FastQCFastQC Report
Wed 25 May 2016
SRR2088147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1143218
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108680.9506498323154464No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT108040.9450515999573134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81690.7145618770873097No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42970.37586881942026806No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG35400.3096522273092271No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA27520.24072399139971556No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT19160.16759708122160427No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT19030.16645994027385852No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA15820.1383813061025981No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG14420.12613517281918235No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA13770.12044946808045359No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13640.11931232713270785No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT13540.11843760332674957No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT13370.11695057285662051No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.10872816908061281No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA12370.10820333479703782No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTCG250.005496331529.618
GGTATCA22850.027.1225381
CGAACTA450.003825796320.55555524
CGTGACT500.007034859618.512
TATATCG701.21918994E-418.55
CCGTATC500.007034859618.514
GTACTAG1850.018.01
ACGGACC953.6063539E-617.5263168
ATTGGAC4500.017.2666663
GTATTGG4350.017.0114941
GTATCAA37200.016.6102142
CGAGCCG803.3825444E-416.187515
TGCGATC700.002592482115.85714334
CAATGCG957.059623E-515.57894819
CCGAATT957.059623E-515.57894814
CGACGAG1001.0933555E-414.824
TGCGACG1001.0933555E-414.822
GACGAGT1152.2097123E-514.47826125
CGAATTA908.273968E-414.38888815
ACTGATC1551.2098644E-714.3225818
Produced by FastQC (version 0.11.5)