##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3364310
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.073562483837698	31.0	31.0	33.0	28.0	34.0
2	31.217396434930194	31.0	31.0	34.0	28.0	34.0
3	31.27625991659508	31.0	31.0	34.0	28.0	34.0
4	35.247396642996634	37.0	35.0	37.0	33.0	37.0
5	35.101380372201135	37.0	35.0	37.0	32.0	37.0
6	35.14646331640069	37.0	35.0	37.0	32.0	37.0
7	34.98978245167657	37.0	35.0	37.0	32.0	37.0
8	35.047224245090376	37.0	35.0	37.0	32.0	37.0
9	36.552226756749526	39.0	35.0	39.0	32.0	39.0
10	36.39853313160797	38.0	35.0	39.0	32.0	39.0
11	36.41389348781771	38.0	35.0	39.0	32.0	39.0
12	36.251762768591476	38.0	35.0	39.0	32.0	39.0
13	36.31183244112463	38.0	35.0	39.0	32.0	39.0
14	37.25217117328665	39.0	36.0	41.0	32.0	41.0
15	37.30489491158662	39.0	36.0	41.0	32.0	41.0
16	37.286545235129935	39.0	36.0	41.0	32.0	41.0
17	37.37125205465608	39.0	36.0	41.0	32.0	41.0
18	37.36861377221481	39.0	36.0	41.0	32.0	41.0
19	37.46083327636276	39.0	36.0	41.0	32.0	41.0
20	37.41611563738181	39.0	36.0	41.0	32.0	41.0
21	37.29639569480815	39.0	36.0	41.0	31.0	41.0
22	37.25756663327696	39.0	36.0	41.0	31.0	41.0
23	37.16148898288208	39.0	36.0	40.0	31.0	41.0
24	37.09231075614316	39.0	36.0	40.0	31.0	41.0
25	36.99191988847639	39.0	36.0	40.0	31.0	41.0
26	36.73772839007107	39.0	35.0	40.0	30.0	41.0
27	36.57507869369945	38.0	35.0	40.0	30.0	41.0
28	36.43906477108233	38.0	35.0	40.0	30.0	41.0
29	36.308194250827064	38.0	35.0	40.0	30.0	41.0
30	36.14931977136471	38.0	35.0	40.0	30.0	41.0
31	35.91080102606478	38.0	35.0	40.0	29.0	41.0
32	35.6428078863125	38.0	34.0	40.0	28.0	41.0
33	35.80135391803966	38.0	34.0	40.0	29.0	41.0
34	35.79163454021776	38.0	34.0	40.0	29.0	41.0
35	35.699159114350344	38.0	34.0	40.0	28.0	41.0
36	35.62394458299027	38.0	34.0	40.0	27.0	41.0
37	35.49612193882252	38.0	34.0	40.0	27.0	41.0
38	35.37580900689889	38.0	34.0	40.0	27.0	41.0
39	35.33350226346561	38.0	34.0	40.0	26.0	41.0
40	35.186399885860695	38.0	34.0	40.0	25.0	41.0
41	35.06564228623403	38.0	34.0	40.0	25.0	41.0
42	34.867873055693444	38.0	33.0	40.0	24.0	41.0
43	34.56987049350387	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	1.0
13	0.0
14	7.0
15	10.0
16	27.0
17	95.0
18	231.0
19	531.0
20	1105.0
21	2302.0
22	4269.0
23	7523.0
24	12373.0
25	19704.0
26	29637.0
27	43715.0
28	60690.0
29	82922.0
30	105590.0
31	131327.0
32	158556.0
33	193214.0
34	234337.0
35	283169.0
36	353373.0
37	459449.0
38	580725.0
39	599427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.04936822112112	22.428878432724687	13.71883090440536	22.802922441748827
2	17.484773995262028	22.98964720849149	36.5951116276443	22.93046716860218
3	19.669204086424855	26.234621660905205	32.15182310785867	21.944351144811268
4	12.763270923309683	15.706757106211972	35.81578392003115	35.71418805044719
5	11.239095089334812	40.44689698630626	34.969072410093005	13.344935514265927
6	31.586566041773796	36.34879068813516	15.60831195698375	16.456331313107295
7	24.925586524428486	35.02180239038614	21.537135400721098	18.515475684464274
8	27.708534588073036	33.57880219123683	20.28148416763021	18.431179053059914
9	25.427621116960093	13.545035980631987	23.46876476900167	37.55857813340626
10	16.202371362924346	27.502340747434097	34.08045037466821	22.214837514973354
11	31.75287057375807	21.699902803249405	25.29930357190627	21.247923051086257
12	20.289569034958134	27.177400417916303	31.915846042724954	20.617184504400605
13	31.093181068331994	20.689859139021078	28.684752594142633	19.5322071985043
14	21.0145319545464	22.17658301405043	28.533250503074925	28.275634528328247
15	23.053345262475812	28.847193035124587	26.949300153671928	21.150161548727674
16	21.869476950697173	28.393786541668277	27.030951368928548	22.705785138706005
17	19.556432076711122	27.05574099889725	27.908962015985445	25.47886490840618
18	20.663345529989805	26.455350428468243	32.11226076075035	20.769043280791603
19	20.001456465070103	24.830470438217645	33.39445532664945	21.773617770062806
20	24.359556640143147	23.784520451444724	32.18796722061879	19.667955687793338
21	23.667408770297623	24.776283992854403	30.91174713388481	20.644560102963165
22	22.03795131839813	24.286406425091624	31.96277988651462	21.71286236999563
