##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088157_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	898130
Sequences flagged as poor quality	0
Sequence length	43
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81386770289379	31.0	31.0	33.0	28.0	34.0
2	30.992828432409564	31.0	31.0	34.0	28.0	34.0
3	31.03472437175019	31.0	31.0	34.0	27.0	34.0
4	35.001170209212475	35.0	35.0	37.0	32.0	37.0
5	34.82853261777248	35.0	35.0	37.0	32.0	37.0
6	34.90814692750493	35.0	35.0	37.0	32.0	37.0
7	34.72512776546825	35.0	35.0	37.0	31.0	37.0
8	34.795420484785055	35.0	35.0	37.0	32.0	37.0
9	36.24305501430751	38.0	35.0	39.0	32.0	39.0
10	36.050374667364416	38.0	35.0	39.0	31.0	39.0
11	36.12316034427087	38.0	35.0	39.0	31.0	39.0
12	35.951482524801534	37.0	35.0	39.0	30.0	39.0
13	35.99109037667153	38.0	35.0	39.0	30.0	39.0
14	36.86264460601472	38.0	36.0	40.0	31.0	41.0
15	36.915015643615064	38.0	36.0	40.0	31.0	41.0
16	36.887803547370645	38.0	36.0	40.0	31.0	41.0
17	36.9842962600069	38.0	36.0	40.0	31.0	41.0
18	36.991153841871444	38.0	36.0	40.0	31.0	41.0
19	37.10776056918264	39.0	36.0	40.0	31.0	41.0
20	37.075602641043055	39.0	36.0	40.0	31.0	41.0
21	36.971470722501195	39.0	36.0	40.0	31.0	41.0
22	36.93117254740405	39.0	36.0	40.0	31.0	41.0
23	36.808582276507856	39.0	35.0	40.0	31.0	41.0
24	36.732039905136226	39.0	35.0	40.0	30.0	41.0
25	36.63612283299745	38.0	35.0	40.0	30.0	41.0
26	36.34570162448643	38.0	35.0	40.0	30.0	41.0
27	36.172058610668834	38.0	35.0	40.0	30.0	41.0
28	36.03304644093839	38.0	34.0	40.0	29.0	41.0
29	35.87669157026266	38.0	34.0	40.0	29.0	41.0
30	35.6895159943438	38.0	34.0	40.0	28.0	41.0
31	35.42654738178215	38.0	34.0	40.0	27.0	41.0
32	35.11305044926681	37.0	33.0	40.0	27.0	41.0
33	35.273699798470155	38.0	34.0	40.0	27.0	41.0
34	35.24875018093149	38.0	34.0	40.0	27.0	41.0
35	35.17291706100453	38.0	34.0	40.0	26.0	41.0
36	35.056096556177835	38.0	33.0	40.0	25.0	41.0
37	34.911488314609244	38.0	33.0	40.0	25.0	41.0
38	34.79204012782114	38.0	33.0	40.0	25.0	41.0
39	34.71885918519591	38.0	33.0	40.0	24.0	41.0
40	34.550588444879914	38.0	33.0	40.0	24.0	41.0
41	34.41553115918631	38.0	33.0	40.0	23.0	41.0
42	34.189925734581855	38.0	33.0	40.0	22.0	41.0
43	33.85505661763887	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	9.0
16	13.0
17	39.0
18	94.0
19	193.0
20	442.0
21	867.0
22	1570.0
23	2679.0
24	4371.0
25	6680.0
26	9991.0
27	14116.0
28	19528.0
29	25386.0
30	32272.0
31	38714.0
32	46908.0
33	56453.0
34	66842.0
35	79478.0
36	98874.0
37	125333.0
38	151326.0
39	115952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.830792869629114	24.52829768519034	15.06263013149544	21.5782793136851
2	17.67249729994544	24.8186788104172	35.493748121096054	22.015075768541305
3	20.078385089018294	26.724638972086446	32.87007448810306	20.3269014507922
4	13.89052809726877	17.482101700199305	35.998352131651316	32.629018070880605
5	10.890962332847138	41.337556923830626	35.02521906628216	12.74626167704007
6	30.619732110050883	37.850645229532475	15.684032378386203	15.845590282030441
7	23.310767928918978	36.18718893701357	22.394308173649694	18.10773496041776
8	26.65538396445949	34.97210871477403	20.7500027835614	17.62250453720508
9	24.688296794450693	14.311736608286104	24.69520002672219	36.30476657054101
10	15.700845089240978	29.162148018660993	34.876799572444966	20.260207319653055
11	30.860343157449364	23.15121418948259	25.13199648157839	20.85644617148965
12	19.101243695233432	28.929887655461904	32.461559017068794	19.507309632235867
13	29.669869617984034	22.3118034137597	29.39875073764377	18.619576230612495
14	20.2143342277844	23.667620500372998	29.68746172603075	26.430583545811853
15	22.081435872312472	30.580428223085743	27.44135036130627	19.896785543295515
