FastQCFastQC Report
Wed 25 May 2016
SRR2088159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3833605
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT450761.1758123228658144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT444181.1586483218797972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT397961.0380829532515738No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG260980.6807691454909934No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152220.39706751217196345No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA144450.3767993833480497No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA132270.3450277219484011No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT128850.3361066150529332No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT127400.33232427441011786No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA107600.28067576080477774No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT100350.2617640575907012No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG97770.2550340997572781No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT94970.2477302695504623No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA79170.2065157990977161No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA77730.2027595435627823No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA77470.2020813307578637No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT65700.17137915878135593No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.13921622076348503No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC53310.1390597101161961No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA53250.1389031994689072No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC50110.13071247559412094No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG49360.12875609250300957No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG47590.12413902840798674No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT43800.11425277252090395No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41700.10877489986579213No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC39650.10342745275008772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102550.029.351051
GTATTGG14250.023.4982451
ATTGGAC15250.022.8065573
TATTGGA17650.019.6005672
TTGGACC24150.019.3809534
GGACCCT24150.018.9213266
TGGACCC24950.018.4629275
TAGGTCG1850.018.021
GTATCAA177350.017.0448272
GACCCTC26700.016.6292137
TATACCG2250.016.4444455
ACGGACC1708.54925E-1116.3235288
CGGGATA1708.54925E-1116.32352824
CTGTGCG2950.016.3050849
CATTGCG2055.456968E-1215.34146429
GTCGTCA2300.015.28260924
TAGTACT6400.014.74218754
ACCCTCG29700.014.7003368
GTATCGT908.2802284E-414.3888896
AGGTCGT2700.014.38888922
Produced by FastQC (version 0.11.5)