23	21.09778825375783	26.07069503107621	32.935668829566836	19.895847885599128
24	19.73664733630373	25.35253885640741	31.374516617077497	23.536297190211368
25	20.994498128888242	26.274837931106234	32.53261441424839	20.19804952575714
26	22.404861620956453	25.272879134205827	31.096093998472195	21.226165246365525
27	20.853964111511722	26.78311451679542	30.7529032699127	21.610018101780156
28	20.742024367552336	26.055833142605767	30.925628137716206	22.276514352125695
29	19.838956576534265	26.120363462344432	31.847719145976438	22.192960815144858
30	19.239041586536317	26.30735574307956	33.709200400676515	20.744402269707606
31	20.79341677788313	25.82122337121133	32.419455995434426	20.965903855471108
32	21.8176684074892	25.87228881999578	30.55229749933865	21.757745273176372
33	22.661912843941252	25.872437438880485	30.09143033787017	21.37421937930809
34	20.9910501707631	28.50557766674296	30.09469995333367	20.408672209160272
35	19.65131037270644	29.383201904699625	30.429300510357248	20.536187212236683
36	20.738398066765548	25.957536612262246	32.244680187022006	21.0593851339502
37	20.78063555379855	26.11825307418163	31.514307540030494	21.586803831989325
38	20.98266806566577	25.75559327172585	32.09442649458582	21.16731216802257
39	21.790976455796283	25.336606911967092	32.01354809752966	20.858868534706968
40	20.11211808662103	26.095960241475964	31.16439923788236	22.627522434020648
41	21.17744797595941	25.545089483430484	31.025083895360417	22.252378645249696
42	18.657376995580073	27.368197342099865	31.42944615686426	22.5449795054558
43	19.40270070237285	27.029970484289496	31.13140584547797	22.435922967859682
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	292.0
2	410.0
3	1303.0
4	2196.0
5	2196.0
6	3728.5
7	5261.0
8	5795.5
9	6330.0
10	9562.5
11	12795.0
12	12795.0
13	23549.0
14	34303.0
15	56534.5
16	78766.0
17	78297.5
18	77829.0
19	77829.0
20	82857.0
21	87885.0
22	72286.5
23	56688.0
24	61995.5
25	67303.0
26	67303.0
27	73889.5
28	80476.0
29	85164.0
30	89852.0
31	97283.0
32	104714.0
33	104714.0
34	115847.0
35	126980.0
36	138110.0
37	149240.0
38	155536.5
39	161833.0
40	161833.0
41	171609.0
42	181385.0
43	204914.5
44	228444.0
45	282851.0
46	337258.0
47	337258.0
48	339812.5
49	342367.0
50	311830.0
51	281293.0
52	262012.5
53	242732.0
54	242732.0
55	212427.0
56	182122.0
57	149987.0
58	117852.0
59	104226.5
60	90601.0
61	90601.0
62	79941.0
63	69281.0
64	60351.5
65	51422.0
66	43240.0
67	35058.0
68	35058.0
69	29458.0
70	23858.0
71	19863.5
72	15869.0
73	12786.5
74	9704.0
75	9704.0
76	7556.0
77	5408.0
78	4227.5
79	3047.0
80	2382.5
81	1718.0
82	1718.0
83	1253.5
84	789.0
85	653.5
86	518.0
87	413.0
88	308.0
89	308.0
90	250.0
91	192.0
92	112.5
93	33.0
94	22.0
95	11.0
96	11.0
97	6.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3364310.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.676064958518225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.99793451828045	28.565947268264363
2	14.225088023704693	11.28791032938294
3	5.525597715059881	6.5770192163206715
4	2.675233042765742	4.245708799357917
5	1.4882317256196895	2.9523589309507234
6	0.9016820403495551	2.146511712290292
7	0.5767633667585932	1.6018590563645327
8	0.40558390109810577	1.2873578564878139
9	0.28915229030984696	1.0325182547914027
>10	1.61716849478696	12.323772431944565
>50	0.1733803100500976	4.827635722791559
>100	0.10840613940728656	7.846919743814031
>500	0.008153448016329548	2.177672175190942
>1k	0.006266075790226226	5.211831904681239
>5k	6.794540013498318E-4	2.0276056350744405
>10k+	6.794540013498318E-4	5.8873709622926835
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39347	1.1695414512931328	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38486	1.143949279347028	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34882	1.0368247872520666	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23873	0.709595726909827	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13195	0.3922052367350214	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	12172	0.3617978129244927	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11657	0.3464900677999352	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11545	0.34316100478255573	