##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088156_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	562347
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.15298561208649	31.0	30.0	31.0	27.0	34.0
2	30.286828239503368	31.0	30.0	31.0	27.0	34.0
3	30.215511063453704	31.0	30.0	31.0	26.0	34.0
4	34.14071205145577	35.0	33.0	37.0	30.0	37.0
5	33.84368548245123	35.0	33.0	37.0	28.0	37.0
6	34.17208236195801	35.0	35.0	37.0	30.0	37.0
7	33.99214364084809	35.0	33.0	37.0	30.0	37.0
8	34.14327808274962	35.0	33.0	37.0	30.0	37.0
9	35.46349851604081	37.0	35.0	39.0	30.0	39.0
10	35.163815224407706	37.0	34.0	39.0	29.0	39.0
11	35.38650157287226	37.0	34.0	39.0	30.0	39.0
12	35.10003076392334	37.0	34.0	39.0	29.0	39.0
13	35.24536451692638	37.0	34.0	39.0	30.0	39.0
14	35.892171559553084	38.0	34.0	40.0	27.0	41.0
15	36.03573949892149	38.0	34.0	40.0	29.0	41.0
16	35.89716669600798	38.0	34.0	40.0	28.0	41.0
17	36.02623824791454	38.0	34.0	40.0	29.0	41.0
18	36.053756844083814	38.0	34.0	40.0	29.0	41.0
19	36.17375037121208	38.0	34.0	40.0	29.0	41.0
20	36.14847594101151	38.0	34.0	40.0	29.0	41.0
21	36.088054172957264	38.0	34.0	40.0	29.0	41.0
22	36.016472035949334	38.0	34.0	40.0	29.0	41.0
23	35.84641333553838	38.0	34.0	40.0	28.0	41.0
24	35.73557963321579	38.0	34.0	40.0	27.0	41.0
25	35.61132361335616	38.0	34.0	40.0	27.0	41.0
26	35.28515133894197	37.0	34.0	40.0	27.0	41.0
27	35.01628709675698	37.0	33.0	40.0	27.0	41.0
28	34.82484302396919	37.0	33.0	40.0	26.0	41.0
29	34.61946805086539	37.0	33.0	39.0	25.0	40.0
30	34.36415593930438	37.0	33.0	39.0	25.0	40.0
31	34.05253517845743	36.0	33.0	39.0	24.0	40.0
32	33.751580429876924	36.0	32.0	39.0	24.0	40.0
33	33.88817402778	36.0	32.0	39.0	24.0	40.0
34	33.80510432170884	36.0	32.0	39.0	24.0	40.0
35	33.64217822803358	36.0	32.0	39.0	23.0	40.0
36	33.452917860324675	36.0	32.0	39.0	22.0	40.0
37	33.25454034608525	36.0	31.0	39.0	21.0	40.0
38	33.095906975586246	36.0	31.0	39.0	20.0	40.0
39	32.90646344694646	36.0	31.0	39.0	18.0	40.0
40	32.693987875813335	36.0	31.0	39.0	18.0	40.0
41	32.497788731868404	36.0	30.0	39.0	15.0	40.0
42	32.21107963588318	35.0	30.0	39.0	15.0	40.0
43	31.80542440877252	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	4.0
15	15.0
16	38.0
17	80.0
18	172.0
19	380.0
20	691.0
21	1206.0
22	2059.0
23	3209.0
24	5065.0
25	7260.0
26	10010.0
27	13446.0
28	17503.0
29	21910.0
30	26760.0
31	31423.0
32	36874.0
33	42600.0
34	49794.0
35	58234.0
36	69239.0
37	76210.0
38	63817.0
39	24345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.89146736801299	26.559401935104127	15.683910468091767	18.86522022879112
2	22.81296068086075	26.91220900973954	29.852386515798962	20.42244379360075
3	25.334891090376583	25.461147654384213	31.194973921795615	18.008987333443585
4	19.325256469759776	19.62969483255001	31.267882641856364	29.777166055833852
5	11.038913695636325	43.302444931688086	33.26984939903654	12.388791973639051
6	32.0229324598513	37.575020405550305	13.906893786220964	16.495153348377425
7	22.498564053867096	37.30330205371416	22.233958747890537	17.9641751445282
8	27.331523063162066	36.22389734452216	20.123873693644672	16.320705898671108
9	24.43562426757856	13.849989419344283	24.952031396984424	36.76235491609273
10	17.043747010297913	30.455572804691766	33.46261294183129	19.038067243179032
11	31.992702014948065	22.52185927905724	23.805230578272845	21.68020812772185
12	18.047042128792366	30.36808234061887	32.47247695817707	19.112398572411696
13	30.7034624528983	21.578135919636807	30.21764142068865	17.500760206776242
14	19.778535317161825	23.85591103002239	31.232495238704928	25.13305841411086
15	23.221782991640396	30.48260237895819	27.634894469073366	18.660720160328054
16	20.352913770323305	28.215496837361986	30.360080164026837	21.071509228287873
17	18.133287809839832	29.007356667680273	31.43824009019342	21.421115432286474
