##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088159_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3833605
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.11239394773327	31.0	31.0	33.0	30.0	34.0
2	31.256108806201997	31.0	31.0	34.0	28.0	34.0
3	31.31061755188654	31.0	31.0	34.0	28.0	34.0
4	35.26707055108703	37.0	35.0	37.0	33.0	37.0
5	35.139308562045386	37.0	35.0	37.0	32.0	37.0
6	35.1863105353838	37.0	35.0	37.0	32.0	37.0
7	35.022748822583445	37.0	35.0	37.0	32.0	37.0
8	35.0824855455896	37.0	35.0	37.0	32.0	37.0
9	36.58422998717917	39.0	35.0	39.0	32.0	39.0
10	36.43369230789296	38.0	35.0	39.0	32.0	39.0
11	36.45774173395537	38.0	35.0	39.0	32.0	39.0
12	36.296208399143886	38.0	35.0	39.0	32.0	39.0
13	36.34224965795902	38.0	35.0	39.0	32.0	39.0
14	37.275657246899456	39.0	36.0	41.0	32.0	41.0
15	37.33143529393352	39.0	36.0	41.0	32.0	41.0
16	37.31676894202715	39.0	36.0	41.0	32.0	41.0
17	37.40807021067637	39.0	36.0	41.0	32.0	41.0
18	37.414384111039084	39.0	36.0	41.0	32.0	41.0
19	37.50778888278787	39.0	36.0	41.0	32.0	41.0
20	37.4685114924464	39.0	36.0	41.0	32.0	41.0
21	37.34896735579174	39.0	36.0	41.0	31.0	41.0
22	37.30932946925935	39.0	36.0	41.0	31.0	41.0
23	37.193986078377925	39.0	36.0	41.0	31.0	41.0
24	37.125746392755644	39.0	36.0	41.0	31.0	41.0
25	37.03528871649531	39.0	36.0	40.0	31.0	41.0
26	36.7689344624707	39.0	35.0	40.0	30.0	41.0
27	36.62367875667942	38.0	35.0	40.0	30.0	41.0
28	36.48401621972008	38.0	35.0	40.0	30.0	41.0
29	36.35436801652752	38.0	35.0	40.0	30.0	41.0
30	36.18393757311982	38.0	35.0	40.0	30.0	41.0
31	35.94033318508297	38.0	35.0	40.0	29.0	41.0
32	35.653574898822384	38.0	34.0	40.0	28.0	41.0
33	35.82238910894576	38.0	34.0	40.0	29.0	41.0
34	35.80465853941656	38.0	34.0	40.0	29.0	41.0
35	35.728633231644885	38.0	34.0	40.0	28.0	41.0
36	35.65269296132492	38.0	34.0	40.0	27.0	41.0
37	35.524572041198816	38.0	34.0	40.0	27.0	41.0
38	35.40532397051861	38.0	34.0	40.0	27.0	41.0
39	35.36427670560739	38.0	34.0	40.0	26.0	41.0
40	35.218787538100564	38.0	34.0	40.0	25.0	41.0
41	35.0904310694503	38.0	34.0	40.0	25.0	41.0
42	34.8772192231594	38.0	34.0	40.0	24.0	41.0
43	34.57893210176844	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	5.0
15	27.0
16	38.0
17	103.0
18	250.0
19	602.0
20	1314.0
21	2626.0
22	4776.0
23	8262.0
24	13651.0
25	21736.0
26	33607.0
27	48332.0
28	68783.0
29	92106.0
30	118796.0
31	145959.0
32	178536.0
33	217717.0
34	265638.0
35	322678.0
36	402704.0
37	528921.0
38	671240.0
39	685193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.945141713869845	22.595989936365378	13.815560027702384	22.643308322062396
2	17.341510145150583	23.04462770681904	36.89814156648899	22.715720581541397
3	19.628548063767653	26.30448363876821	32.286033641963634	21.7809346555005
4	12.739288476512318	15.718886009382812	35.89749074304734	35.64433477105753
5	11.23793400728557	40.48573079386113	34.9429062201244	13.333428978728898
6	31.484594787412888	36.40351575084026	15.703939242566722	16.40795021918012
7	24.86189891759845	35.10066895259162	21.5897568998371	18.44767522997283
8	27.5526038806815	33.760390024533045	20.319464316224543	18.367541778560913
9	25.342986562256677	13.716332277321216	23.66083621030336	37.27984495011875
10	16.15659411963413	27.593818351134246	34.056116892585436	22.193470636646182
11	31.63067139154921	21.81215852963464	25.39823482074966	21.158935258066496
12	20.33537623203225	27.140354835722512	32.0479809474372	20.476287984808035
13	31.04326084716605	20.75698983072069	28.716286628382427	19.483462693730836
14	20.97002690678878	22.34215575157065	28.594234408604958	28.093582933035616
15	22.91540208237416	28.94380093932474	26.987313507781842	21.153483470519262
16	21.790533975200887	28.56441913029642	27.12770355839999	22.5173433361027
17	19.48335835329931	27.1162782811479	28.080383868447583	25.319979497105205
18	20.610834971260733	26.528815566548978	32.24153244791782	20.618817014272466
19	19.897798547320342	24.910860665091995	33.604609760264815	21.58673102732285
20	24.219292284938067	23.846640433743175	32.33207385737445	19.601993423944304
21	23.62588216574217	24.82115397908757	31.10727370190721	20.44569015326305
22	21.984581092731254	24.39573195464843	32.05087639441205	21.568810558208266
