##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088149_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3065274
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.675610075967107	31.0	31.0	34.0	30.0	34.0
2	31.841826211947122	31.0	31.0	34.0	30.0	34.0
3	31.937762170690124	31.0	31.0	34.0	30.0	34.0
4	35.627912545501644	37.0	35.0	37.0	33.0	37.0
5	35.536716130434016	37.0	35.0	37.0	33.0	37.0
6	35.56662764894753	37.0	35.0	37.0	33.0	37.0
7	35.42672400574957	37.0	35.0	37.0	33.0	37.0
8	35.49475283449375	37.0	35.0	37.0	33.0	37.0
9	37.001282103981566	39.0	37.0	39.0	33.0	39.0
10	36.882827440548546	39.0	37.0	39.0	33.0	39.0
11	36.959478010774895	39.0	37.0	39.0	33.0	39.0
12	36.84930547807472	39.0	37.0	39.0	32.0	39.0
13	36.863989320367445	39.0	37.0	39.0	33.0	39.0
14	37.89679193442413	40.0	37.0	41.0	33.0	41.0
15	37.897040199342705	40.0	37.0	41.0	33.0	41.0
16	37.77900474802579	40.0	37.0	41.0	32.0	41.0
17	37.80970510303484	40.0	37.0	41.0	32.0	41.0
18	37.79311572146568	40.0	37.0	41.0	32.0	41.0
19	37.83206199511039	40.0	37.0	41.0	32.0	41.0
20	37.75356297675183	40.0	37.0	41.0	32.0	41.0
21	37.67513507764722	40.0	37.0	41.0	32.0	41.0
22	37.573059047902404	39.0	36.0	41.0	32.0	41.0
23	37.35304511113851	39.0	36.0	41.0	31.0	41.0
24	37.20805089528701	39.0	36.0	41.0	31.0	41.0
25	37.102352024647715	39.0	36.0	41.0	31.0	41.0
26	37.235806325959764	39.0	36.0	41.0	31.0	41.0
27	37.14035743623572	39.0	36.0	41.0	31.0	41.0
28	37.05849395518965	39.0	36.0	41.0	31.0	41.0
29	36.97652053291158	39.0	36.0	41.0	31.0	41.0
30	36.77688160993112	39.0	35.0	40.0	30.0	41.0
31	36.590525675681846	39.0	35.0	40.0	30.0	41.0
32	36.429958953098485	38.0	35.0	40.0	30.0	41.0
33	36.216794974935354	38.0	35.0	40.0	30.0	41.0
34	36.082758017717175	38.0	35.0	40.0	30.0	41.0
35	35.8583235952153	38.0	35.0	40.0	29.0	41.0
36	35.755581393376254	38.0	35.0	40.0	28.0	41.0
37	35.58057648353785	38.0	35.0	40.0	27.0	41.0
38	35.44581724178654	38.0	34.0	40.0	26.0	41.0
39	35.3258129615819	38.0	34.0	40.0	25.0	41.0
40	35.097633360019366	38.0	34.0	40.0	24.0	41.0
41	34.920129489239784	38.0	34.0	40.0	24.0	41.0
42	34.69452649257456	38.0	33.0	40.0	23.0	41.0
43	34.36049795222222	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	6.0
13	13.0
14	11.0
15	34.0
16	44.0
17	115.0
18	229.0
19	438.0
20	1009.0
21	1998.0
22	3730.0
23	6420.0
24	10417.0
25	16439.0
26	24122.0
27	34259.0
28	47294.0
29	62023.0
30	79031.0
31	98858.0
32	123521.0
33	153721.0
34	191199.0
35	236129.0
36	307086.0
37	429534.0
38	577936.0
39	659653.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.314185942268125	22.453653409124275	13.46411446415557	22.768046184452025
2	17.776029157589175	22.390004939199564	36.23669531663401	23.597270586577253
3	19.813856771042325	26.022371898890604	31.696187681753734	22.467583648313333
4	13.104701243673485	15.427429978527204	35.03448631345844	36.433382464340866
5	11.158415202034142	41.15429811494829	34.09845253638011	13.588834146637463
6	31.99430132510177	36.041443603410336	15.435194374140778	16.529060697347123
7	25.711241474660994	34.8103954165272	21.004158192709692	18.474204916102117
8	28.533338292107004	33.95872603884677	19.451246446484067	18.05668922256216
9	25.86708398661914	13.644750844459583	22.788957854991104	37.699207313930174
10	16.347641352779554	28.142639124593753	32.973789618807324	22.535929903819365
11	31.822049187119976	21.680965551529816	24.606381028253917	21.89060423309629
12	20.22566335016054	27.266860972298073	31.81418692097346	20.69328875656793
13	31.165827263729117	20.720725129303286	28.32099185912907	19.79245574783853
14	20.998840560419723	21.974805514939284	28.21291016724769	28.813443757393305
15	23.482337957389777	28.39824433313303	26.569566048581628	21.549851660895566
16	22.116261058554635	28.180743385420033	26.705508218841125	22.997487337184214
17	19.86683735287612	26.795777473726655	27.56494199213512	25.7724431812621
18	20.923219261964835	26.384231882696298	31.48093775629846	21.21161109904041
19	20.323370765549832	24.419546180863442	32.807670700890036	22.449412352696694
20	24.894577124263606	23.495289491249398	31.481166120875326	20.12896726361167
21	23.81552187504282	24.649346192216424	30.39297628857975	21.14215564416101
22	22.332489689339354	23.972962939039057	31.60001357138057	22.094533800241024
