##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3411381
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63158556608013	31.0	31.0	34.0	30.0	34.0
2	31.801738357574248	31.0	31.0	34.0	30.0	34.0
3	31.897871272660545	31.0	31.0	34.0	30.0	34.0
4	35.598564921361756	37.0	35.0	37.0	33.0	37.0
5	35.49483860055503	37.0	35.0	37.0	33.0	37.0
6	35.51705306443343	37.0	35.0	37.0	33.0	37.0
7	35.380980898938	37.0	35.0	37.0	33.0	37.0
8	35.43597123862741	37.0	35.0	37.0	33.0	37.0
9	36.94933576753813	39.0	37.0	39.0	33.0	39.0
10	36.81946226469574	39.0	37.0	39.0	32.0	39.0
11	36.8892545277118	39.0	37.0	39.0	33.0	39.0
12	36.77855449156808	39.0	37.0	39.0	32.0	39.0
13	36.79479864606152	39.0	37.0	39.0	32.0	39.0
14	37.81607272831736	40.0	37.0	41.0	32.0	41.0
15	37.82176455810711	40.0	37.0	41.0	32.0	41.0
16	37.700966265568105	39.0	37.0	41.0	32.0	41.0
17	37.71633364904125	39.0	37.0	41.0	32.0	41.0
18	37.695022924733415	39.0	37.0	41.0	32.0	41.0
19	37.73131262676318	40.0	37.0	41.0	32.0	41.0
20	37.646846541034265	39.0	37.0	41.0	32.0	41.0
21	37.56150896074053	39.0	36.0	41.0	32.0	41.0
22	37.46272404049856	39.0	36.0	41.0	32.0	41.0
23	37.24130315552558	39.0	36.0	41.0	31.0	41.0
24	37.102440331349676	39.0	36.0	41.0	31.0	41.0
25	36.992242731023005	39.0	36.0	40.0	31.0	41.0
26	37.13083176578635	39.0	36.0	41.0	31.0	41.0
27	37.042517971460825	39.0	36.0	41.0	31.0	41.0
28	36.96132328813463	39.0	36.0	41.0	31.0	41.0
29	36.88085763507506	39.0	35.0	41.0	30.0	41.0
30	36.69313307425937	39.0	35.0	40.0	30.0	41.0
31	36.518050314520714	39.0	35.0	40.0	30.0	41.0
32	36.37405584424607	38.0	35.0	40.0	30.0	41.0
33	36.17229737751368	38.0	35.0	40.0	30.0	41.0
34	36.031763089493666	38.0	35.0	40.0	29.0	41.0
35	35.819737226653956	38.0	35.0	40.0	28.0	41.0
36	35.73045930665616	38.0	35.0	40.0	27.0	41.0
37	35.573746233563476	38.0	34.0	40.0	27.0	41.0
38	35.46416597852893	38.0	34.0	40.0	26.0	41.0
39	35.35960070129956	38.0	34.0	40.0	25.0	41.0
40	35.15922056199528	38.0	34.0	40.0	25.0	41.0
41	35.00936688103733	38.0	34.0	40.0	24.0	41.0
42	34.792785385156336	38.0	33.0	40.0	23.0	41.0
43	34.47583867061463	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	5.0
13	11.0
14	12.0
15	29.0
16	54.0
17	106.0
18	229.0
19	571.0
20	1131.0
21	2297.0
22	4339.0
23	7478.0
24	12391.0
25	19151.0
26	27787.0
27	39129.0
28	54091.0
29	70798.0
30	91098.0
31	113069.0
32	139801.0
33	173302.0
34	215021.0
35	264572.0
36	341996.0
37	471121.0
38	637928.0
39	723862.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.90598499551941	22.536650113253252	13.51948082023087	23.037884070996466
2	17.830432895065076	22.533718749093108	36.20354923709782	23.432299118743995
3	19.708206148770834	25.75769167970391	31.89116079382514	22.642941377700115
4	13.246717385129367	15.55710136158934	35.30033731207391	35.89584394120739
5	11.326292782893496	40.70955428314809	34.516519849292706	13.44763308466571
6	32.076686831520725	36.31130618362475	15.32986201189489	16.28214497295963
7	25.651810806239467	34.598773927626375	21.05156240244054	18.69785286369362
8	28.005901422327206	34.21661198206826	19.72224738309793	18.055239212506606
9	25.68147621154014	13.80370588919854	22.6223925149375	37.89242538432382
10	16.325734358020988	27.90104652631881	33.39829236312215	22.374926752538048
11	32.20730841849679	21.74655953116934	24.411081611816446	21.63505043851742
12	20.06281327122359	27.136839889768982	31.786745602440767	21.013601236566657
13	31.097464633824252	20.768920270119345	28.075638575697052	20.05797652035935
14	20.96239030468892	22.103365176742205	27.738091992656344	29.196152525912527
15	23.401783617836884	28.770166686160238	26.019638381054477	21.808411314948405
16	22.318234169680842	28.407322430417477	26.152136041093033	23.122307358808648
17	20.045371654470724	26.90329810713022	27.44348989456176	25.60784034383729
18	21.144486646317137	26.529461235786915	31.045403606340074	21.280648511555878
19	20.58096120017084	24.724942772443185	32.03327332830897	22.660822699077002
20	24.872302448773677	23.92925328481339	30.869961461355388	20.32848280505754
