##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1143218
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.56245965336445	31.0	31.0	34.0	30.0	34.0
2	31.74812502952193	31.0	31.0	34.0	30.0	34.0
3	31.83867556319092	31.0	31.0	34.0	30.0	34.0
4	35.56086940548522	37.0	35.0	37.0	33.0	37.0
5	35.426118203177346	37.0	35.0	37.0	33.0	37.0
6	35.45174236234909	37.0	35.0	37.0	33.0	37.0
7	35.33661471390408	37.0	35.0	37.0	33.0	37.0
8	35.34770708648744	37.0	35.0	37.0	33.0	37.0
9	36.89834047399533	39.0	37.0	39.0	33.0	39.0
10	36.739271075158015	39.0	35.0	39.0	32.0	39.0
11	36.81377042698768	39.0	37.0	39.0	32.0	39.0
12	36.7045043027664	39.0	35.0	39.0	32.0	39.0
13	36.73927982239608	39.0	35.0	39.0	32.0	39.0
14	37.76376771534388	40.0	37.0	41.0	32.0	41.0
15	37.7825917716481	40.0	37.0	41.0	32.0	41.0
16	37.66548549795402	39.0	36.0	41.0	32.0	41.0
17	37.66253068093749	39.0	37.0	41.0	32.0	41.0
18	37.63981147952534	39.0	36.0	41.0	32.0	41.0
19	37.672875164666756	39.0	37.0	41.0	32.0	41.0
20	37.59587322802825	39.0	37.0	41.0	32.0	41.0
21	37.51801493678371	39.0	36.0	41.0	32.0	41.0
22	37.432549172598755	39.0	36.0	41.0	32.0	41.0
23	37.24273848032484	39.0	36.0	41.0	31.0	41.0
24	37.106427645470944	39.0	36.0	41.0	31.0	41.0
25	37.01125507121127	39.0	36.0	40.0	31.0	41.0
26	37.120110075243744	39.0	36.0	41.0	31.0	41.0
27	37.023624540551324	39.0	36.0	41.0	31.0	41.0
28	36.94593332155372	39.0	36.0	41.0	31.0	41.0
29	36.84418807261607	39.0	36.0	40.0	30.0	41.0
30	36.68664856571537	39.0	35.0	40.0	30.0	41.0
31	36.55222363538713	39.0	35.0	40.0	30.0	41.0
32	36.413033209764016	38.0	35.0	40.0	30.0	41.0
33	36.255553184082125	38.0	35.0	40.0	30.0	41.0
34	36.13673245172836	38.0	35.0	40.0	30.0	41.0
35	35.96458680671578	38.0	35.0	40.0	29.0	41.0
36	35.86887802676305	38.0	35.0	40.0	28.0	41.0
37	35.742362349088275	38.0	35.0	40.0	27.0	41.0
38	35.65331896453695	38.0	35.0	40.0	27.0	41.0
39	35.583858021829606	38.0	35.0	40.0	26.0	41.0
40	35.43786399444375	38.0	34.0	40.0	26.0	41.0
41	35.32372565862329	38.0	34.0	40.0	25.0	41.0
42	35.174795183420834	38.0	34.0	40.0	25.0	41.0
43	34.84063669396388	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	2.0
14	6.0
15	9.0
16	15.0
17	55.0
18	85.0
19	165.0
20	395.0
21	809.0
22	1347.0
23	2309.0
24	3836.0
25	5981.0
26	8887.0
27	12637.0
28	17481.0
29	23463.0
30	29905.0
31	37732.0
32	47078.0
33	58530.0
34	72900.0
35	90208.0
36	115387.0
37	155550.0
38	213721.0
39	244719.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.80780568535485	23.25671919091547	13.819324048431708	23.116151075297974
2	17.825996441623555	23.184467004543315	35.917559030736044	23.071977523097082
3	19.15111553526974	26.172873415219144	32.14408800421267	22.531923045298445
4	13.373214907392992	16.619402423684722	37.0379052814074	32.96947738751489
5	11.645110556341836	40.07004788238114	35.433749293660526	12.851092267616501
6	31.16317272821107	38.11302831131071	15.715637787368639	15.008161173109592
7	24.686280306993066	34.08037662108189	23.017657174747075	18.215685897177966
8	26.38158251532079	35.27883570762532	20.690629433756293	17.6489523432976
9	25.027072701794406	14.274267899910603	22.652285040998304	38.046374357296685
10	15.959510784469805	28.225587770661413	34.53890684016522	21.275994604703566
11	32.3238437463371	22.96359924353885	23.91757302631694	20.794983983807114
12	18.979932086443704	27.956435255568053	32.285793260777915	20.777839397210332
13	29.480641487450338	21.779835516935528	27.90036545960613	20.839157536008006
14	20.217928689016443	22.688586078945573	28.342100981615054	28.75138425042293
15	22.757426842474487	29.951680256958863	25.542897330168003	21.747995570398647
16	21.7426597551823	28.63705784898418	26.819906614486477	22.80037578134704