23	21.28661255078665	26.01359617443661	32.54322452087481	20.156566753901934
24	20.19724827209574	25.087969297361347	30.833034828207857	23.88174760233506
25	21.31104756051172	26.203954361013075	31.771287004032917	20.713711074442283
26	22.86950530360418	24.93287712615577	30.48712774127207	21.710489828967983
27	21.407450035461757	26.63934773857084	29.99617652451298	21.957025701454423
28	21.209294829760733	25.76239513987983	30.32521725627138	22.70309277408806
29	20.203903468335945	25.791984664339957	31.64738290932556	22.356728957998534
30	19.676511789810633	25.937974875981723	33.27036995713923	21.115143377068414
31	21.023406064188716	25.517001090277734	32.07126018750689	21.388332658026656
32	22.217230825042066	25.599147090928902	30.206174064700253	21.977448019328776
33	23.18686681843124	25.44943779903526	29.798510671476674	21.565184711056826
34	21.271573112224225	28.130535802019658	29.971154291590246	20.626736794165872
35	20.051323307475936	29.00931531732563	30.06837235431482	20.870989020883616
36	20.962236981098588	25.376002275816127	32.33228089886907	21.32947984421621
37	21.090740990854325	25.83217682986904	31.331587323025612	21.745494856251025
38	21.229880265189998	25.314409087083245	32.17454622327401	21.281164424452754
39	22.219090365168007	24.720498069666856	32.12835133172434	20.932060233440794
40	20.65586958947226	25.435246571758345	30.9999367103887	22.908947128380692
41	21.592653707303164	25.054693316160314	31.03523534927057	22.317417627265947
42	19.247186385295407	26.969040940548872	31.176527775331014	22.607244898824703
43	20.137645117532724	26.458058888047205	30.769875710947865	22.634420283472213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	222.0
1	367.0
2	512.0
3	1097.0
4	1682.0
5	1682.0
6	3199.5
7	4717.0
8	5226.5
9	5736.0
10	9513.5
11	13291.0
12	13291.0
13	26019.0
14	38747.0
15	56329.0
16	73911.0
17	69802.5
18	65694.0
19	65694.0
20	70655.5
21	75617.0
22	59973.0
23	44329.0
24	49522.0
25	54715.0
26	54715.0
27	60404.0
28	66093.0
29	70924.5
30	75756.0
31	81549.0
32	87342.0
33	87342.0
34	95409.5
35	103477.0
36	114527.5
37	125578.0
38	134043.5
39	142509.0
40	142509.0
41	149306.5
42	156104.0
43	179011.0
44	201918.0
45	255571.5
46	309225.0
47	309225.0
48	312555.0
49	315885.0
50	288809.5
51	261734.0
52	245032.0
53	228330.0
54	228330.0
55	201086.0
56	173842.0
57	146041.5
58	118241.0
59	105298.0
60	92355.0
61	92355.0
62	81302.5
63	70250.0
64	61133.0
65	52016.0
66	44856.5
67	37697.0
68	37697.0
69	31933.0
70	26169.0
71	21750.0
72	17331.0
73	14017.5
74	10704.0
75	10704.0
76	8318.5
77	5933.0
78	4659.0
79	3385.0
80	2636.5
81	1888.0
82	1888.0
83	1422.0
84	956.0
85	749.0
86	542.0
87	436.5
88	331.0
89	331.0
90	323.0
91	315.0
92	207.0
93	99.0
94	81.5
95	64.0
96	64.0
97	40.0
98	16.0
99	16.0
100	16.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3065274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.11288879835339
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74584262521537	31.05650469816565
2	13.560406968688413	11.421358214655829
3	5.124305022570614	6.4739786275308004
4	2.5173585308701707	4.240529595044871
5	1.328940567708985	2.798276317377455
6	0.7978075326652281	2.0158787943371665
7	0.5521406913087725	1.6276567673892615
8	0.3702063561976714	1.247236728879691
9	0.27046632030235723	1.02511062635337
>10	1.4758469244198231	11.656361084731277
>50	0.14930451416263207	4.3966958455229115
>100	0.09300319411454955	7.245679752196979
>500	0.007534934714709015	2.1816748368722085
>1k	0.005592941231521169	4.898700901117415
>5k	6.991176539401461E-4	2.264700893396579
>10k+	5.437581752867803E-4	5.449656316428704
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37575	1.2258284251261062	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	36039	1.1757187122586756	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30220	0.9858825018579089	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23023	0.7510910933247729	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17815	0.5811878481336415	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11847	0.3864907345966462	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10070	0.32851875558269833	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9857	0.3215699477436601	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9810	