FastQCFastQC Report
Wed 25 May 2016
SRR2088149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3065274
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT375751.2258284251261062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT360391.1757187122586756No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT302200.9858825018579089No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG230230.7510910933247729No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178150.5811878481336415No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT118470.3864907345966462No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT100700.32851875558269833No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA98570.3215699477436601No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA98100.3200366427275343No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG86340.2816713938134079No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA85950.28039907688513327No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT74850.24418697969577924No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT73420.239521817625439No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA63500.20715929473189018No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA58300.19019506902156216No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA53260.17375281948693658No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48050.15675597026562715No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44240.14432641258171375No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT44050.14370656587306713No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42780.13956337997842932No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC42680.13923714486861535No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38980.12716644580549732No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG37390.12197930755945471No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35100.11450852354471411No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT33710.10997385551829951No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC32950.1074944686837131No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32490.1059937871785687No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG31980.10432998811851729No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC31740.1035470238549637No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA30690.10012155520191669No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA100100.025.449051
TAAGACG450.003827240820.5555574
GTACCGA555.145784E-420.1818186
GTATTGG15400.018.740261
CTAGACG500.007037490618.54
ATTGGAC14800.018.53
ACGTTTA1705.456968E-1217.41176426
TCACGTT1757.2759576E-1216.91428624
TTGGACC19850.016.0302284
AACCCGC1403.479181E-815.8571436
CGCGATA700.002593815815.85714314
GTATCAA160550.015.8439742
TATTGGA17700.015.5734462
TGCGACG1802.0190782E-1015.41666722
GACGTAT855.367776E-415.23529328
TGGACCC21400.015.2149545
CGTCTGT8650.015.18497134
CAATGCG1954.1836756E-1115.17948719
CGAACTA750.004106481614.824
GGACCCT21950.014.6651486
Produced by FastQC (version 0.11.5)