FastQCFastQC Report
Wed 25 May 2016
SRR2088153_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088153_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166475
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT222491.9073704965815814No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219051.8778799374182902No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT142961.2255727726697958No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71800.6155296941640412No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT55900.47922158640348056No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG55870.47896440129449835No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT51570.4421012023403845No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA44630.3826057137958379No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA41750.35791594333354765No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39730.3405988126620802No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG34960.299706380333912No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT31900.2734734992177286No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA30040.25752802246083284No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT30010.25727083735185063No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT27840.23866778113547224No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA20150.1727426648663709No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19670.16862770312265585No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT19610.1681133329046915No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC19420.16648449388113762No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17610.1509676589725455No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG17090.14650978375018753No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16410.1406802546132579No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.13450781199768533No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA14950.1281639126427913No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC14550.12473477785636211No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT14490.12422040763839773No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA13710.1175335948048608No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC13570.11633339762961058No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13400.11487601534537818No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12770.10947512805675218No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG12140.10407424076812619No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12020.10304550033219745No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC11970.10261685848389378No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA11860.10167384641762574No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC11760.10081656272101845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47950.030.82691
TATACCG509.091242E-625.8999985
ATTCGTC502.7021364E-422.19999917
GTATTGG2600.022.0576921
TCGTCTC603.726348E-521.58333219
GCGACCG450.003825840820.55555718
GTCGACG450.003825840820.55555736
GTATCAA72750.020.3182132
TGCGATT609.2369283E-418.529
GTTATCA1409.458745E-1118.51
CGTGCGA500.00703494518.49999810
ATTGGAC3100.017.9032253
CGATCTG953.6064775E-617.52631633
TATACTG1705.456968E-1217.4117645
ATACTGA1401.869921E-917.1785726
GTTATAC650.00157995317.0769233
TTGGACC5550.016.6666684
GTGGTAT5950.016.1680661
CATATAG1151.2421569E-616.0869563
AATACTG1403.4726327E-815.8571435
Produced by FastQC (version 0.11.5)