##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088150_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491877
Sequences flagged as poor quality	0
Sequence length	43
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.342457565610914	31.0	30.0	31.0	27.0	34.0
2	30.50885282296184	31.0	30.0	31.0	27.0	34.0
3	30.47095107110111	31.0	30.0	33.0	27.0	34.0
4	34.41292640233229	35.0	35.0	37.0	30.0	37.0
5	34.181891001205585	35.0	35.0	37.0	30.0	37.0
6	34.42063767974514	35.0	35.0	37.0	30.0	37.0
7	34.22848191722728	35.0	35.0	37.0	30.0	37.0
8	34.3762749630497	35.0	35.0	37.0	30.0	37.0
9	35.71143395604999	37.0	35.0	39.0	30.0	39.0
10	35.45284288551813	37.0	34.0	39.0	30.0	39.0
11	35.623350959691145	37.0	35.0	39.0	30.0	39.0
12	35.37045033616128	37.0	34.0	39.0	30.0	39.0
13	35.47665981536034	37.0	35.0	39.0	30.0	39.0
14	36.166389158265176	38.0	34.0	40.0	29.0	41.0
15	36.274527981588896	38.0	34.0	40.0	30.0	41.0
16	36.16843235199044	38.0	34.0	40.0	29.0	41.0
17	36.3149425567774	38.0	35.0	40.0	30.0	41.0
18	36.3298121278287	38.0	35.0	40.0	30.0	41.0
19	36.450667544935015	38.0	35.0	40.0	30.0	41.0
20	36.443667827525985	38.0	35.0	40.0	30.0	41.0
21	36.35905521095721	38.0	35.0	40.0	30.0	41.0
22	36.29102804156324	38.0	35.0	40.0	30.0	41.0
23	36.146048707298775	38.0	34.0	40.0	29.0	41.0
24	36.04570858161695	38.0	34.0	40.0	29.0	41.0
25	35.93225542157897	38.0	34.0	40.0	29.0	41.0
26	35.61270398900538	38.0	34.0	40.0	27.0	41.0
27	35.37718982591176	38.0	34.0	40.0	27.0	41.0
28	35.19178778434446	37.0	33.0	40.0	27.0	41.0
29	35.006210902319076	37.0	33.0	40.0	27.0	41.0
30	34.7658317018279	37.0	33.0	40.0	26.0	41.0
31	34.46878182960374	37.0	33.0	39.0	25.0	40.0
32	34.195282560477516	36.0	33.0	39.0	25.0	40.0
33	34.329328267026106	36.0	33.0	39.0	25.0	40.0
34	34.257125256923985	36.0	33.0	39.0	25.0	40.0
35	34.106874279545494	36.0	33.0	39.0	24.0	40.0
36	33.95098571390815	36.0	33.0	39.0	24.0	40.0
37	33.76329448215712	36.0	33.0	39.0	23.0	40.0
38	33.63508966672563	36.0	32.0	39.0	22.0	40.0
39	33.49566050049098	36.0	32.0	39.0	21.0	41.0
40	33.260496018313525	36.0	31.0	39.0	19.0	41.0
41	33.06245667107834	36.0	31.0	40.0	18.0	41.0
42	32.77454729536043	36.0	31.0	39.0	16.0	41.0
43	32.396643876416256	36.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	0.0
15	7.0
16	15.0
17	45.0
18	108.0
19	215.0
20	459.0
21	852.0
22	1422.0
23	2299.0
24	3545.0
25	5435.0
26	7554.0
27	10394.0
28	13686.0
29	17471.0
30	21565.0
31	25558.0
32	30198.0
33	35545.0
34	41759.0
35	49240.0
36	60559.0
37	70204.0
38	66495.0
39	27244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.1318906962513	26.382408610282653	16.11459775512984	19.37110293833621
2	21.14979964503321	26.26937222110406	31.75936260487886	20.82146552898387
3	23.819979385090175	25.56940048020135	32.116972332514024	18.493647802194452
4	17.08658872035082	19.051714148049207	32.62339975237712	31.238297379222857
5	10.804530400892906	42.930041453452795	33.63666119781978	12.628766947834519
6	31.72419121040423	37.42216041815332	14.076486601325128	16.777161770117328
7	22.34583036002903	37.52076230439724	21.714981590926186	18.418425744647543
8	27.467232661823992	35.63573820284339	20.03712310191369	16.859906033418923
9	24.335962039290308	13.916893857610743	24.884676453666263	36.86246764943269
10	16.662295655214617	30.358605911640513	33.431731916719016	19.547366516425853
11	31.65811778147789	22.39157350313188	24.230447855866405	21.719860859523823
12	18.39118316164407	30.079064481567546	32.14767106410749	19.382081292680894
13	30.80099293115962	21.55315251577122	30.127653864685684	17.51820068838348
14	19.997072438841418	24.08020704363083	30.49115937520966	25.4315611423181
15	23.1370850842792	30.4838404723132	27.331426352523092	19.047648090884508
16	20.543550521776783	28.522984404637747	29.48603004409639	21.44743502948908
17	18.38162792730703	28.855994486426482	30.395607031839262	22.366770554427227
18	18.708132317632252	26.336258861463335	34.77678362680101	20.178825194103403
19	18.531665436684374	25.886756241906006	36.86287425514915	18.718704066260468
20	21.66273275635982	24.903990225198577	35.898812101399336	17.53446491704227
21	21.954675660785114	24.752326292955352	34.330737155833674	18.96226089042586