0.3200366427275343	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	8634	0.2816713938134079	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8595	0.28039907688513327	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7485	0.24418697969577924	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7342	0.239521817625439	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6350	0.20715929473189018	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5830	0.19019506902156216	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5326	0.17375281948693658	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4805	0.15675597026562715	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4424	0.14432641258171375	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4405	0.14370656587306713	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4278	0.13956337997842932	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4268	0.13923714486861535	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3898	0.12716644580549732	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3739	0.12197930755945471	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3510	0.11450852354471411	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3371	0.10997385551829951	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3295	0.1074944686837131	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3249	0.1059937871785687	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	3198	0.10432998811851729	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3174	0.1035470238549637	No Hit
AACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAA	3069	0.10012155520191669	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	6.524702196280006E-5	0.0	0.0	0.0	0.0
3	6.524702196280006E-5	0.0	0.0	0.0	0.0
4	6.524702196280006E-5	0.0	0.0	3.262351098140003E-5	0.0
5	6.524702196280006E-5	0.0	0.0	3.262351098140003E-5	0.0
6	9.787053294420009E-5	0.0	0.0	3.262351098140003E-5	0.0
7	9.787053294420009E-5	0.0	0.0	3.262351098140003E-5	0.0
8	1.6311755490700015E-4	0.0	0.0	3.262351098140003E-5	0.0
9	1.6311755490700015E-4	0.0	0.0	6.524702196280006E-5	0.0
10	1.6311755490700015E-4	0.0	0.0	1.6311755490700015E-4	0.0
11	1.6311755490700015E-4	0.0	0.0	3.9148213177680037E-4	0.0
12	1.6311755490700015E-4	0.0	0.0	6.524702196280006E-4	0.0
13	1.6311755490700015E-4	0.0	0.0	6.850937306094007E-4	0.0
14	1.6311755490700015E-4	0.0	0.0	8.482112855164009E-4	0.0
15	1.6311755490700015E-4	0.0	0.0	0.0015333050161258014	0.0
16	1.6311755490700015E-4	0.0	0.0	0.0029361159883260026	0.0
17	1.6311755490700015E-4	0.0	0.0	0.005252385268005405	0.0
18	1.6311755490700015E-4	0.0	0.0	0.0064268316633358065	0.0
19	1.6311755490700015E-4	0.0	0.0	0.007666525080629007	0.0
20	1.6311755490700015E-4	0.0	0.0	0.01017853542619681	0.0
21	1.6311755490700015E-4	0.0	0.0	0.013310392480411213	0.0
22	1.6311755490700015E-4	0.0	0.0	0.024271892170161624	0.0
23	1.6311755490700015E-4	0.0	0.0	0.039996424463196435	0.0
24	1.6311755490700015E-4	0.0	0.0	0.06136482415601346	6.524702196280006E-5
25	1.6311755490700015E-4	0.0	0.0	0.06906397274762387	6.524702196280006E-5
26	1.6311755490700015E-4	0.0	0.0	0.07735034453689947	6.524702196280006E-5
27	1.6311755490700015E-4	0.0	0.0	0.11512837025336072	6.524702196280006E-5
28	1.6311755490700015E-4	0.0	0.0	0.18608450663790577	6.524702196280006E-5
29	1.6311755490700015E-4	0.0	0.0	0.28056219444004027	6.524702196280006E-5
30	1.6311755490700015E-4	0.0	0.0	0.4404826452708632	6.524702196280006E-5
31	1.9574106588840019E-4	0.0	0.0	0.6413782258943246	6.524702196280006E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10010	0.0	25.44905	1
TAAGACG	45	0.0038272408	20.555557	4
GTACCGA	55	5.145784E-4	20.181818	6
GTATTGG	1540	0.0	18.74026	1
CTAGACG	50	0.0070374906	18.5	4
ATTGGAC	1480	0.0	18.5	3
ACGTTTA	170	5.456968E-12	17.411764	26
TCACGTT	175	7.2759576E-12	16.914286	24
TTGGACC	1985	0.0	16.030228	4
AACCCGC	140	3.479181E-8	15.857143	6
CGCGATA	70	0.0025938158	15.857143	14
GTATCAA	16055	0.0	15.843974	2
TATTGGA	1770	0.0	15.573446	2
TGCGACG	180	2.0190782E-10	15.416667	22
GACGTAT	85	5.367776E-4	15.235293	28
TGGACCC	2140	0.0	15.214954	5
CGTCTGT	865	0.0	15.184971	34
CAATGCG	195	4.1836756E-11	15.179487	19
CGAACTA	75	0.0041064816	14.8	24
GGACCCT	2195	0.0	14.665148	6
>>END_MODULE