23	21.00678082379379	26.18457039783702	32.9599424040818	19.848706374287385
24	19.76111258202136	25.44821388745058	31.439676231640973	23.35099729888708
25	20.997155418985525	26.423275220060493	32.45900399232576	20.120565368628224
26	22.318366133182735	25.4094514171387	31.129472128714358	21.14271032096421
27	20.85337430434278	26.838341456670676	30.81125989766812	21.49702434131842
28	20.753703107127624	26.190543887541885	30.999385695709393	22.0563673096211
29	19.831464117977728	26.15498988549942	31.882105746418844	22.131440250104014
30	19.20036101789308	26.481705861715017	33.787335940974614	20.530597179417285
31	20.790196173053825	25.888608763813696	32.51339144225866	20.807803620873823
32	21.676489883543034	25.97427225809649	30.664635506266297	21.684602352094178
33	22.529316400620303	26.00233983417697	30.156106328116746	21.31223743708598
34	20.86148677289392	28.59068683393307	30.191895096130146	20.35593129704286
35	19.574812741531797	29.52338595134345	30.428174003320635	20.473627303804122
36	20.607626502991312	26.004974430073	32.388626371261516	20.998772695674177
37	20.739356297792806	26.223385038364672	31.51297538478795	21.524283279054572
38	20.879355071792737	25.84650739969298	32.221577340388485	21.0525601881258
39	21.70870499177667	25.44823997255847	32.08843894976139	20.75461608590348
40	20.02892838464057	26.261756232058335	31.230969283481215	22.47834609981988
41	21.018623462771984	25.719055562584042	31.14796647020233	22.114354504441643
42	18.56124978968882	27.46094081158596	31.547851174025492	22.429958224699728
43	19.25847863825303	27.185377731925954	31.177051365490183	22.379092264330833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	242.0
1	378.0
2	514.0
3	1581.5
4	2649.0
5	2649.0
6	4539.0
7	6429.0
8	6949.0
9	7469.0
10	11122.5
11	14776.0
12	14776.0
13	27237.0
14	39698.0
15	65538.0
16	91378.0
17	90800.0
18	90222.0
19	90222.0
20	96685.0
21	103148.0
22	85339.5
23	67531.0
24	73679.5
25	79828.0
26	79828.0
27	87340.0
28	94852.0
29	100017.0
30	105182.0
31	113829.5
32	122477.0
33	122477.0
34	134582.5
35	146688.0
36	159531.0
37	172374.0
38	179324.0
39	186274.0
40	186274.0
41	196080.5
42	205887.0
43	230855.5
44	255824.0
45	317423.0
46	379022.0
47	379022.0
48	382451.5
49	385881.0
50	351505.0
51	317129.0
52	295419.0
53	273709.0
54	273709.0
55	238512.0
56	203315.0
57	168923.5
58	134532.0
59	118684.0
60	102836.0
61	102836.0
62	90109.5
63	77383.0
64	67167.0
65	56951.0
66	48365.0
67	39779.0
68	39779.0
69	33387.0
70	26995.0
71	22516.5
72	18038.0
73	14343.5
74	10649.0
75	10649.0
76	8387.5
77	6126.0
78	4755.0
79	3384.0
80	2683.0
81	1982.0
82	1982.0
83	1514.0
84	1046.0
85	851.0
86	656.0
87	525.0
88	394.0
89	394.0
90	331.5
91	269.0
92	162.5
93	56.0
94	40.5
95	25.0
96	25.0
97	15.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3833605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.88791031313994
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.99934028625387	27.61015977343944
2	14.459706726670282	11.246155566821205
3	5.739984497857607	6.6964800705450065
4	2.825801961287118	4.395581329329136
5	1.6044242665239787	3.119635349040491
6	1.0323857408594697	2.4088394459464557
7	0.6336574140638123	1.7249128881158797
8	0.43249151931870855	1.3454953131567655
9	0.32019510145824936	1.120654654939363
>10	1.6575221093516346	12.011135007713845
>50	0.164984969899494	4.47792549088818
>100	0.11292136972588579	8.094560212626064
>500	0.009033580984510785	2.4095047745025595
>1k	0.0061347452931167865	5.081197769026403
>5k	6.74147834408438E-4	1.8426056537079478
>10k+	7.415626178492819E-4	6.415156700201212
