##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088155_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1780197
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.819286292472125	31.0	31.0	33.0	28.0	34.0
2	30.976247572600112	31.0	31.0	34.0	28.0	34.0
3	31.02648358580539	31.0	31.0	34.0	27.0	34.0
4	34.99207166397876	35.0	35.0	37.0	32.0	37.0
5	34.82951268876422	35.0	35.0	37.0	32.0	37.0
6	34.91124577785492	35.0	35.0	37.0	32.0	37.0
7	34.73620616145292	35.0	35.0	37.0	31.0	37.0
8	34.80409078321107	35.0	35.0	37.0	32.0	37.0
9	36.25163507184879	38.0	35.0	39.0	32.0	39.0
10	36.06557532677563	38.0	35.0	39.0	31.0	39.0
11	36.12001424561439	38.0	35.0	39.0	31.0	39.0
12	35.946622199677904	37.0	35.0	39.0	30.0	39.0
13	35.99412143712185	38.0	35.0	39.0	30.0	39.0
14	36.848419023287875	38.0	36.0	40.0	31.0	41.0
15	36.90849102655493	38.0	36.0	40.0	31.0	41.0
16	36.87730964606726	38.0	36.0	40.0	31.0	41.0
17	36.974728077847566	38.0	36.0	40.0	31.0	41.0
18	36.97701209472884	38.0	36.0	40.0	31.0	41.0
19	37.087511663035045	39.0	36.0	40.0	31.0	41.0
20	37.044642250267806	39.0	36.0	40.0	31.0	41.0
21	36.94055657885054	39.0	36.0	40.0	31.0	41.0
22	36.8844088603677	39.0	36.0	40.0	31.0	41.0
23	36.7539547589396	39.0	35.0	40.0	30.0	41.0
24	36.676988558007906	38.0	35.0	40.0	30.0	41.0
25	36.58106939849915	38.0	35.0	40.0	30.0	41.0
26	36.30359954544357	38.0	35.0	40.0	30.0	41.0
27	36.11756564020723	38.0	35.0	40.0	29.0	41.0
28	35.97057291973866	38.0	34.0	40.0	29.0	41.0
29	35.818903188804384	38.0	34.0	40.0	29.0	41.0
30	35.63455224337531	38.0	34.0	40.0	28.0	41.0
31	35.35601902486073	38.0	34.0	40.0	27.0	41.0
32	35.07890138001581	37.0	33.0	40.0	26.0	41.0
33	35.251903581457555	38.0	34.0	40.0	27.0	41.0
34	35.226882193375225	38.0	34.0	40.0	27.0	41.0
35	35.14153152712874	38.0	33.0	40.0	26.0	41.0
36	35.03739361430224	38.0	33.0	40.0	25.0	41.0
37	34.89984254551603	38.0	33.0	40.0	25.0	41.0
38	34.78458957070482	38.0	33.0	40.0	25.0	41.0
39	34.72964508984118	38.0	33.0	40.0	24.0	41.0
40	34.57289502229248	38.0	33.0	40.0	24.0	41.0
41	34.43400084372685	38.0	33.0	40.0	23.0	41.0
42	34.20943468616114	38.0	33.0	40.0	22.0	41.0
43	33.881916439585055	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	2.0
14	7.0
15	10.0
16	26.0
17	77.0
18	191.0
19	437.0
20	893.0
21	1739.0
22	3154.0
23	5454.0
24	8704.0
25	13582.0
26	19828.0
27	28316.0
28	38843.0
29	51192.0
30	63730.0
31	77469.0
32	93216.0
33	110892.0
34	133147.0
35	157388.0
36	196398.0
37	248675.0
38	301181.0
39	225644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.93063689018687	23.73203639821885	14.417730172559553	21.919596539034725
2	18.242587758545824	24.040260712718872	35.58521893925223	22.131932589483075
3	20.499023422688612	26.583630912758533	32.09324585986832	20.824099804684536
4	13.601921585082998	16.61546446825829	35.577523161762436	34.20509078489628
5	11.062258839892438	41.069162570209926	34.76536585557666	13.103212734320977
6	31.380515751908355	37.11038722118957	15.295498194862702	16.213598832039374
7	24.114465983259155	35.372714368128925	21.901003091230915	18.611816557381008
8	27.426121940436932	34.24632217670292	20.327076160672107	18.00047972218805
9	25.04649766289911	13.632142959458982	23.74518101086565	37.57617836677626
10	16.05985180291844	28.071443778413286	34.185654733717676	21.683049684950596
11	31.742666682395264	22.019248431493818	25.102727394777098	21.135357491333824
12	19.87684509073996	27.583576424406964	32.243229260581835	20.296349224271246
13	30.945563889839157	21.066095493925673	28.991566663689465	18.996773952545702
14	20.603506241163196	22.51284548844875	29.04240373396877	27.841244536419286
15	22.766581451378695	29.30518363978818	27.11565068360412	20.812584225229006
16	21.422123506555735	28.524820567611336	27.513640344298974	22.53941558153395
17	19.227310235889625	27.2918109624946	28.572455745066417	24.90842305654936
18	20.157600535221665	26.404830476627023	32.94562343381098	20.491945554340337
19	19.646814369420913	24.99835692341915	34.23873874632976	21.116089960830177
20	23.679457947631636	24.111825826018134	32.92388426674127	19.284831959608965
