##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1876418
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.984806157263467	31.0	31.0	33.0	28.0	34.0
2	31.13885605446121	31.0	31.0	34.0	28.0	34.0
3	31.194828657580562	31.0	31.0	34.0	28.0	34.0
4	35.15499158503063	35.0	35.0	37.0	32.0	37.0
5	35.00801100820819	35.0	35.0	37.0	32.0	37.0
6	35.06607482980871	36.0	35.0	37.0	32.0	37.0
7	34.88871349560706	36.0	35.0	37.0	32.0	37.0
8	34.96975620570683	36.0	35.0	37.0	32.0	37.0
9	36.417631892254285	38.0	35.0	39.0	32.0	39.0
10	36.270382185632414	38.0	35.0	39.0	32.0	39.0
11	36.313490917268965	38.0	35.0	39.0	32.0	39.0
12	36.16361599600942	38.0	35.0	39.0	31.0	39.0
13	36.2093302238627	38.0	35.0	39.0	32.0	39.0
14	37.12182253634318	39.0	36.0	41.0	31.0	41.0
15	37.18014056569485	39.0	36.0	41.0	31.0	41.0
16	37.15377543809535	39.0	36.0	41.0	31.0	41.0
17	37.25960153867635	39.0	36.0	41.0	32.0	41.0
18	37.27412122458855	39.0	36.0	40.0	32.0	41.0
19	37.3798108950138	39.0	36.0	41.0	32.0	41.0
20	37.33443507789842	39.0	36.0	41.0	31.0	41.0
21	37.2234065117687	39.0	36.0	41.0	31.0	41.0
22	37.18283932471336	39.0	36.0	40.0	31.0	41.0
23	37.06223133651457	39.0	36.0	40.0	31.0	41.0
24	36.976572384191584	39.0	35.0	40.0	31.0	41.0
25	36.882876843006194	39.0	35.0	40.0	31.0	41.0
26	36.580824741608744	38.0	35.0	40.0	30.0	41.0
27	36.39782873538839	38.0	35.0	40.0	30.0	41.0
28	36.22087882337517	38.0	35.0	40.0	30.0	41.0
29	36.07007766926132	38.0	35.0	40.0	30.0	41.0
30	35.8792886233238	38.0	34.0	40.0	29.0	41.0
31	35.589609564606604	38.0	34.0	40.0	28.0	41.0
32	35.27200762303495	37.0	34.0	40.0	27.0	41.0
33	35.38651462520611	38.0	34.0	40.0	27.0	41.0
34	35.32470696827679	38.0	34.0	40.0	27.0	41.0
35	35.221211371879825	38.0	34.0	40.0	26.0	41.0
36	35.09525809281301	38.0	34.0	40.0	25.0	41.0
37	34.91838918620478	38.0	33.0	40.0	25.0	41.0
38	34.75115885692847	38.0	33.0	40.0	24.0	41.0
39	34.62667966306015	38.0	33.0	40.0	24.0	41.0
40	34.417614838484816	38.0	33.0	40.0	23.0	41.0
41	34.23177511620545	38.0	33.0	40.0	21.0	41.0
42	33.994535865675985	38.0	33.0	40.0	19.0	41.0
43	33.64377819867428	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	5.0
15	5.0
16	29.0
17	63.0
18	201.0
19	431.0
20	944.0
21	1752.0
22	3030.0
23	5232.0
24	8391.0
25	13179.0
26	19626.0
27	28210.0
28	38824.0
29	50909.0
30	64367.0
31	78230.0
32	94665.0
33	114319.0
34	136369.0
35	161918.0
36	199712.0
37	257355.0
38	308975.0
39	289677.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.03516274092446	23.681663680480575	14.399936474708728	21.883237103886234
2	17.496901010329253	23.938109738874815	35.65532839697765	22.909660853818288
3	21.02079600600719	26.485942897584653	31.544304094290293	20.948957002117865
4	13.932450019132197	16.264606287085286	34.65336614762809	35.149577546154426
5	11.177680026518612	41.209261475854525	33.99615650670586	13.616901990920999
6	31.752786426052193	35.46203457864932	15.556182044725642	17.228996950572846
7	23.6871528625285	35.79287770635327	22.09401103592057	18.425958395197657
8	27.352007921475916	33.64666081864488	20.69437619975933	18.30695506011987
9	25.226735194396984	14.195824171373332	24.994697343555647	35.58274329067404
10	16.502826129359235	28.58856608708721	33.64202432507042	21.266583458483133
11	30.53514728594588	22.53261266945851	25.265585812969178	21.666654231626428
12	19.857195997906647	28.092034930383313	31.914690649951133	20.136078421758903
13	29.95505265884254	22.03363003339341	29.702870042815622	18.308447264948427
14	20.626534173089365	23.482720801015553	29.53451736233611	26.356227663558972
15	22.709758699820615	29.435712085473494	27.741260209612147	20.11326900509375
16	21.21073236347125	28.794223888280758	28.49183923837866	21.503204509869338
17	18.598308052896527	27.909772769180428	29.182463608854743	24.3094555690683
18	19.391787970484188	27.256506812447974	33.66003736907235	19.69166784799549
19	18.72770352874466	25.916720048517973	35.32789602316754	20.02768039956982
20	23.053765205833667	24.804707693061996	33.78921967280212	18.352307428302222
21	22.21940953454934	25.81050704054214	32.37967233313686	19.59041109177166
22	20.5361491949022	25.586463144139525	33.32775532956943	20.54963233138885