21	23.926615057069263	24.92207701221294	29.731155798780613	21.420152131937183
22	22.455128875959616	24.370423590915234	30.888722191980317	22.285725341144833
23	21.45814261145266	26.22386652209179	31.915285920863134	20.402704945592415
24	20.473790526475934	25.336220140758243	30.184315384297445	24.005673948468377
25	21.53479778424046	26.335170419252496	31.044641451658432	21.085390344848612
26	23.0002160415386	25.236846895729325	29.888394172330795	21.874542890401276
27	21.614120498413985	26.76473252328016	29.415770328790597	22.205376649515255
28	21.499533473393914	25.913552312098826	29.804733039200254	22.78218117530701
29	20.554256472671916	25.80462281990783	30.929292271956722	22.711828435463527
30	19.911027235011275	26.067507557789643	32.575048052387	21.446417154812085
31	21.303220015588995	25.51221924493336	31.544966686511998	21.639594052965645
32	22.24571222035885	25.616898259092142	29.788405340828245	22.348984179720762
33	23.200985172866943	25.543995232429328	29.407357313650984	21.847662281052745
34	21.44530323643123	28.05494314472643	29.578666235169866	20.921087383672475
35	20.389425865946958	28.917291853357924	29.676808307251523	21.0164739734436
36	21.222285051127386	25.44298042347073	31.680073260653092	21.654661264748793
37	21.256347502668273	25.87389095501206	30.916570151501695	21.95319139081797
38	21.39925150547535	25.298288288525967	31.81726110334788	21.485199102650803
39	22.358511113241235	24.726555022731265	31.731254878889224	21.183678985138275
40	20.816349742230493	25.407774739907385	30.838449296633826	22.937426221228293
41	21.77379190421709	25.083096845529713	30.601683013418906	22.54142823683429
42	19.503626244034308	26.743568074043917	31.011575663931996	22.741230017989782
43	20.269093367173003	26.35662800490476	30.559207546738403	22.815071081183838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	205.0
1	382.5
2	560.0
3	1076.0
4	1592.0
5	1592.0
6	2991.0
7	4390.0
8	4932.5
9	5475.0
10	8964.0
11	12453.0
12	12453.0
13	24010.0
14	35567.0
15	54354.5
16	73142.0
17	69778.5
18	66415.0
19	66415.0
20	72751.0
21	79087.0
22	64598.5
23	50110.0
24	56243.0
25	62376.0
26	62376.0
27	69167.0
28	75958.0
29	81718.5
30	87479.0
31	94684.0
32	101889.0
33	101889.0
34	112061.0
35	122233.0
36	132768.0
37	143303.0
38	151283.0
39	159263.0
40	159263.0
41	168414.0
42	177565.0
43	201101.0
44	224637.0
45	280723.0
46	336809.0
47	336809.0
48	340747.0
49	344685.0
50	318668.5
51	292652.0
52	274638.5
53	256625.0
54	256625.0
55	226614.0
56	196603.0
57	166690.0
58	136777.0
59	121472.5
60	106168.0
61	106168.0
62	93956.0
63	81744.0
64	70723.5
65	59703.0
66	50870.0
67	42037.0
68	42037.0
69	35421.0
70	28805.0
71	23748.5
72	18692.0
73	15153.0
74	11614.0
75	11614.0
76	8987.5
77	6361.0
78	4969.5
79	3578.0
80	2832.5
81	2087.0
82	2087.0
83	1614.5
84	1142.0
85	877.0
86	612.0
87	495.5
88	379.0
89	379.0
90	379.5
91	380.0
92	251.5
93	123.0
94	93.0
95	63.0
96	63.0
97	44.5
98	26.0
99	21.5
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3411381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.07386468597982
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.60896099104576	31.706224254038723
2	13.599735389875997	11.715863238972995
3	5.36289396187576	6.930017065172842
4	2.5022370295541605	4.311240768930559
5	1.3440489332411831	2.8946690940883117
6	0.8208683502858432	2.1214783347129536
7	0.5133739531165675	1.5479100132894705
8	0.36325657938831124	1.2517491797491203
9	0.2617166472992264	1.0145832706651665
>10	1.381007496116762	11.228348939740536
>50	0.14000524836987918	4.237584680429615
>100	0.0893342146659973	7.313294935740286
>500	0.0061440677438479455	1.8218713495733938
>1k	0.0053248587108728745	4.852364100932525
>5k	4.778719355911554E-4	1.527023244784906
>10k+	6.144067743314855E-4	5.525777529178609