16	20.688653090309867	29.307895293554388	28.43430238384198	21.569149232293768
17	18.570919577344036	28.790486900560055	29.530802890450158	23.107790631645752
18	19.143887855878326	27.68185006624876	33.49526237849754	19.678999699375368
19	19.077416409651164	26.660951087259082	34.795296894658904	19.466335608430853
20	22.116286061037936	25.852382171845946	33.7279681115206	18.303363655595515
21	21.739614532417356	26.48747954082371	32.55742487167782	19.215481055081113
22	20.288488303474995	26.49438277309521	33.13584893055571	20.081279992874084
23	19.75671673365771	28.01320521528064	33.71282553750571	18.517252513555942
24	18.653647022146014	27.456270250409183	32.63959560419984	21.250487123244966
25	19.551178559896673	28.155946243862246	33.41253493369557	18.880340262545513
26	20.46719294534199	27.35929097124024	32.3694788059635	19.804037277454267
27	19.598944473517196	28.436083863137853	32.040239163595466	19.924732499749478
28	19.34230011245588	28.281429191765113	32.22885328404574	20.14741741173327
29	18.603208889581687	27.930143743110687	32.99221716232616	20.474430204981463
30	18.231547771480745	28.220413525881554	34.44846514424415	19.09957355839355
31	19.456092102479598	27.751327758787703	33.417879371583176	19.374700767149523
32	20.050438132564327	28.185117967332125	31.926335831115765	19.838108068987786
33	20.709140102212377	28.036141761214967	31.615133666618416	19.639584469954237
34	19.424693529889883	29.962588934786723	31.550443699687126	19.06227383563627
35	18.28599423246078	30.45449990535891	31.83158340106666	19.42792246111365
36	19.243873381359048	27.737855321612688	33.322458886798124	19.695812410230147
37	19.021077126919266	27.7733735650741	32.83689443621747	20.368654871789165
38	19.388395889236527	27.654571164530743	33.120706356540815	19.836326589691915
39	20.144299822965493	27.11990469085767	33.06369901907296	19.672096467103874
40	18.696179840334917	27.70523198200706	32.575016979724545	21.02357119793348
41	19.254673599590262	27.03005132887221	32.48527496019507	21.230000111342456
42	17.31486533129948	28.34422633694454	32.784674824357275	21.556233507398705
43	17.79029761838487	28.163851558237674	32.52424481979224	21.521606003585227
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	196.0
2	230.0
3	478.5
4	727.0
5	727.0
6	1348.5
7	1970.0
8	2131.0
9	2292.0
10	3373.0
11	4454.0
12	4454.0
13	8116.5
14	11779.0
15	18707.5
16	25636.0
17	25159.0
18	24682.0
19	24682.0
20	27551.0
21	30420.0
22	26320.0
23	22220.0
24	24121.5
25	26023.0
26	26023.0
27	28039.5
28	30056.0
29	31420.5
30	32785.0
31	34996.0
32	37207.0
33	37207.0
34	40169.0
35	43131.0
36	45119.0
37	47107.0
38	47685.5
39	48264.0
40	48264.0
41	49207.0
42	50150.0
43	54101.0
44	58052.0
45	68629.5
46	79207.0
47	79207.0
48	79625.5
49	80044.0
50	72307.0
51	64570.0
52	59071.0
53	53572.0
54	53572.0
55	46533.5
56	39495.0
57	32818.0
58	26141.0
59	22687.0
60	19233.0
61	19233.0
62	16378.5
63	13524.0
64	11346.5
65	9169.0
66	7522.5
67	5876.0
68	5876.0
69	4881.5
70	3887.0
71	3213.5
72	2540.0
73	2038.0
74	1536.0
75	1536.0
76	1225.5
77	915.0
78	699.0
79	483.0
80	389.5
81	296.0
82	296.0
83	222.5
84	149.0
85	107.5
86	66.0
87	56.0
88	46.0
89	46.0
90	37.5
91	29.0
92	16.0
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	898130.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.171077166817604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.26848531205435	39.53911624466246
2	12.768412276570999	13.067468198043116
3	4.392998307296363	6.743833661290839
4	1.9616478835197477	4.015185408868537
5	0.9731343423843173	2.48981662639141
6	0.592606367766937	1.8194583704729657
7	0.37812768156713195	1.354444054066708
8	0.2605863668185246	1.0667588068073088
9	0.18516388874732437	0.8527532075637629