23	19.74762552906655	27.75186552250085	34.045345973018804	18.455162975413796
24	18.60832714245973	26.812362703832516	32.54706573908373	22.03224441462403
25	19.522515772072108	27.858131823506277	33.64586142320101	18.973490981220603
26	20.963399413137157	26.781559332728634	32.25390078330095	20.00114047083326
27	19.748158459362468	28.000370919485956	31.944961090759094	20.306509530392482
28	19.36343607874152	27.787571852327147	32.149179980153676	20.699812088777662
29	18.451272584253616	27.45704848280074	33.15902959788277	20.93264933506287
30	18.04992277840012	27.41153623552961	35.236658356506915	19.301882629563348
31	19.4474258933777	27.055965142095207	33.73065063328107	19.765958331246022
32	20.061574766390006	27.577224264529544	32.08522834464389	20.27597262443656
33	21.10883609089233	27.413188319446945	31.38250645645054	20.09546913321019
34	19.483878325618278	29.53387784598101	31.487227259597812	19.4950165688029
35	18.187578673834935	30.153356021952465	31.576013446897228	20.083051857315372
36	19.16470637139486	26.957266451291765	33.54412502971086	20.333902147602505
37	19.049753306566018	26.890703457332	33.12385619835239	20.935687037749585
38	19.3710569819731	26.741642853564613	33.53144128866809	20.3558588757942
39	20.514192466710508	25.853301343304103	33.378277121622155	20.254229068363234
40	18.74161300946804	26.523727655565015	32.85867008310515	21.875989251861792
41	19.24853630694227	26.16725058062756	32.476718940022955	22.107494172407215
42	16.983209498096908	27.70496765646034	32.90498172582016	22.4068411196226
43	17.539855192179992	27.384783134674684	32.576696663536595	22.498665009608736
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	323.0
1	396.0
2	469.0
3	1192.5
4	1916.0
5	1916.0
6	3260.5
7	4605.0
8	5207.0
9	5809.0
10	8752.0
11	11695.0
12	11695.0
13	20715.0
14	29735.0
15	44187.0
16	58639.0
17	57804.5
18	56970.0
19	56970.0
20	59730.5
21	62491.0
22	51862.5
23	41234.0
24	44159.5
25	47085.0
26	47085.0
27	49695.5
28	52306.0
29	54222.5
30	56139.0
31	59658.0
32	63177.0
33	63177.0
34	67474.0
35	71771.0
36	76887.5
37	82004.0
38	85038.5
39	88073.0
40	88073.0
41	92198.5
42	96324.0
43	107988.0
44	119652.0
45	151756.5
46	183861.0
47	183861.0
48	184886.5
49	185912.0
50	167528.0
51	149144.0
52	135082.0
53	121020.0
54	121020.0
55	106163.0
56	91306.0
57	74416.0
58	57526.0
59	49729.0
60	41932.0
61	41932.0
62	36532.0
63	31132.0
64	26063.0
65	20994.0
66	17871.5
67	14749.0
68	14749.0
69	12555.5
70	10362.0
71	8789.5
72	7217.0
73	5874.5
74	4532.0
75	4532.0
76	3632.5
77	2733.0
78	2167.0
79	1601.0
80	1285.5
81	970.0
82	970.0
83	720.5
84	471.0
85	368.5
86	266.0
87	232.0
88	198.0
89	198.0
90	128.5
91	59.0
92	34.5
93	10.0
94	7.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1876418.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.9543091064066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.68327811078089	29.76693438615837
2	13.54456955818926	11.094169767986159
3	5.452837251225582	6.69951546880863
4	2.640744777972504	4.325995116328599
5	1.5167563836332487	3.1058854887215745
6	0.9262578755578341	2.276055080870342
7	0.6188019756031296	1.7739825169153949
8	0.41946320370742274	1.3743060562717913
9	0.31416410687384194	1.157973654874453
>10	1.6386056731307017	12.472880538654014
>50	0.1429599485687022	4.069144467864909
>100	0.08373917217072617	6.822976143174321
>500	0.008255762080356544	2.355761264465215
>1k	0.008517849765447227	6.889374040765454
>5k	5.24175370181368E-4	1.453868838235851
>10k+	5.24175370181368E-4	4.361177169904825
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25453	1.3564674821921343	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23839	1.270452532431473	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21895	1.1668508829056212	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10075	0.5369272731342377	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9247	0.49280064463248596	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7178	0.3825373664076981	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5567	0.2966822957358115	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5098	0.27168786485740387	