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45076	1.1758123228658144	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44418	1.1586483218797972	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	39796	1.0380829532515738	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	26098	0.6807691454909934	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15222	0.39706751217196345	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	14445	0.3767993833480497	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	13227	0.3450277219484011	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	12885	0.3361066150529332	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	12740	0.33232427441011786	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	10760	0.28067576080477774	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	10035	0.2617640575907012	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9777	0.2550340997572781	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	9497	0.2477302695504623	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	7917	0.2065157990977161	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7773	0.2027595435627823	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	7747	0.2020813307578637	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	6570	0.17137915878135593	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5337	0.13921622076348503	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	5331	0.1390597101161961	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5325	0.1389031994689072	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	5011	0.13071247559412094	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4936	0.12875609250300957	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4759	0.12413902840798674	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4380	0.11425277252090395	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4170	0.10877489986579213	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3965	0.10342745275008772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.608510788148492E-5	0.0	0.0	0.0
3	0.0	2.608510788148492E-5	0.0	0.0	0.0
4	0.0	2.608510788148492E-5	0.0	0.0	0.0
5	0.0	2.608510788148492E-5	0.0	0.0	0.0
6	2.608510788148492E-5	2.608510788148492E-5	0.0	0.0	0.0
7	2.608510788148492E-5	2.608510788148492E-5	0.0	2.608510788148492E-5	0.0
8	5.217021576296984E-5	2.608510788148492E-5	0.0	5.217021576296984E-5	0.0
9	5.217021576296984E-5	2.608510788148492E-5	0.0	1.304255394074246E-4	0.0
10	5.217021576296984E-5	5.217021576296984E-5	0.0	1.8259575517039445E-4	0.0
11	5.217021576296984E-5	5.217021576296984E-5	0.0	2.869361866963341E-4	0.0
12	5.217021576296984E-5	5.217021576296984E-5	0.0	5.217021576296984E-4	0.0
13	5.217021576296984E-5	5.217021576296984E-5	0.0	5.738723733926682E-4	0.0
14	5.217021576296984E-5	5.217021576296984E-5	0.0	8.086383443260325E-4	0.0
15	5.217021576296984E-5	5.217021576296984E-5	0.0	0.0011477447467853365	0.0
16	5.217021576296984E-5	5.217021576296984E-5	0.0	0.0019563830911113692	0.0
17	5.217021576296984E-5	5.217021576296984E-5	0.0	0.003417149132474525	0.0
18	5.217021576296984E-5	5.217021576296984E-5	0.0	0.0040431917216301625	0.0
19	5.217021576296984E-5	5.217021576296984E-5	0.0	0.004825744958074711	0.0
20	5.217021576296984E-5	5.217021576296984E-5	0.0	0.005947404596978562	0.0
21	5.217021576296984E-5	5.217021576296984E-5	0.0	0.00868634092453448	0.0
22	5.217021576296984E-5	5.217021576296984E-5	0.0	0.015651064728890954	0.0
23	5.217021576296984E-5	5.217021576296984E-5	0.0	0.02655463982335165	0.0
24	5.217021576296984E-5	5.217021576296984E-5	0.0	0.04142315131579805	0.0
25	5.217021576296984E-5	5.217021576296984E-5	0.0	0.04710970483396177	0.0
26	5.217021576296984E-5	5.217021576296984E-5	0.0	0.05451787547230348	0.0
27	5.217021576296984E-5	5.217021576296984E-5	0.0	0.11464404913912622	0.0
28	5.217021576296984E-5	5.217021576296984E-5	0.0	0.18165669128666098	0.0
29	5.217021576296984E-5	5.217021576296984E-5	0.0	0.26737235578522045	0.0
30	5.217021576296984E-5	5.217021576296984E-5	0.0	0.42393517328989294	0.0
31	5.217021576296984E-5	5.217021576296984E-5	0.0	0.6689786767285623	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10255	0.0	29.35105	1
GTATTGG	1425	0.0	23.498245	1
ATTGGAC	1525	0.0	22.806557	3
TATTGGA	1765	0.0	19.600567	2
TTGGACC	2415	0.0	19.380953	4
GGACCCT	2415	0.0	18.921326	6
TGGACCC	2495	0.0	18.462927	5
TAGGTCG	185	0.0	18.0	21
GTATCAA	17735	0.0	17.044827	2
GACCCTC	2670	0.0	16.629213	7
TATACCG	225	0.0	16.444445	5
ACGGACC	170	8.54925E-11	16.323528	8
CGGGATA	170	8.54925E-11	16.323528	24
CTGTGCG	295	0.0	16.305084	9
CATTGCG	205	5.456968E-12	15.341464	29
GTCGTCA	230	0.0	15.282609	24
TAGTACT	640	0.0	14.7421875	4
ACCCTCG	2970	0.0	14.700336	8
GTATCGT	90	8.2802284E-4	14.388889	6
AGGTCGT	270	0.0	14.388889	22
>>END_MODULE