FastQCFastQC Report
Wed 25 May 2016
SRR2088152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences552708
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132032.3887839510193447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124422.2510982290829875No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74101.3406717471069716No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42690.7723789053170933No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT23850.43151175665993613No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT22140.4005731778805445No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17150.31029042460033146No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA16310.2950925262525601No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG16280.29454974416871116No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15340.27754257220810985No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14930.2701245503955072No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT14300.2587261266346787No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG13730.24841326704154815No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13110.23719577064200267No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12600.22796847521657004No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA9990.1807464339217091No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9500.17188099321884248No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC9090.16446297140623983No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC8730.1579495864000521No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT8630.15614031278722218No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA8440.15270269292284533No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT8300.15016970986488345No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8230.1489032183359025No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7480.1353336662396781No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7350.1329816105429992No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC7120.12882028123349037No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA6860.12411616984013259No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC6650.12031669525318975No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC6420.11615536594368092No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6160.11145125455032313No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA6100.11036569038262518No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA6050.10946105357621022No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5820.10529972426670141No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5750.10403323273772047No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC5690.1029476685700225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC5600.10131932231847557No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC5570.1007765402346266No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTGC200.001841109837.08
GGTATCA25800.032.4108541
TGCGCGA250.005493905429.612
ACGTGCG250.005493905429.69
GCCTCGT250.005493905429.613
CCGTGGT400.001929926223.1259
CTCGCAC400.001929926223.12529
GTATCAA37350.022.3882182
GTGCACT450.003823332320.5555574
TCTATTC450.003823332320.5555573
ATTTAGC609.228548E-418.58
CGTGGTG500.007030374418.510
CATATAG500.007030374418.53
TCGTATG500.007030374418.537
TATAGTG500.007030374418.55
AACTCTA752.0648206E-417.2666667
CTACTAC650.00157853117.0769234
CCGAATT650.00157853117.07692314
TGGACTG700.002590211615.8571425
GTATACT700.002590211615.8571424
Produced by FastQC (version 0.11.5)