FastQCFastQC Report
Wed 25 May 2016
SRR2088160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3364310
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT393471.1695414512931328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT384861.143949279347028No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT348821.0368247872520666No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG238730.709595726909827No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131950.3922052367350214No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA121720.3617978129244927No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA116570.3464900677999352No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT115450.34316100478255573No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT113940.33867271446448155No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA96070.2855563250711141No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT90450.26885156243033487No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT87740.2607964188793542No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG86950.258448240501024No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA69420.20634245952364677No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA68700.2042023475839028No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA66850.19870344884983845No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT60440.17965050783072906No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA50300.14951059801266828No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC46840.1392261711911209No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45320.13470815709610587No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC44050.13093323742461307No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42260.12561268135219406No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG41490.12332395052774565No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38970.1158335587386418No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38410.11416902722995205No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82300.031.335361
GTATTGG10750.022.21
ATTGGAC11350.021.5154193
TATTGGA12000.020.81252
TAAAGCG450.003827318520.5555554
TTGGACC19350.018.6434124
GGACCCT20250.018.1802466
GTATCAA150800.017.1137282
TGGACCC21000.017.0023825
GACCCTC20900.016.7296667
CGTCTGT10750.016.17674434
ACGGACC1802.0190782E-1015.4166668
GACCGTC1853.0559022E-1015.022
ACCCTCG23700.014.9092848
TAATGCG750.00410659214.85
GTACGGG1803.3378456E-914.38888837
ACGTTTA1551.2122655E-714.32258126
ACCGACC3000.014.1833338
TGCGTCT11950.014.08786632
CTAGCGG1356.5755085E-613.70370429
Produced by FastQC (version 0.11.5)