##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088154_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2492225
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.011823170058882	31.0	31.0	33.0	28.0	34.0
2	31.154973567795846	31.0	31.0	34.0	28.0	34.0
3	31.197897862351915	31.0	31.0	34.0	28.0	34.0
4	35.15644695001455	35.0	35.0	37.0	32.0	37.0
5	35.01970448093571	35.0	35.0	37.0	32.0	37.0
6	35.084934145191546	37.0	35.0	37.0	32.0	37.0
7	34.912282799506464	36.0	35.0	37.0	32.0	37.0
8	34.981421821866	37.0	35.0	37.0	32.0	37.0
9	36.44049754737233	38.0	35.0	39.0	32.0	39.0
10	36.28888764056215	38.0	35.0	39.0	32.0	39.0
11	36.327404628394305	38.0	35.0	39.0	32.0	39.0
12	36.171397446057235	38.0	35.0	39.0	31.0	39.0
13	36.21743221418612	38.0	35.0	39.0	32.0	39.0
14	37.11388658728646	39.0	36.0	41.0	31.0	41.0
15	37.15934115097954	39.0	36.0	41.0	31.0	41.0
16	37.12927644975875	39.0	36.0	40.0	31.0	41.0
17	37.24821153788282	39.0	36.0	40.0	32.0	41.0
18	37.26024576432706	39.0	36.0	40.0	32.0	41.0
19	37.36310164611943	39.0	36.0	41.0	32.0	41.0
20	37.3277200092287	39.0	36.0	41.0	31.0	41.0
21	37.22844245603828	39.0	36.0	41.0	31.0	41.0
22	37.178771980860475	39.0	36.0	40.0	31.0	41.0
23	37.07095547151641	39.0	36.0	40.0	31.0	41.0
24	36.98952141159005	39.0	36.0	40.0	31.0	41.0
25	36.89689975824815	39.0	35.0	40.0	31.0	41.0
26	36.62514740844025	38.0	35.0	40.0	30.0	41.0
27	36.447881712124705	38.0	35.0	40.0	30.0	41.0
28	36.29104234168263	38.0	35.0	40.0	30.0	41.0
29	36.14377514068754	38.0	35.0	40.0	30.0	41.0
30	35.96223816067971	38.0	35.0	40.0	29.0	41.0
31	35.695391467463814	38.0	34.0	40.0	29.0	41.0
32	35.4337975102569	38.0	34.0	40.0	27.0	41.0
33	35.54779724944578	38.0	34.0	40.0	28.0	41.0
34	35.518892154600806	38.0	34.0	40.0	27.0	41.0
35	35.397180029892965	38.0	34.0	40.0	27.0	41.0
36	35.293825798232504	38.0	34.0	40.0	26.0	41.0
37	35.13745909779414	38.0	34.0	40.0	25.0	41.0
38	34.998572761287605	38.0	34.0	40.0	25.0	41.0
39	34.91725185326365	38.0	33.0	40.0	24.0	41.0
40	34.72326013903239	38.0	33.0	40.0	24.0	41.0
41	34.57691259818034	38.0	33.0	40.0	23.0	41.0
42	34.345943484235974	38.0	33.0	40.0	22.0	41.0
43	34.021569882334056	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	4.0
15	13.0
16	23.0
17	95.0
18	218.0
19	478.0
20	963.0
21	1959.0
22	3756.0
23	6409.0
24	10667.0
25	16558.0
26	24898.0
27	35558.0
28	49619.0
29	66122.0
30	84060.0
31	101761.0
32	122366.0
33	147787.0
34	178562.0
35	212386.0
36	265245.0
37	343798.0
38	421554.0
39	397364.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.852571497356784	23.137437430408568	14.203412613227137	21.806578459007515
2	17.56486673554755	23.467744685973376	36.60764979084954	22.359738787629528
3	20.07671859482992	26.625244510427432	32.54224638626127	20.75579050848138
4	12.67040495942381	15.85663413215099	35.53154309903801	35.9414178093872
5	10.674798623719768	41.32941447902978	34.69112941247279	13.304657484777657
6	31.159987561315695	36.30374464584859	15.441101826680978	17.09516596615474
7	24.018337028157568	35.915296572339976	21.378968592322124	18.687397807180332
8	27.940294315320646	33.48453690978945	20.26133274483644	18.313836030053466
9	25.13565187733852	13.477314447933072	24.17831455827624	37.208719116452166
10	16.23184102558958	27.94165855811574	33.775321249084655	22.051179167210023
11	31.072555648065485	21.588339736580767	25.743502292128518	21.59560232322523
12	20.24672732197133	27.647343237468526	32.14019600958982	19.965733430970317
13	31.34801231830994	20.742669702775633	29.489713007453176	18.419604971461247
14	20.75851899407156	22.49536057137698	29.183239876014405	27.56288055853705
15	22.84408510467554	28.788010713318418	27.876696526196472	20.491207655809568
16	21.317417167390584	28.49028478568347	27.81502472690066	22.377273320025278
17	18.89917643872443	27.02199841507087	28.543129131599276	25.535696014605424
18	20.001484617159367	26.218820531854064	33.543279599554616	20.236415251431954
19	19.175596103883077	24.4413325442125	35.169778009609885	21.213293342294538
20	24.223615444030937	23.205970568467933	33.6703548034387	18.900059184062435
21	23.49065593997332	24.316062955792514	32.2561967719608	19.93708433227337
22	21.53011866906078	23.841988584497788	33.35208498430118	21.275807762140257
