FastQCFastQC Report
Wed 25 May 2016
SRR2088158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876418
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT254531.3564674821921343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT238391.270452532431473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT218951.1668508829056212No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100750.5369272731342377No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG92470.49280064463248596No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT71780.3825373664076981No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA55670.2966822957358115No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT50980.27168786485740387No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA49020.26124243105747225No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG43320.23086540419032434No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT42670.22740135726687763No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA40460.21562359772715886No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA39870.2124793089812611No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT39610.21109369021188243No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38520.20528474998641028No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA36150.19265430197322772No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33550.17879811427944095No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT31640.1686191456274668No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA29710.1583335909163097No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28430.15151208312859926No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25870.13786906755317846No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24940.13291281580117012No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC23630.12593142892468523No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG23500.12523861953999588No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC23490.1251853265104044No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC21870.1165518557165834No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA21420.11415366938496646No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20950.11164889699416655No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20800.11084950155029424No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG20560.10957046884009854No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA19250.10258908196361366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55500.029.2666661
ATAGTCG502.7029848E-422.230
GTATTGG3900.020.3974341
GCGCATA555.14476E-420.18181826
ATCCCGT500.007036499718.519
TGCCGTC1002.877332E-718.535
ATCCACG2350.017.3191491
ATTGGAC4500.017.2666653
GGACCCT9000.017.2666656
CACGGCT2600.017.0769234
TAGTCGC650.001580437317.07692331
GTATCAA96050.017.0265482
GCCGTCA1005.881777E-616.65000213
GACCCTC9350.016.620327
TTGGACC9900.016.2575764
TGGACCC10200.016.1421575
ACGGCTC2200.015.9772735
TCCGACG700.002593313315.85714223
CGCATAG700.002593313315.85714227
TTAGCAC1302.591314E-715.6538463
Produced by FastQC (version 0.11.5)