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4902	0.26124243105747225	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4332	0.23086540419032434	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4267	0.22740135726687763	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4046	0.21562359772715886	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3987	0.2124793089812611	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	3961	0.21109369021188243	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3852	0.20528474998641028	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3615	0.19265430197322772	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3355	0.17879811427944095	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3164	0.1686191456274668	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2971	0.1583335909163097	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2843	0.15151208312859926	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2587	0.13786906755317846	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2494	0.13291281580117012	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2363	0.12593142892468523	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2350	0.12523861953999588	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC	2349	0.1251853265104044	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2187	0.1165518557165834	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2142	0.11415366938496646	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2095	0.11164889699416655	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2080	0.11084950155029424	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2056	0.10957046884009854	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1925	0.10258908196361366	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0658605918297522E-4	0.0
3	0.0	0.0	0.0	2.1317211836595045E-4	0.0
4	0.0	0.0	0.0	4.796372663233885E-4	0.0
5	0.0	0.0	0.0	5.329302959148761E-4	0.0
6	5.329302959148761E-5	0.0	0.0	5.329302959148761E-4	0.0
7	5.329302959148761E-5	0.0	0.0	6.395163550978513E-4	0.0
8	5.329302959148761E-5	0.0	0.0	6.395163550978513E-4	0.0
9	5.329302959148761E-5	0.0	0.0	0.0011191536214212399	0.0
10	5.329302959148761E-5	0.0	0.0	0.0017053769469276036	0.0
11	5.329302959148761E-5	0.0	0.0	0.001971842094885042	0.0
12	5.329302959148761E-5	0.0	0.0	0.0022916002724339673	0.0
13	1.0658605918297522E-4	0.0	0.0	0.0026113584499828928	0.0
14	1.0658605918297522E-4	0.0	0.0	0.002877823597940331	0.0
15	1.0658605918297522E-4	0.0	0.0	0.0031975817754892567	0.0
16	1.0658605918297522E-4	0.0	0.0	0.004210149337727521	0.0
17	1.0658605918297522E-4	0.0	0.0	0.005862233255063637	0.0
18	1.0658605918297522E-4	0.0	0.0	0.006768214758118927	0.0
19	1.0658605918297522E-4	0.0	0.0	0.007514317172399753	0.0
20	1.0658605918297522E-4	0.0	0.0	0.00889993594177843	0.0
21	1.0658605918297522E-4	0.0	0.0	0.012097517717267688	0.0
22	1.0658605918297522E-4	0.0	0.0	0.017959750972331327	0.0
23	1.0658605918297522E-4	0.0	0.0	0.027978840535530996	0.0
24	1.0658605918297522E-4	0.0	0.0	0.04364699123542835	0.0
25	1.0658605918297522E-4	0.0	0.0	0.05569121592310455	0.0
26	1.0658605918297522E-4	0.0	0.0	0.07295815751074654	0.0
27	1.0658605918297522E-4	0.0	0.0	0.19910275855379772	0.0
28	1.0658605918297522E-4	0.0	0.0	0.3145354606489599	0.0
29	1.0658605918297522E-4	0.0	0.0	0.4326328142236964	0.0
30	1.0658605918297522E-4	0.0	0.0	0.6614197902599528	0.0
31	1.0658605918297522E-4	0.0	0.0	0.9907174201057547	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5550	0.0	29.266666	1
ATAGTCG	50	2.7029848E-4	22.2	30
GTATTGG	390	0.0	20.397434	1
GCGCATA	55	5.14476E-4	20.181818	26
ATCCCGT	50	0.0070364997	18.5	19
TGCCGTC	100	2.877332E-7	18.5	35
ATCCACG	235	0.0	17.319149	1
ATTGGAC	450	0.0	17.266665	3
GGACCCT	900	0.0	17.266665	6
CACGGCT	260	0.0	17.076923	4
TAGTCGC	65	0.0015804373	17.076923	31
GTATCAA	9605	0.0	17.026548	2
GCCGTCA	100	5.881777E-6	16.650002	13
GACCCTC	935	0.0	16.62032	7
TTGGACC	990	0.0	16.257576	4
TGGACCC	1020	0.0	16.142157	5
ACGGCTC	220	0.0	15.977273	5
TCCGACG	70	0.0025933133	15.857142	23
CGCATAG	70	0.0025933133	15.857142	27
TTAGCAC	130	2.591314E-7	15.653846	3
>>END_MODULE