FastQCFastQC Report
Wed 25 May 2016
SRR2088150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491877
Sequences flagged as poor quality0
Sequence length43
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94971.9307672446566928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88521.7996369010951925No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53721.0921429544377965No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29470.5991335232182029No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT19710.40070993358095625No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT17680.35943945335927474No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG17550.35679651620222125No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14670.2982452930305747No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA14100.2866570301111864No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT13030.26490362428005376No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG11870.2413204927248072No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA11820.24030397843363283No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11670.23725443556010953No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11020.2240397497748421No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9530.1937476238978444No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT8260.16792816090201412No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA8130.16528522374496063No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT7710.1567465036990955No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC7420.15085072081028386No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA7160.14556484649617688No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA6950.14129548647324433No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6570.13356997786031874No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG6250.12706428639680245No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA6110.12421804638151408No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC6090.12381144066504432No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC5660.1150694177609443No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5460.11100336059624663No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC5250.10673400057331407No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA5200.10571748628213964No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT5110.10388776055802569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18350.030.2452321
GTTCACC250.005493324729.616
CGCTATC358.862154E-426.4285710
CCTGTGC451.3217088E-424.6666688
GACCCTC1600.023.1250027
GACTTAC400.001929624723.1250027
TGTAGTG450.00382274320.5555575
GTATCAA27200.020.4044112
GGACCCT1900.019.4736846
TTGGACC2100.018.5000024
TCCGACC500.007029300618.58
ACTACAC609.2265784E-418.53
CAAGACG500.007029300618.54
TCTATAC609.2265784E-418.53
GTATTGG1258.543793E-917.761
TATGAGT752.0642382E-417.2666664
GTGGTAT2800.017.1785721
CATATGC650.001578196917.0769233
AGTGTGC650.001578196917.0769238
ATTGGAC1201.0376061E-716.9583323
Produced by FastQC (version 0.11.5)