23	20.447271012849964	26.02738516787208	34.4748568046625	19.050487014615456
24	18.933362758177932	25.037025148210933	32.82596876285247	23.20364333075866
25	20.324408911715437	26.17905686685592	33.945129352285605	19.551404869143035
26	21.863716157249044	24.97756021226013	32.39615203282208	20.76257159766875
27	20.337008095176	26.63058110724353	32.01364242795093	21.018768369629548
28	19.994904151912447	25.9864578840193	32.20002206863345	21.818615895434803
29	19.07777187051731	25.91387214236275	33.20097503235061	21.807380954769332
30	18.387545265776563	26.161361835307805	35.369759953455244	20.08133294546038
31	20.013642427950927	25.64319032190111	33.90095196059746	20.442215289550504
32	21.313685562098126	25.881892686254247	31.597307626719097	21.207114124928527
33	22.350389712004333	25.74851789063989	31.02536889727051	20.875723500085268
34	20.171974841757866	28.899758248151752	31.26078102900019	19.66748588109019
35	18.77117836471426	29.918566742569393	31.408841497055846	19.901413395660505
36	19.94988413967439	25.864879776103685	33.76870065904964	20.416535425172285
37	20.036553681950867	26.324308599745205	32.67497918526618	20.964158533037747
38	20.518091263830513	25.892405380734086	33.12068533138059	20.468818024054812
39	21.478277442847254	25.3955000050156	32.86011495751788	20.266107594619264
40	19.52913561175255	26.444963837534736	31.720410476582174	22.305490074130546
41	20.76875081503476	25.827804471907633	31.587035680967812	21.816409032089798
42	18.022530068513078	27.794039462729085	32.14043675831837	22.042993710439468
43	18.900019059274342	27.5182617941799	31.511280081052075	22.070439065493687
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	146.0
1	246.5
2	347.0
3	1011.5
4	1676.0
5	1676.0
6	3158.0
7	4640.0
8	5095.0
9	5550.0
10	8313.5
11	11077.0
12	11077.0
13	20577.0
14	30077.0
15	50920.0
16	71763.0
17	70683.0
18	69603.0
19	69603.0
20	73611.0
21	77619.0
22	61971.0
23	46323.0
24	49797.5
25	53272.0
26	53272.0
27	57665.5
28	62059.0
29	65312.5
30	68566.0
31	73275.5
32	77985.0
33	77985.0
34	84745.5
35	91506.0
36	98471.5
37	105437.0
38	109043.5
39	112650.0
40	112650.0
41	119031.0
42	125412.0
43	144888.5
44	164365.0
45	212090.5
46	259816.0
47	259816.0
48	262998.5
49	266181.0
50	238977.5
51	211774.0
52	195387.0
53	179000.0
54	179000.0
55	154381.5
56	129763.0
57	104553.5
58	79344.0
59	68144.5
60	56945.0
61	56945.0
62	49134.0
63	41323.0
64	35531.0
65	29739.0
66	25125.0
67	20511.0
68	20511.0
69	17306.0
70	14101.0
71	12118.0
72	10135.0
73	8029.0
74	5923.0
75	5923.0
76	4621.5
77	3320.0
78	2586.0
79	1852.0
80	1463.5
81	1075.0
82	1075.0
83	819.0
84	563.0
85	459.5
86	356.0
87	292.5
88	229.0
89	229.0
90	192.0
91	155.0
92	91.5
93	28.0
94	19.5
95	11.0
96	11.0
97	9.0
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2492225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.77036081941598
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55674617151692	29.253783359434227
2	13.751404235729003	10.937966164571753
3	5.124231205046992	6.113775718404888
4	2.416998952384231	3.844996817458851
5	1.308536632523195	2.6020487010435502
6	0.7886158062721175	1.8818121098002059
7	0.5207838262456636	1.4498232475094253
8	0.35573369283064077	1.13181258555983
9	0.24849440685517782	0.8894441000013459
>10	1.6436223023825616	12.582587708985695
>50	0.16899205114863053	4.666256061552796
>100	0.09794536119645054	7.132378109105946
>500	0.00874432161756368	2.3363730631655413
>1k	0.007422505559094747	5.89222796972695
>5k	8.134252667501092E-4	2.186025432640427
>10k+	9.151034250938729E-4	7.098688851038612