21	23.29433203179199	24.831577628768052	31.66233849399814	20.211751845441825
22	21.65237892210806	24.514983454078397	32.44803805421535	21.384599569598194
23	20.649568558985326	26.51830106443276	33.292158115085016	19.539972261496903
24	19.52884989694961	25.65334061342649	32.05735095610205	22.760458533521852
25	20.618279887001272	26.484709276557595	33.02516519239163	19.871845644049507
26	21.93077507714034	25.546217637710882	31.732892483247642	20.79011480190114
27	20.662713171632127	26.832479776114663	31.452249385882574	21.05255766637063
28	20.407572869744193	26.407133592518132	31.633746152813423	21.551547384924252
29	19.6353549635237	26.152386505538434	32.43461257377695	21.77764595716092
30	18.986999753398077	26.584698210366604	34.08471084941723	20.343591186818088
31	20.393192438814356	25.887022616036315	33.15694836020957	20.562836584939756
32	21.248996599814514	26.18412456598905	31.21418584572382	21.352692988472626
33	22.074354692205414	26.117952114288475	30.749911386211753	21.05778180729436
34	20.52806515233988	28.59402639146117	30.932194582959077	19.945713873239875
35	19.36555336291433	29.344729824845228	31.107568432033084	20.18214838020736
36	20.29780973678756	26.140870926082897	32.875069444561476	20.68624989256807
37	20.226750185513175	26.459150307522144	32.10099781091643	21.21310169604825
38	20.62855964817377	26.070710151741633	32.529602060895506	20.77112813918909
39	21.369095667501966	25.689404037867718	32.48882005755543	20.452680237074887
40	19.840107583598893	26.330400511853462	31.685257305792565	22.14423459875508
41	20.686755454592948	25.869777333632175	31.5762806026524	21.867186609122474
42	18.44689099015446	27.428312709211394	31.86192314670792	22.262873153926222
43	19.17518117376897	27.219627940053826	31.448373410358517	22.156817475818688
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	175.0
1	247.5
2	320.0
3	674.5
4	1029.0
5	1029.0
6	2068.5
7	3108.0
8	3325.5
9	3543.0
10	5571.0
11	7599.0
12	7599.0
13	13966.5
14	20334.0
15	34142.5
16	47951.0
17	46622.0
18	45293.0
19	45293.0
20	48414.0
21	51535.0
22	42314.5
23	33094.0
24	35970.5
25	38847.0
26	38847.0
27	43188.5
28	47530.0
29	50219.5
30	52909.0
31	56495.5
32	60082.0
33	60082.0
34	64944.0
35	69806.0
36	75078.5
37	80351.0
38	83664.5
39	86978.0
40	86978.0
41	91270.5
42	95563.0
43	106791.5
44	118020.0
45	144812.0
46	171604.0
47	171604.0
48	174016.0
49	176428.0
50	159010.0
51	141592.0
52	132348.0
53	123104.0
54	123104.0
55	106718.0
56	90332.0
57	75223.5
58	60115.0
59	52518.0
60	44921.0
61	44921.0
62	39459.5
63	33998.0
64	29532.0
65	25066.0
66	21288.5
67	17511.0
68	17511.0
69	14689.5
70	11868.0
71	9987.5
72	8107.0
73	6521.5
74	4936.0
75	4936.0
76	4002.0
77	3068.0
78	2365.0
79	1662.0
80	1260.5
81	859.0
82	859.0
83	663.5
84	468.0
85	360.0
86	252.0
87	191.5
88	131.0
89	131.0
90	106.5
91	82.0
92	49.5
93	17.0
94	12.0
95	7.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1780197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.07837978370533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.41512894322705	37.21993970336989
2	12.652914680127312	12.166632747239627
3	4.322757865134805	6.234935830588513
4	1.8349132738454483	3.5287862900041427
5	0.9612057300003837	2.3106607068616083
6	0.579812967747076	1.672588084011673
7	0.3576805455852044	1.203769077832187
8	0.26836574286863596	1.0322072085259595
9	0.19334782650712212	0.8366265209847047
>10	1.2257993299737053	11.318659135067787
>50	0.11626924621668035	3.8347091466160386
>100	0.05948488563600911	5.4300279314993825
>500	0.005396879085787168	1.879714129250425
>1k	0.005748849460947201	4.978895902249047
>5k	7.039407503200654E-4	2.084979995183155
>10k+	4.6929383354671025E-4	4.266867590715974