22	20.12454333095469	25.340684764687104	34.77739353537571	19.75737836898249
23	19.475803910327176	27.30967294669196	35.35375713847161	17.86076600450926
24	18.214106372121485	26.440756530596065	34.401893156215884	20.943243941066566
25	19.13750795422433	27.316178638155474	35.2482429550477	18.298070452572492
26	19.941774061401528	26.36126511302622	34.160979269207544	19.5359815563647
27	19.88362944394635	27.17813599741399	33.95909953098031	18.979135027659353
28	19.363377429723286	27.72075132604289	33.869036364782254	19.04683487945157
29	18.293191183974855	26.930513116083898	34.66395054048065	20.112345159460595
30	17.833320118647546	27.378389312775347	36.30216497213734	18.48612559643976
31	18.962667496142327	26.58001898848696	34.955893444905946	19.50142007046477
32	19.541267430678808	27.67114542863358	33.54436170018114	19.243225440506468
33	20.525659870252117	27.57254354238966	32.63661443816239	19.265182149195837
34	19.419692321454345	29.24369303708041	33.06659998332916	18.27001465813608
35	18.03194701114303	29.657617656446632	33.281287801625204	19.029147530785135
36	19.071637828156227	26.891478967302803	34.88087469021727	19.1560085143237
37	18.626201265763594	27.31007955240843	34.36997460747301	19.69374457435497
38	19.479463361775405	27.006548385063745	34.10730731463353	19.406680938527316
39	20.354072257901873	26.967717539140885	33.47137597407482	19.206834228882425
40	18.947419781774713	27.488376159080417	33.15727305810192	20.406931001042945
41	19.219439006092987	27.141338180073472	33.006015731575175	20.63320708225837
42	17.550525842842823	28.17432000276492	32.84459326213668	21.430560892255585
43	17.95794477074553	27.895591784124896	32.62807571811652	21.518387727013057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	91.0
1	88.0
2	85.0
3	177.5
4	270.0
5	270.0
6	615.5
7	961.0
8	1084.0
9	1207.0
10	1873.0
11	2539.0
12	2539.0
13	5100.0
14	7661.0
15	13075.5
16	18490.0
17	16884.0
18	15278.0
19	15278.0
20	17944.5
21	20611.0
22	16551.0
23	12491.0
24	13309.0
25	14127.0
26	14127.0
27	14939.0
28	15751.0
29	16126.0
30	16501.0
31	17333.5
32	18166.0
33	18166.0
34	19554.5
35	20943.0
36	22026.0
37	23109.0
38	23731.0
39	24353.0
40	24353.0
41	25186.0
42	26019.0
43	28308.5
44	30598.0
45	37256.0
46	43914.0
47	43914.0
48	44733.0
49	45552.0
50	41062.0
51	36572.0
52	33020.0
53	29468.0
54	29468.0
55	25242.0
56	21016.0
57	17296.5
58	13577.0
59	11799.5
60	10022.0
61	10022.0
62	8741.5
63	7461.0
64	6401.0
65	5341.0
66	4453.0
67	3565.0
68	3565.0
69	2979.0
70	2393.0
71	1961.0
72	1529.0
73	1248.5
74	968.0
75	968.0
76	752.5
77	537.0
78	425.5
79	314.0
80	244.5
81	175.0
82	175.0
83	136.5
84	98.0
85	77.0
86	56.0
87	45.5
88	35.0
89	35.0
90	30.0
91	25.0
92	16.0
93	7.0
94	3.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	491877.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.90283560294406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.74360036431216	47.652483243291016
2	9.8331732577586	11.190708826830072
3	2.732391385284853	4.664424533992937
4	1.1577733772227219	2.6352235259827954
5	0.5908721692799124	1.6811150955444893
6	0.3791314493692884	1.2944192721219916
7	0.25676138171434076	1.022731548501313
8	0.19846540458178463	0.9034595431831258
9	0.14371138852204646	0.7359826963806749
>10	0.8300321355975702	8.7460964891621
>50	0.07062907546137034	2.8147243374567372
>100	0.052702914133454945	6.118256347576504
>500	0.005736371606362443	2.180049594204819
>1k	0.00394375547937418	3.521006545609299
>5k	0.0010755696761929581	4.83931840016213
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9497	1.9307672446566928	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8852	1.7996369010951925	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5372	1.0921429544377965	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2947	0.5991335232182029	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1971	0.40070993358095625	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1768	0.35943945335927474	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1755	0.35679651620222125	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1467	0.2982452930305747	