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36153	1.4506314638525815	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35391	1.4200563753272677	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	31836	1.2774127536638946	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	19633	0.7877699645898745	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11634	0.46681178465026235	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10268	0.41200132411800705	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10260	0.41168032581327935	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	10200	0.4092728385278215	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10171	0.40810921967318364	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8470	0.3398569551304557	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	8007	0.32127917824433994	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7547	0.30282177572249697	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	7378	0.2960406865351243	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6056	0.2429957166788713	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5817	0.23340589232513118	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5424	0.21763685060538274	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	5360	0.2150688641675611	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4367	0.17522494959323495	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4342	0.17422182989096088	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	4225	0.16952722968431821	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3622	0.14533198246546758	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3602	0.14452948670364835	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3535	0.14184112590155382	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3519	0.1411991292920984	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3401	0.1364644042973648	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2856	0.11459639478779003	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	2811	0.1127907793236967	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	2669	0.10709305941477996	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2642	0.10600969013632397	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2598	0.10424419946032161	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	2513	0.10083359247258976	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	2510	0.10071321810831686	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	2497	0.10019159586313435	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.0124788090962895E-5	0.0
3	0.0	0.0	0.0	4.0124788090962895E-5	0.0
4	0.0	0.0	0.0	4.0124788090962895E-5	0.0
5	0.0	0.0	0.0	4.0124788090962895E-5	0.0
6	0.0	0.0	0.0	8.024957618192579E-5	0.0
7	0.0	0.0	0.0	8.024957618192579E-5	0.0
8	0.0	0.0	0.0	8.024957618192579E-5	0.0
9	0.0	0.0	0.0	8.024957618192579E-5	0.0
10	0.0	0.0	0.0	2.0062394045481448E-4	0.0
11	0.0	0.0	0.0	4.0124788090962896E-4	0.0
12	0.0	0.0	0.0	6.018718213644434E-4	0.0
13	0.0	0.0	0.0	6.419966094554063E-4	0.0
14	0.0	0.0	0.0	8.426205499102207E-4	0.0
15	0.0	0.0	0.0	0.0015648667355475528	0.0
16	0.0	0.0	0.0	0.0024074872854577736	0.0
17	0.0	0.0	0.0	0.0034908565639137717	0.0
18	0.0	0.0	0.0	0.004333477113823992	0.0
19	0.0	0.0	0.0	0.005416846392279991	0.0
20	0.0	0.0	0.0	0.006540340458826952	0.0
21	0.0	0.0	0.0	0.009028077320466652	0.0
22	0.0	4.0124788090962895E-5	0.0	0.014605422865110494	0.0
23	0.0	4.0124788090962895E-5	0.0	0.025840363530580106	0.0
24	0.0	4.0124788090962895E-5	0.0	0.04052603597187252	0.0
25	0.0	4.0124788090962895E-5	0.0	0.0468256277021537	0.0
26	0.0	4.0124788090962895E-5	0.0	0.05436908786325472	0.0
27	0.0	4.0124788090962895E-5	0.0	0.11130616216433106	0.0
28	0.0	4.0124788090962895E-5	0.0	0.1717340930293212	0.0
29	4.0124788090962895E-5	4.0124788090962895E-5	0.0	0.24584457663332965	0.0
30	4.0124788090962895E-5	4.0124788090962895E-5	0.0	0.39538966184834834	0.0
31	4.0124788090962895E-5	4.0124788090962895E-5	0.0	0.6003069546288958	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7690	0.0	30.985697	1
GTATTGG	850	0.0	24.594118	1
ATTGGAC	885	0.0	24.039547	3
TATATCG	60	3.7285354E-5	21.583334	5
TATTGGA	1075	0.0	20.651161	2
TAATACG	45	0.0038270438	20.555555	4
GGACCCT	1490	0.0	20.114094	6
TTGGACC	1555	0.0	19.273314	4
CGACGAG	110	3.856985E-8	18.5	24
TGGACCC	1590	0.0	18.267294	5
CGTCTGT	670	0.0	18.223879	34
GACCCTC	1535	0.0	18.198696	7
AGCCGTC	150	2.5102054E-10	17.266666	12
GTATCAA	14105	0.0	16.945763	2
TATACCG	165	5.4569682E-11	16.818182	5
CTGGTCG	70	0.002593633	15.857143	9
ACGTGCG	95	7.064969E-5	15.578948	9
GCGTTAT	110	1.4531432E-5	15.136364	1
CGAATTA	135	3.9772567E-7	15.074073	15
CCGAATT	135	3.9772567E-7	15.074073	14
>>END_MODULE