FastQCFastQC Report
Wed 25 May 2016
SRR2088154_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088154_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492225
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT361531.4506314638525815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT353911.4200563753272677No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT318361.2774127536638946No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG196330.7877699645898745No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116340.46681178465026235No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT102680.41200132411800705No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102600.41168032581327935No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT102000.4092728385278215No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA101710.40810921967318364No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA84700.3398569551304557No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT80070.32127917824433994No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT75470.30282177572249697No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG73780.2960406865351243No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60560.2429957166788713No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58170.23340589232513118No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54240.21763685060538274No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT53600.2150688641675611No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA43670.17522494959323495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43420.17422182989096088No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC42250.16952722968431821No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36220.14533198246546758No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG36020.14452948670364835No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35350.14184112590155382No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35190.1411991292920984No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34010.1364644042973648No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28560.11459639478779003No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA28110.1127907793236967No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC26690.10709305941477996No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26420.10600969013632397No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA25980.10424419946032161No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA25130.10083359247258976No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA25100.10071321810831686No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC24970.10019159586313435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76900.030.9856971
GTATTGG8500.024.5941181
ATTGGAC8850.024.0395473
TATATCG603.7285354E-521.5833345
TATTGGA10750.020.6511612
TAATACG450.003827043820.5555554
GGACCCT14900.020.1140946
TTGGACC15550.019.2733144
CGACGAG1103.856985E-818.524
TGGACCC15900.018.2672945
CGTCTGT6700.018.22387934
GACCCTC15350.018.1986967
AGCCGTC1502.5102054E-1017.26666612
GTATCAA141050.016.9457632
TATACCG1655.4569682E-1116.8181825
CTGGTCG700.00259363315.8571439
ACGTGCG957.064969E-515.5789489
GCGTTAT1101.4531432E-515.1363641
CGAATTA1353.9772567E-715.07407315
CCGAATT1353.9772567E-715.07407314
Produced by FastQC (version 0.11.5)