FastQCFastQC Report
Wed 25 May 2016
SRR2088148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3411381
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT371041.0876533579802432No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT359811.0547341384618136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT299790.8787936615699038No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG237350.6957592834104429No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159760.4683147382247834No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA123380.3616717100787042No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT119330.34979968523011645No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT104840.3073242185496138No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA103870.304480795314273No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG92140.27009589371577086No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA87880.25760828239355266No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT77930.22844120900010878No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT75110.2201747620684995No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA68350.20035874034591855No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA60450.17720096348077216No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57440.16837755735873536No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49340.1446335076615599No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT47210.13838970200045084No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44650.13088540975047935No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42830.12555032697901525No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42170.12361562663331946No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41370.12127053530520338No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39490.11575957068413055No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG38040.11150909265192015No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC37560.11010203785505049No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36830.10796214201814457No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC35010.10262705924668045No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34420.10089755439219483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92750.026.9072781
CGCGTTA450.003827329520.55555535
GTATTGG15750.020.3206351
ATTGGAC15500.019.8129043
TTGGACC23300.017.388414
TATTGGA18700.016.9171122
TATACCG2000.016.6500025
GTATCAA151150.016.5478022
GTATCGT803.385007E-416.18756
TGGACCC23850.015.82395
CGTCTGT8550.015.57894734
GGACCCT24700.015.5040496
ACCGTGT1954.1836756E-1115.1794878
GCGTTAT1651.6483682E-814.5757571
TATACTG5500.014.4636365
TGGACTG5200.014.2307695
TAGACTG3600.013.8755
TCGTAGA950.001245952813.6315792
TAGAACG1102.4585825E-413.4545454
GACCCTC28500.013.2421057
Produced by FastQC (version 0.11.5)