.04828907087661517	0.0
26	7.574756215939635E-5	0.0	0.0	0.05590170087363451	0.0
27	7.574756215939635E-5	0.0	0.0	0.11646187682007189	0.0
28	7.574756215939635E-5	0.0	0.0	0.18520278947972407	0.0
29	7.574756215939635E-5	0.0	0.0	0.2656845742740827	0.0
30	7.574756215939635E-5	0.0	0.0	0.437555792813753	0.0
31	7.574756215939635E-5	0.0	0.0	0.6766908465509673	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7855	0.0	28.756842	1
ATTGGAC	925	0.0	25.4	3
GTATTGG	1100	0.0	23.377274	1
TCGCTAA	45	0.0038271025	20.555557	14
TTGGACC	1615	0.0	20.504644	4
TATTGGA	1235	0.0	19.923077	2
GGACCCT	1650	0.0	19.060604	6
TGGACCC	1730	0.0	19.034681	5
TTGGACG	60	9.2411425E-4	18.5	4
TGCGACG	60	9.2411425E-4	18.5	22
CGTCTGT	830	0.0	17.831326	34
ATCACGT	95	3.6099718E-6	17.526316	23
TAATACG	65	0.0015806678	17.076923	4
GACCCTC	1840	0.0	16.790762	7
GTATCAA	13620	0.0	16.639133	2
GCCGATA	125	1.6599915E-7	16.279999	2
ATGCGCT	125	1.6599915E-7	16.279999	35
CGACGAG	115	1.2436121E-6	16.086956	24
ACGCACC	70	0.0025936887	15.857142	24
TATACCG	140	3.4784534E-8	15.857142	5
>>END_MODULE