>10	1.0536232329034823	9.929477102625729
>50	0.0946278359809357	3.3285806709138317
>100	0.058130062451034935	5.731430135722662
>500	0.008085760556793675	2.721962438133292
>1k	0.0037150791747430404	3.7026785378181435
>5k	2.1853406910253177E-4	1.0364055657787006
>10k+	4.3706813820506353E-4	2.6006309708404682
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11671	1.299477803881398	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11585	1.289902352666095	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9268	1.031921882132876	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3980	0.4431429748477392	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3639	0.4051751973545032	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2642	0.29416676873058467	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2261	0.25174529299767295	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2250	0.2505205259817621	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2164	0.2409450747664592	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1996	0.22223954215982097	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1864	0.2075423379688909	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1642	0.1828243127386904	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1559	0.17358288889136317	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1516	0.16879516328371172	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1496	0.16656831416387383	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1357	0.15109171278100053	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1251	0.13928941244585974	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1249	0.13906672753387594	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1142	0.12715308474274326	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1103	0.1228107289590594	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	965	0.10744547003217796	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	938	0.10443922372039682	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.1134245599189427E-4	0.0
10	0.0	0.0	0.0	1.1134245599189427E-4	0.0
11	0.0	0.0	0.0	1.1134245599189427E-4	0.0
12	0.0	0.0	0.0	4.453698239675771E-4	0.0
13	0.0	0.0	0.0	4.453698239675771E-4	0.0
14	0.0	0.0	0.0	7.793971919432599E-4	0.0
15	0.0	0.0	0.0	0.0010020821039270485	0.0
16	0.0	0.0	0.0	0.0015587943838865199	0.0
17	0.0	0.0	0.0	0.0018928217518622025	0.0
18	0.0	0.0	0.0	0.0023381915758297796	0.0
19	0.0	0.0	0.0	0.0027835613997973566	0.0
20	0.0	0.0	0.0	0.003340273679756828	0.0
21	0.0	0.0	0.0	0.0038969859597162994	0.0
22	0.0	0.0	0.0	0.006012492623562291	0.0
23	0.0	0.0	0.0	0.010911560687205639	0.0
24	0.0	0.0	0.0	0.01926224488659771	0.0
25	0.0	0.0	0.0	0.02560876487813568	0.0
26	0.0	0.0	0.0	0.03384810662153586	0.0
27	0.0	0.0	0.0	0.10766815494416176	0.0
28	0.0	0.0	0.0	0.1808201485308363	0.0
29	0.0	0.0	0.0	0.2647723603487246	0.0
30	0.0	0.0	0.0	0.42065179873737657	0.0
31	0.0	0.0	0.0	0.6480130938728247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2240	0.0	32.787945	1
TGCGTTA	55	1.9020592E-5	23.545454	37
ACGTTTA	60	3.7251186E-5	21.583334	26
AACTGCG	60	3.7251186E-5	21.583334	7
CGGGATA	55	5.1418925E-4	20.181818	24
GTATCAA	3955	0.0	18.616941	2
CTTAACG	70	1.2188207E-4	18.5	30
ACTGCGT	50	0.0070337136	18.5	8
ATCACGT	70	1.2188207E-4	18.5	23
AATGCGT	80	1.6160291E-5	18.5	35
GGACCCT	310	0.0	17.903227	6
ACCGACC	85	2.722043E-5	17.411764	8
CTAGGAC	85	2.722043E-5	17.411764	3
TATACCG	65	0.0015795708	17.076921	5
GCCGTCA	65	0.0015795708	17.076921	13
GACCCTC	315	0.0	17.031746	7
GTATTGG	185	1.8189894E-12	17.0	1
TCCACGG	110	7.802937E-7	16.818182	2
CGACCGC	90	4.4442153E-5	16.444445	10
TAACGCT	90	4.4442153E-5	16.444445	32
>>END_MODULE