18	18.55277968940885	26.183121809132082	35.0545126052064	20.20958589625267
19	18.5399762068616	26.09225264827589	37.327130757343774	18.04064038751874
20	21.061017485644985	25.10567318755146	36.59342007692759	17.23988924987597
21	21.22906319407768	24.92624660574343	35.13044436975746	18.71424583042143
22	19.885408831202085	25.36352109996141	35.41923403165661	19.33183603717989
23	19.19473207823639	27.282976525170398	35.79249111313833	17.72980028345488
24	18.15800564420189	26.489694085680192	35.14804915825994	20.20425111185798
25	19.010682016619633	27.21326867574647	35.77488632463586	18.001162982998043
26	19.75719617958307	26.31595794055983	34.866372542220375	19.060473337636726
27	19.855000560152362	26.86988638687501	34.72660119107953	18.548511861893104
28	19.314409074823907	27.58368053888435	34.872774283494	18.22913610279774
29	18.3651731048623	26.59087716303279	35.54015581126955	19.503793920835356
30	17.99138254494111	27.035264703110357	36.77871492156978	18.194637830378753
31	18.990765488212794	26.304577067184496	35.61964409875041	19.085013345852296
32	19.15436554298325	27.571232708630085	34.440834573670706	18.833567174715967
33	20.00668626310801	27.262526518324094	33.656443441504976	19.074343777062914
34	19.547716979018293	28.2169194465339	33.967817024008305	18.267546550439498
35	18.38491180712265	28.52953781206266	34.03432400279543	19.051226378019265
36	19.16325685030773	26.33729707813859	35.261324413573824	19.23812165797986
37	18.797112814685594	26.510499744819484	35.0063217195077	19.68606572098722
38	19.58719438353899	26.465331903611116	34.33218279816555	19.61529091468435
39	20.06661367447501	26.344054471705192	34.22708754559018	19.362244308229617
40	19.223362087821222	26.565092371791792	33.83373610955513	20.377809430831853
41	19.051582030312243	26.75945634990495	33.38668117728022	20.802280442502582
42	17.816935095234793	27.508460078919246	32.96843408073663	21.706170745109336
43	18.387579199319994	26.938171627127023	32.81603707319502	21.858212100357964
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	101.0
1	116.5
2	132.0
3	233.5
4	335.0
5	335.0
6	775.5
7	1216.0
8	1402.5
9	1589.0
10	2415.5
11	3242.0
12	3242.0
13	6441.0
14	9640.0
15	15908.5
16	22177.0
17	20375.0
18	18573.0
19	18573.0
20	21763.0
21	24953.0
22	19795.0
23	14637.0
24	15084.5
25	15532.0
26	15532.0
27	16435.0
28	17338.0
29	17803.0
30	18268.0
31	19221.0
32	20174.0
33	20174.0
34	21804.5
35	23435.0
36	24949.0
37	26463.0
38	27287.5
39	28112.0
40	28112.0
41	29601.0
42	31090.0
43	33468.0
44	35846.0
45	41994.5
46	48143.0
47	48143.0
48	49139.5
49	50136.0
50	44950.5
51	39765.0
52	35527.5
53	31290.0
54	31290.0
55	27590.5
56	23891.0
57	20266.5
58	16642.0
59	14570.5
60	12499.0
61	12499.0
62	10838.5
63	9178.0
64	7846.5
65	6515.0
66	5322.5
67	4130.0
68	4130.0
69	3484.0
70	2838.0
71	2332.0
72	1826.0
73	1500.0
74	1174.0
75	1174.0
76	915.5
77	657.0
78	515.5
79	374.0
80	299.5
81	225.0
82	225.0
83	170.5
84	116.0
85	83.0
86	50.0
87	37.0
88	24.0
89	24.0
90	18.5
91	13.0
92	9.0
93	5.0
94	4.0
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	562347.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.26796739095089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.72748531276531	47.94902898617763
2	9.64196378817939	11.043513356123734
3	2.7768440338168467	4.770726407351391
4	1.1942505735214533	2.7356921160420393
5	0.6508523553872968	1.8636495732321647
6	0.37312714673385716	1.2820939963099849
7	0.2627910566178265	1.0534656762716252
8	0.19192331105549715	0.8792846335271628
9	0.14619606103071597	0.7535116130213259
>10	0.9101042882239156	9.760891123486678
>50	0.06440912350329224	2.554385893531783
>100	0.05102944903108583	5.679165050287469
>500	0.004667327655282229	1.7396908909653441
>1k	0.003422706947206968	2.971177498305454
>5k	3.1115517701881527E-4	1.0666587804279986
>10k+	6.223103540376305E-4	3.897064404938244