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23461	1.317887851737757	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22710	1.2757015094396857	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19214	1.0793187495541223	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10259	0.5762845348014854	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7751	0.43540125053575535	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6365	0.3575446987046939	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6100	0.34265870574998164	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5802	0.3259189853707202	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5800	0.32580663825408085	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5145	0.2890129575546976	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4442	0.24952294605597022	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4334	0.24345620175744595	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4274	0.24008578825826582	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3501	0.19666362767716156	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3110	0.17469976637417095	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3105	0.1744188985825726	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2972	0.16694781532605663	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2838	0.15942055851122094	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2750	0.15447728537909006	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2559	0.14374813574003326	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2179	0.122402183578559	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2146	0.12054845615400993	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2073	0.11644778639667408	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2054	0.11538048878860037	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1911	0.10734766994888768	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1884	0.10583098387425662	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1787	0.10038214871724871	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	5.6173558319669115E-5	0.0	0.0	0.0	0.0
5	5.6173558319669115E-5	0.0	0.0	0.0	0.0
6	1.1234711663933823E-4	0.0	0.0	0.0	0.0
7	1.1234711663933823E-4	0.0	0.0	0.0	0.0
8	1.6852067495900735E-4	0.0	0.0	0.0	0.0
9	1.6852067495900735E-4	0.0	0.0	0.0	0.0
10	1.6852067495900735E-4	0.0	0.0	0.0	0.0
11	1.6852067495900735E-4	0.0	0.0	1.6852067495900735E-4	0.0
12	1.6852067495900735E-4	0.0	0.0	3.370413499180147E-4	0.0
13	1.6852067495900735E-4	0.0	0.0	3.370413499180147E-4	0.0
14	1.6852067495900735E-4	0.0	0.0	3.370413499180147E-4	0.0
15	1.6852067495900735E-4	0.0	0.0	7.302562581556985E-4	0.0
16	1.6852067495900735E-4	0.0	0.0	0.001460512516311397	0.0
17	1.6852067495900735E-4	0.0	0.0	0.0026401572410244484	0.0
18	1.6852067495900735E-4	0.0	0.0	0.003370413499180147	0.0
19	1.6852067495900735E-4	0.0	0.0	0.004213016873975184	0.0
20	1.6852067495900735E-4	0.0	0.0	0.005392661598688235	0.0
21	1.6852067495900735E-4	0.0	0.0	0.008482207306270036	0.0
22	1.6852067495900735E-4	0.0	0.0	0.014380430929835293	0.0
23	1.6852067495900735E-4	0.0	0.0	0.024997233452252757	0.0
24	1.6852067495900735E-4	0.0	0.0	0.03758011051585864	0.0
25	1.6852067495900735E-4	0.0	0.0	0.0427480778812682	0.0
26	1.6852067495900735E-4	0.0	0.0	0.050556202487702206	0.0
27	1.6852067495900735E-4	0.0	0.0	0.10740384350720734	0.0
28	1.6852067495900735E-4	0.0	0.0	0.16958797256708105	0.0
29	1.6852067495900735E-4	0.0	0.0	0.2455346234152737	0.0
30	1.6852067495900735E-4	0.0	0.0	0.39928165253620806	0.0
31	1.6852067495900735E-4	0.0	0.0	0.624930836306319	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5065	0.0	30.023693	1
ATTGGAC	640	0.0	20.234375	3
GTATTGG	675	0.0	18.362965	1
GTATCAA	8550	0.0	17.829239	2
CGGGCGT	140	1.87174E-9	17.178572	6
TCCGATC	65	0.0015803949	17.076923	31
TTGGACC	1065	0.0	17.023474	4
TATACTG	305	0.0	16.983606	5
GGCGTCG	120	1.0421172E-7	16.958332	8
TTGCGTG	90	4.4481625E-5	16.444445	7
GGACCCT	1090	0.0	16.29358	6
GACCGTC	115	1.2430555E-6	16.086956	22
GACCCTC	1050	0.0	16.033333	7
CGGGATA	150	4.6748028E-9	16.033333	24
TGGACCC	1120	0.0	15.857143	5
GTATACG	175	1.3278623E-10	15.857143	1
TATTGGA	810	0.0	15.759259	2
TGCGTGC	165	9.749783E-10	15.696971	8
ACGGACC	110	1.45267895E-5	15.136364	8
ACGTTTA	135	3.9754013E-7	15.074075	26
>>END_MODULE