FastQCFastQC Report
Wed 25 May 2016
SRR2088155_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088155_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1780197
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT234611.317887851737757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227101.2757015094396857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT192141.0793187495541223No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG102590.5762845348014854No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77510.43540125053575535No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA63650.3575446987046939No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA61000.34265870574998164No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT58020.3259189853707202No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT58000.32580663825408085No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA51450.2890129575546976No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT44420.24952294605597022No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT43340.24345620175744595No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG42740.24008578825826582No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35010.19666362767716156No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT31100.17469976637417095No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA31050.1744188985825726No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA29720.16694781532605663No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28380.15942055851122094No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA27500.15447728537909006No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC25590.14374813574003326No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC21790.122402183578559No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21460.12054845615400993No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20730.11644778639667408No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG20540.11538048878860037No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG19110.10734766994888768No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18840.10583098387425662No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.10038214871724871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50650.030.0236931
ATTGGAC6400.020.2343753
GTATTGG6750.018.3629651
GTATCAA85500.017.8292392
CGGGCGT1401.87174E-917.1785726
TCCGATC650.001580394917.07692331
TTGGACC10650.017.0234744
TATACTG3050.016.9836065
GGCGTCG1201.0421172E-716.9583328
TTGCGTG904.4481625E-516.4444457
GGACCCT10900.016.293586
GACCGTC1151.2430555E-616.08695622
GACCCTC10500.016.0333337
CGGGATA1504.6748028E-916.03333324
TGGACCC11200.015.8571435
GTATACG1751.3278623E-1015.8571431
TATTGGA8100.015.7592592
TGCGTGC1659.749783E-1015.6969718
ACGGACC1101.45267895E-515.1363648
ACGTTTA1353.9754013E-715.07407526
Produced by FastQC (version 0.11.5)