FastQCFastQC Report
Wed 25 May 2016
SRR2088156_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088156_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences562347
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112692.003922844791561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT106011.885134978936493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59861.0644673128868831No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.6298602108662444No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT18810.33449098154698076No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT17450.31030662562439204No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14090.2505570404038788No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA12640.2247722491628834No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG12560.2233496399909664No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12250.2178370294497881No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG11540.2052113730490249No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT11420.20307745929114943No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA10320.1835165831772909No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA10240.18209397400537392No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA9030.16057701028012952No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.14777352773287666No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7410.1317691745488106No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7260.12910178235146627No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC6880.12234438878486059No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA6710.11932134429453702No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT6530.1161204736577238No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT6310.11220829843495209No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.10687351404026339No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5900.10491742642887755No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC5670.10082742505961621No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA5660.10064959891312658No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA5630.10011612047365773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20300.032.352221
CGTCGAG250.005493984629.620
GTATCAA29450.022.3005082
GAGAGCG450.003823414220.5555557
ATACACC450.003823414220.5555554
TAGACTG555.138605E-420.1818185
GGCTTAG555.138605E-420.1818181
CGTCTGT1600.019.6562534
GTATTGG801.6142789E-518.51
GTACACT500.007030521518.54
TAGGTAG609.2288177E-418.55
CGTTGAC701.21779216E-418.534
GCCGCAT500.007030521518.535
TGATACC3350.017.11940437
GTAATAC650.001578577617.0769233
TGCGTCT1757.2759576E-1216.91428632
TAATACT904.4394616E-516.4444454
TATACTG1251.6531703E-716.2799995
GGACCCT1659.695214E-1015.696976
CGTGGAT2950.015.6779663
Produced by FastQC (version 0.11.5)