17	19.817130241126364	27.959146899366527	28.425724577464667	23.797998282042446
18	20.71398455937538	27.16227351213854	30.808559697275584	21.315182231210493
19	20.576565449459334	26.05863448616099	31.786675857098125	21.57812420728155
20	22.925898647502052	25.80548941671667	30.850721384722775	20.417890551058505
21	22.608111488797412	26.20506325127841	30.148668058060668	21.038157201863513
22	21.57733695585619	25.82543311949252	30.972307993750974	21.624921930900317
23	21.01663899623694	26.97971865383505	31.433987218535748	20.56965513139226
24	20.564231843795323	26.56737385170632	30.569060319204212	22.299333985294144
25	21.050140918005138	26.901868235104764	30.93740651389324	21.110584332996858
26	21.727789450481012	26.745904980502406	30.304281423140644	21.222024145875938
27	21.065361112228814	27.397836633083106	30.134410060023548	21.402392194664536
28	21.096151390198546	26.968084827215804	30.37006065334871	21.565703129236944
29	20.47229837178911	26.813258713561194	30.956038130960152	21.75840478368955
30	20.225888675650662	27.06491675253539	31.572630941780133	21.136563630033816
31	20.877295493947788	26.620557059108584	31.371269521648536	21.13087792529509
32	21.093264801638885	26.818157166874556	30.31451569167036	21.774062339816204
33	21.681778978287607	26.623706064810037	30.31836447641657	21.376150480485787
34	20.913771476656244	27.74300264691424	30.330523137319393	21.012702739110125
35	20.466262777527998	28.239845768698537	30.371372739057644	20.922518714715828
36	20.903712152887728	26.545593228937964	31.10885237986106	21.441842238313253
37	20.83985731505277	26.715727009196844	30.840924478096042	21.60349119765434
38	20.8686357282688	26.381320098179	31.43757358614018	21.312470587412026
39	21.196307265980767	26.215384992188717	31.47544912693817	21.112858614892346
40	20.56781821139975	26.435902863670798	30.99583806413125	22.000440860798204
41	20.92479299661132	26.217309384561826	30.807335083947248	22.05056253487961
42	19.761497807067418	27.25473181842833	30.818531548663508	22.165238825840742
43	20.159234721636643	26.915951288380697	30.512203271816922	22.41261071816574
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	123.0
1	163.5
2	204.0
3	396.0
4	588.0
5	588.0
6	1016.0
7	1444.0
8	1560.0
9	1676.0
10	2692.0
11	3708.0
12	3708.0
13	7080.5
14	10453.0
15	16113.0
16	21773.0
17	21028.5
18	20284.0
19	20284.0
20	23319.5
21	26355.0
22	22961.5
23	19568.0
24	22600.0
25	25632.0
26	25632.0
27	29025.0
28	32418.0
29	35462.0
30	38506.0
31	41785.5
32	45065.0
33	45065.0
34	48963.5
35	52862.0
36	57095.0
37	61328.0
38	63966.0
39	66604.0
40	66604.0
41	69026.0
42	71448.0
43	74825.5
44	78203.0
45	86473.0
46	94743.0
47	94743.0
48	96681.5
49	98620.0
50	90565.0
51	82510.0
52	77880.5
53	73251.0
54	73251.0
55	65502.5
56	57754.0
57	50775.5
58	43797.0
59	38948.0
60	34099.0
61	34099.0
62	29931.5
63	25764.0
64	22234.0
65	18704.0
66	15749.5
67	12795.0
68	12795.0
69	10803.5
70	8812.0
71	7320.0
72	5828.0
73	4740.5
74	3653.0
75	3653.0
76	2824.0
77	1995.0
78	1537.5
79	1080.0
80	867.5
81	655.0
82	655.0
83	509.5
84	364.0
85	280.0
86	196.0
87	177.5
88	159.0
89	159.0
90	144.0
91	129.0
92	81.5
93	34.0
94	28.5
95	23.0
96	23.0
97	13.5
98	4.0
99	5.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1143218.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.62035186175131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.95433540186944	46.64617290589948
2	11.248999972764821	12.963426730470799
3	3.409806376720704	5.8942272962127085
4	1.4182525904773111	3.2688085316857176
5	0.7266072352651811	2.093368228063702
6	0.435901578763055	1.5070081407252076
7	0.2979610777938837	1.201803550051316
8	0.21625543327629293	0.9968571325916384
9	0.16852540762957557	0.8739443956735091
>10	0.9950474031966602	10.896869043972076