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37104	1.0876533579802432	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35981	1.0547341384618136	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29979	0.8787936615699038	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	23735	0.6957592834104429	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15976	0.4683147382247834	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12338	0.3616717100787042	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	11933	0.34979968523011645	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	10484	0.3073242185496138	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10387	0.304480795314273	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	9214	0.27009589371577086	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	8788	0.25760828239355266	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7793	0.22844120900010878	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	7511	0.2201747620684995	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6835	0.20035874034591855	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6045	0.17720096348077216	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5744	0.16837755735873536	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4934	0.1446335076615599	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4721	0.13838970200045084	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4465	0.13088540975047935	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4283	0.12555032697901525	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4217	0.12361562663331946	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4137	0.12127053530520338	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3949	0.11575957068413055	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3804	0.11150909265192015	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3756	0.11010203785505049	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3683	0.10796214201814457	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3501	0.10262705924668045	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3442	0.10089755439219483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.9313641601451142E-5	0.0	0.0	5.8627283202902284E-5	0.0
9	2.9313641601451142E-5	0.0	0.0	1.1725456640580457E-4	0.0
10	5.8627283202902284E-5	0.0	0.0	2.3450913281160914E-4	0.0
11	5.8627283202902284E-5	0.0	0.0	3.8107734081886484E-4	0.0
12	5.8627283202902284E-5	0.0	0.0	7.621546816377297E-4	0.0
13	5.8627283202902284E-5	0.0	0.0	8.794092480435342E-4	0.0
14	5.8627283202902284E-5	0.0	0.0	0.0011725456640580456	0.0
15	8.794092480435343E-5	0.0	0.0	0.001641563929681264	0.0
16	8.794092480435343E-5	0.0	0.0	0.002696855027333505	0.0
17	8.794092480435343E-5	0.0	0.0	0.0046901826562321825	0.0
18	8.794092480435343E-5	0.0	0.0	0.0050712599970510475	0.0
19	8.794092480435343E-5	0.0	0.0	0.005921355603493131	0.0
20	8.794092480435343E-5	0.0	0.0	0.007299096758761335	0.0
21	8.794092480435343E-5	0.0	0.0	0.010113206352500645	0.0
22	8.794092480435343E-5	0.0	0.0	0.018643476058522925	0.0
23	8.794092480435343E-5	0.0	0.0	0.029870600791878713	0.0
24	8.794092480435343E-5	0.0	0.0	0.04587584910627104	0.0
25	8.794092480435343E-5	0.0	0.0	0.05229553661698884	0.0
26	8.794092480435343E-5	0.0	0.0	0.05927218331813421	0.0
27	8.794092480435343E-5	0.0	0.0	0.09843520849767294	0.0
28	8.794092480435343E-5	0.0	0.0	0.16972598487240212	0.0
29	8.794092480435343E-5	0.0	0.0	0.25963092366405277	0.0
30	8.794092480435343E-5	0.0	0.0	0.42009379779039635	0.0
31	8.794092480435343E-5	0.0	0.0	0.6209215564019381	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9275	0.0	26.907278	1
CGCGTTA	45	0.0038273295	20.555555	35
GTATTGG	1575	0.0	20.320635	1
ATTGGAC	1550	0.0	19.812904	3
TTGGACC	2330	0.0	17.38841	4
TATTGGA	1870	0.0	16.917112	2
TATACCG	200	0.0	16.650002	5
GTATCAA	15115	0.0	16.547802	2
GTATCGT	80	3.385007E-4	16.1875	6
TGGACCC	2385	0.0	15.8239	5
CGTCTGT	855	0.0	15.578947	34
GGACCCT	2470	0.0	15.504049	6
ACCGTGT	195	4.1836756E-11	15.179487	8
GCGTTAT	165	1.6483682E-8	14.575757	1
TATACTG	550	0.0	14.463636	5
TGGACTG	520	0.0	14.230769	5
TAGACTG	360	0.0	13.875	5
TCGTAGA	95	0.0012459528	13.631579	2
TAGAACG	110	2.4585825E-4	13.454545	4
GACCCTC	2850	0.0	13.242105	7
>>END_MODULE