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11269	2.003922844791561	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10601	1.885134978936493	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5986	1.0644673128868831	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3542	0.6298602108662444	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1881	0.33449098154698076	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1745	0.31030662562439204	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1409	0.2505570404038788	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1264	0.2247722491628834	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1256	0.2233496399909664	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1225	0.2178370294497881	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1154	0.2052113730490249	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1142	0.20307745929114943	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1032	0.1835165831772909	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1024	0.18209397400537392	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	903	0.16057701028012952	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.14777352773287666	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	741	0.1317691745488106	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	726	0.12910178235146627	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	688	0.12234438878486059	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	671	0.11932134429453702	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	653	0.1161204736577238	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	631	0.11220829843495209	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	601	0.10687351404026339	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	590	0.10491742642887755	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC	567	0.10082742505961621	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	566	0.10064959891312658	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	563	0.10011612047365773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	3.5565229297924593E-4	0.0
15	0.0	0.0	0.0	7.113045859584919E-4	0.0
16	0.0	0.0	0.0	8.891307324481148E-4	0.0
17	0.0	0.0	0.0	0.0012447830254273607	0.0
18	0.0	1.7782614648962296E-4	0.0	0.0016004353184066065	0.0
19	0.0	1.7782614648962296E-4	0.0	0.0019560876113858526	0.0
20	0.0	1.7782614648962296E-4	0.0	0.0030230444903235902	0.0
21	0.0	1.7782614648962296E-4	0.0	0.0048013059552198195	0.0
22	0.0	1.7782614648962296E-4	0.0	0.008002176592033033	0.0
23	0.0	1.7782614648962296E-4	0.0	0.011558699521825493	0.0
24	0.0	1.7782614648962296E-4	0.0	0.020272180699817018	0.0
25	0.0	1.7782614648962296E-4	0.0	0.024362182069078343	0.0
26	0.0	1.7782614648962296E-4	0.0	0.030586097196215148	0.0
27	0.0	1.7782614648962296E-4	0.0	0.08695698563342563	0.0
28	0.0	1.7782614648962296E-4	0.0	0.14688439700042857	0.0
29	0.0	1.7782614648962296E-4	0.0	0.20698963451392113	0.0
30	0.0	1.7782614648962296E-4	0.0	0.34338228887146194	0.0
31	0.0	1.7782614648962296E-4	0.0	0.5528614894362378	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2030	0.0	32.35222	1
CGTCGAG	25	0.0054939846	29.6	20
GTATCAA	2945	0.0	22.300508	2
GAGAGCG	45	0.0038234142	20.555555	7
ATACACC	45	0.0038234142	20.555555	4
TAGACTG	55	5.138605E-4	20.181818	5
GGCTTAG	55	5.138605E-4	20.181818	1
CGTCTGT	160	0.0	19.65625	34
GTATTGG	80	1.6142789E-5	18.5	1
GTACACT	50	0.0070305215	18.5	4
TAGGTAG	60	9.2288177E-4	18.5	5
CGTTGAC	70	1.21779216E-4	18.5	34
GCCGCAT	50	0.0070305215	18.5	35
TGATACC	335	0.0	17.119404	37
GTAATAC	65	0.0015785776	17.076923	3
TGCGTCT	175	7.2759576E-12	16.914286	32
TAATACT	90	4.4394616E-5	16.444445	4
TATACTG	125	1.6531703E-7	16.279999	5
GGACCCT	165	9.695214E-10	15.69697	6
CGTGGAT	295	0.0	15.677966	3
>>END_MODULE