>50	0.08322096794722489	3.2566018645469126
>100	0.03929841832201837	4.134527081140918
>500	0.002741748619231195	1.039599589585888
>1k	0.0025894292514961285	2.607731786051153
>5k	1.523193677350664E-4	0.7169682606439105
>10k+	3.046387354701328E-4	1.90208546268513
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10868	0.9506498323154464	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10804	0.9450515999573134	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8169	0.7145618770873097	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4297	0.37586881942026806	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3540	0.3096522273092271	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2752	0.24072399139971556	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1916	0.16759708122160427	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1903	0.16645994027385852	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1582	0.1383813061025981	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1442	0.12613517281918235	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1377	0.12044946808045359	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1364	0.11931232713270785	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1354	0.11843760332674957	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1337	0.11695057285662051	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1243	0.10872816908061281	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1237	0.10820333479703782	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	8.747238059582687E-5	0.0	0.0	0.0	0.0
7	8.747238059582687E-5	0.0	0.0	0.0	0.0
8	8.747238059582687E-5	0.0	0.0	0.0	0.0
9	8.747238059582687E-5	0.0	0.0	0.0	0.0
10	8.747238059582687E-5	0.0	0.0	2.624171417874806E-4	0.0
11	8.747238059582687E-5	0.0	0.0	3.498895223833075E-4	0.0
12	8.747238059582687E-5	0.0	0.0	7.872514253624418E-4	0.0
13	8.747238059582687E-5	0.0	0.0	8.747238059582687E-4	0.0
14	8.747238059582687E-5	0.0	0.0	0.0011371409477457493	0.0
15	8.747238059582687E-5	0.0	0.0	0.0017494476119165373	0.0
16	8.747238059582687E-5	0.0	0.0	0.0023617542760873255	0.0
17	8.747238059582687E-5	0.0	0.0	0.0034114228432372477	0.0
18	8.747238059582687E-5	0.0	0.0	0.0039362571268122095	0.0
19	8.747238059582687E-5	0.0	0.0	0.004548563790982997	0.0
20	8.747238059582687E-5	0.0	0.0	0.006298011402899534	0.0
21	8.747238059582687E-5	0.0	0.0	0.008309876156603552	0.0
22	8.747238059582687E-5	0.0	0.0	0.014083053275928126	0.0
23	8.747238059582687E-5	0.0	0.0	0.021255788484785928	0.0
24	8.747238059582687E-5	0.0	0.0	0.030790277969731057	0.0
25	8.747238059582687E-5	0.0	0.0	0.0361260931860765	0.0
26	8.747238059582687E-5	0.0	0.0	0.04242410458897603	0.0
27	8.747238059582687E-5	0.0	0.0	0.07452646826764449	0.0
28	8.747238059582687E-5	0.0	0.0	0.14336723179656025	0.0
29	8.747238059582687E-5	0.0	0.0	0.22086776100446284	0.0
30	8.747238059582687E-5	0.0	0.0	0.3590741223458693	0.0
31	8.747238059582687E-5	0.0	0.0	0.5306949330748816	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTTCG	25	0.0054963315	29.6	18
GGTATCA	2285	0.0	27.122538	1
CGAACTA	45	0.0038257963	20.555555	24
CGTGACT	50	0.0070348596	18.5	12
TATATCG	70	1.21918994E-4	18.5	5
CCGTATC	50	0.0070348596	18.5	14
GTACTAG	185	0.0	18.0	1
ACGGACC	95	3.6063539E-6	17.526316	8
ATTGGAC	450	0.0	17.266666	3
GTATTGG	435	0.0	17.011494	1
GTATCAA	3720	0.0	16.610214	2
CGAGCCG	80	3.3825444E-4	16.1875	15
TGCGATC	70	0.0025924821	15.857143	34
CAATGCG	95	7.059623E-5	15.578948	19
CCGAATT	95	7.059623E-5	15.578948	14
CGACGAG	100	1.0933555E-4	14.8	24
TGCGACG	100	1.0933555E-4	14.8	22
GACGAGT	115	2.2097123E-5	14.478261	25
CGAATTA	90	8.273968E-4	14.388888	15
ACTGATC	155	1.2098644E-7	14.322581	8
>>END_MODULE