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	11394	0.33867271446448155	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	9607	0.2855563250711141	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	9045	0.26885156243033487	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	8774	0.2607964188793542	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8695	0.258448240501024	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6942	0.20634245952364677	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6870	0.2042023475839028	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6685	0.19870344884983845	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6044	0.17965050783072906	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5030	0.14951059801266828	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4684	0.1392261711911209	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4532	0.13470815709610587	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4405	0.13093323742461307	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4226	0.12561268135219406	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4149	0.12332395052774565	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3897	0.1158335587386418	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3841	0.11416902722995205	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.9723776940888324E-5	0.0	0.0	0.0	0.0
3	2.9723776940888324E-5	0.0	0.0	2.9723776940888324E-5	0.0
4	2.9723776940888324E-5	0.0	0.0	2.9723776940888324E-5	0.0
5	2.9723776940888324E-5	0.0	0.0	5.944755388177665E-5	0.0
6	2.9723776940888324E-5	0.0	0.0	5.944755388177665E-5	0.0
7	2.9723776940888324E-5	0.0	0.0	5.944755388177665E-5	0.0
8	1.188951077635533E-4	0.0	0.0	5.944755388177665E-5	0.0
9	1.188951077635533E-4	0.0	0.0	5.944755388177665E-5	0.0
10	1.188951077635533E-4	0.0	0.0	5.944755388177665E-5	0.0
11	1.188951077635533E-4	0.0	0.0	1.188951077635533E-4	0.0
12	1.188951077635533E-4	2.9723776940888324E-5	0.0	2.9723776940888323E-4	0.0
13	1.188951077635533E-4	2.9723776940888324E-5	0.0	3.566853232906599E-4	0.0
14	1.188951077635533E-4	2.9723776940888324E-5	0.0	5.647517618768782E-4	0.0
15	1.188951077635533E-4	2.9723776940888324E-5	0.0	8.917133082266497E-4	0.0
16	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.0016645315086897463	0.0
17	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.002645416147739061	0.0
18	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.003388510571261269	0.0
19	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.0041613287717243656	0.0
20	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.00567724139570967	0.0
21	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.008768514197562055	0.0
22	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.014951059801266828	0.0
23	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.025235486622814187	0.0
24	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.03959207088526325	0.0
25	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.04562599760426358	0.0
26	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.053473074716658096	0.0
27	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.11467433143794716	0.0
28	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.17881824207638417	0.0
29	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.2556542054685805	0.0
30	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.4075724294134607	0.0
31	1.188951077635533E-4	2.9723776940888324E-5	0.0	0.6244965535280637	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8230	0.0	31.33536	1
GTATTGG	1075	0.0	22.2	1
ATTGGAC	1135	0.0	21.515419	3
TATTGGA	1200	0.0	20.8125	2
TAAAGCG	45	0.0038273185	20.555555	4
TTGGACC	1935	0.0	18.643412	4
GGACCCT	2025	0.0	18.180246	6
GTATCAA	15080	0.0	17.113728	2
TGGACCC	2100	0.0	17.002382	5
GACCCTC	2090	0.0	16.729666	7
CGTCTGT	1075	0.0	16.176744	34
ACGGACC	180	2.0190782E-10	15.416666	8
GACCGTC	185	3.0559022E-10	15.0	22
ACCCTCG	2370	0.0	14.909284	8
TAATGCG	75	0.004106592	14.8	5
GTACGGG	180	3.3378456E-9	14.388888	37
ACGTTTA	155	1.2122655E-7	14.322581	26
ACCGACC	300	0.0	14.183333	8
TGCGTCT	1195	0.0	14.087866	32
CTAGCGG	135	6.5755085E-6	13.703704	29
>>END_MODULE