FastQCFastQC Report
Wed 25 May 2016
SRR2088151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172594
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT235382.0073444005342003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230251.9635952426841685No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT182581.557060670615746No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG81410.6942726979670714No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73940.6305677839047445No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT58780.5012817735720974No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT54960.4687044279605729No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA53590.4570209296653403No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA52660.44908979578609476No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA46320.3950216357920986No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG40120.3421474099304619No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT40100.34197684791155336No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT37440.31929209939672215No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32720.27903946293431486No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT29610.25251706899404225No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29460.2512378538522285No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA29290.24978807669150618No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26730.22795613827121747No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA26000.22173062458105702No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25090.21397005272072006No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC22590.19264980035715687No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22010.1877035018088102No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19250.16416594319943648No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG18840.1606694218118121No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18800.1603282977739951No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC17170.1464274932329519No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA17150.1462569312140434No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16460.14037254156169995No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTCTGTCTC15990.13636433411735008No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC15620.13320893676754272No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC15500.1321855646540917No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15090.12868904326646732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC14940.12740982812465354No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA14590.12442499279375469No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC14040.1197345372737708No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA13970.11913757020759104No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCT13970.11913757020759104No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA13940.11888172717922826No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTC13650.11640857790505493No Hit
CTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTAC13370.11402070964033587No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA13330.11367958560251885No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACCTGTCTCT13210.11265621348906783No Hit
ATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAG13180.11240037046070506No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA12430.10600429475163611No Hit
ATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGT12030.10259305437346601No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.10114327721274372No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACT11800.10063159115601819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44150.032.0554921
ATTGGAC2350.024.4042573
GTATTGG2700.021.9259261
CTTACTC603.7263693E-521.5833323
CGTCTGT4800.020.42708234
CGTGTCT1500.019.73333235
TTTTTAC11300.019.3185841
ACGTTTA701.2192241E-418.526
ACGCATA500.007034964418.57
GCGCACT500.007034964418.528
GTATCAA77550.018.3449382
GGACCCT6850.018.094896
GTGGTAT4500.018.0888881
TATTGGA3000.017.8833312
GACCCTC6750.017.8148147
TCTTACT953.6065103E-617.5263162
TAGAGGT852.7231914E-517.4117644
TATACCG852.7231914E-517.4117645
GCGTCTG5650.017.35398333
TTGGACC7500.017.2666684
Produced by FastQC (version 0.11.5)