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1410	0.2866570301111864	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1303	0.26490362428005376	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1187	0.2413204927248072	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1182	0.24030397843363283	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1167	0.23725443556010953	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1102	0.2240397497748421	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	953	0.1937476238978444	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	826	0.16792816090201412	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	813	0.16528522374496063	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	771	0.1567465036990955	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	742	0.15085072081028386	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	716	0.14556484649617688	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	695	0.14129548647324433	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	657	0.13356997786031874	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	625	0.12706428639680245	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	611	0.12421804638151408	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	609	0.12381144066504432	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTCTGTCTC	566	0.1150694177609443	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	546	0.11100336059624663	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAC	525	0.10673400057331407	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	520	0.10571748628213964	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	511	0.10388776055802569	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	2.0330285823488392E-4	0.0	0.0	0.0	0.0
3	2.0330285823488392E-4	0.0	0.0	0.0	0.0
4	2.0330285823488392E-4	0.0	0.0	0.0	0.0
5	2.0330285823488392E-4	0.0	0.0	0.0	0.0
6	2.0330285823488392E-4	0.0	0.0	2.0330285823488392E-4	0.0
7	2.0330285823488392E-4	0.0	0.0	2.0330285823488392E-4	0.0
8	2.0330285823488392E-4	0.0	0.0	2.0330285823488392E-4	0.0
9	2.0330285823488392E-4	0.0	0.0	2.0330285823488392E-4	0.0
10	2.0330285823488392E-4	0.0	0.0	2.0330285823488392E-4	0.0
11	2.0330285823488392E-4	0.0	0.0	4.0660571646976783E-4	0.0
12	2.0330285823488392E-4	0.0	0.0	4.0660571646976783E-4	0.0
13	2.0330285823488392E-4	0.0	0.0	4.0660571646976783E-4	0.0
14	2.0330285823488392E-4	0.0	0.0	4.0660571646976783E-4	0.0
15	2.0330285823488392E-4	0.0	0.0	6.099085747046517E-4	0.0
16	2.0330285823488392E-4	0.0	0.0	0.0012198171494093035	0.0
17	2.0330285823488392E-4	0.0	0.0	0.0012198171494093035	0.0
18	2.0330285823488392E-4	0.0	0.0	0.0014231200076441875	0.0
19	2.0330285823488392E-4	0.0	0.0	0.0018297257241139554	0.0
20	2.0330285823488392E-4	0.0	0.0	0.0020330285823488394	0.0
21	2.0330285823488392E-4	0.0	0.0	0.003049542873523259	0.0
22	2.0330285823488392E-4	0.0	0.0	0.005285874314106982	0.0
23	2.0330285823488392E-4	0.0	0.0	0.009555234337039545	0.0
24	2.0330285823488392E-4	0.0	0.0	0.017687348666434902	0.0
25	2.0330285823488392E-4	0.0	0.0	0.020736891539958162	0.0
26	2.0330285823488392E-4	0.0	0.0	0.024193040129951188	0.0
27	2.0330285823488392E-4	0.0	0.0	0.07135930324044426	0.0
28	2.0330285823488392E-4	0.0	0.0	0.12218501779916524	0.0
29	2.0330285823488392E-4	0.0	0.0	0.18053293811257692	0.0
30	2.0330285823488392E-4	0.0	0.0	0.29275611585823286	0.0
31	2.0330285823488392E-4	0.0	0.0	0.46597015107435397	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1835	0.0	30.245232	1
GTTCACC	25	0.0054933247	29.6	16
CGCTATC	35	8.862154E-4	26.42857	10
CCTGTGC	45	1.3217088E-4	24.666668	8
GACCCTC	160	0.0	23.125002	7
GACTTAC	40	0.0019296247	23.125002	7
TGTAGTG	45	0.003822743	20.555557	5
GTATCAA	2720	0.0	20.404411	2
GGACCCT	190	0.0	19.473684	6
TTGGACC	210	0.0	18.500002	4
TCCGACC	50	0.0070293006	18.5	8
ACTACAC	60	9.2265784E-4	18.5	3
CAAGACG	50	0.0070293006	18.5	4
TCTATAC	60	9.2265784E-4	18.5	3
GTATTGG	125	8.543793E-9	17.76	1
TATGAGT	75	2.0642382E-4	17.266666	4
GTGGTAT	280	0.0	17.178572	1
CATATGC	65	0.0015781969	17.076923	3
AGTGTGC	65	0.0015781969	17.076923	8
ATTGGAC	120	1.0376061E-7	16.958332	3
>>END_MODULE