FastQCFastQC Report
Wed 25 May 2016
SRR2088157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences898130
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116711.299477803881398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115851.289902352666095No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT92681.031921882132876No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39800.4431429748477392No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36390.4051751973545032No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT26420.29416676873058467No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22610.25174529299767295No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT22500.2505205259817621No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21640.2409450747664592No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA19960.22223954215982097No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT18640.2075423379688909No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG16420.1828243127386904No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT15590.17358288889136317No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15160.16879516328371172No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA14960.16656831416387383No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT13570.15109171278100053No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA12510.13928941244585974No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12490.13906672753387594No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA11420.12715308474274326No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA11030.1228107289590594No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC9650.10744547003217796No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9380.10443922372039682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22400.032.7879451
TGCGTTA551.9020592E-523.54545437
ACGTTTA603.7251186E-521.58333426
AACTGCG603.7251186E-521.5833347
CGGGATA555.1418925E-420.18181824
GTATCAA39550.018.6169412
CTTAACG701.2188207E-418.530
ACTGCGT500.007033713618.58
ATCACGT701.2188207E-418.523
AATGCGT801.6160291E-518.535
GGACCCT3100.017.9032276
ACCGACC852.722043E-517.4117648
CTAGGAC852.722043E-517.4117643
TATACCG650.001579570817.0769215
GCCGTCA650.001579570817.07692113
GACCCTC3150.017.0317467
GTATTGG1851.8189894E-1217.01
TCCACGG1107.802937E-716.8181822
CGACCGC904.4442153E-516.44444510
TAACGCT904.4442153E-516.44444532
Produced by FastQC (version 0.11.5)