FastQCFastQC Report
Wed 25 May 2016
SRR2088146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1528387
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT158211.0351435860158453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT157251.028862454339117No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT129160.8450739243398432No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG92380.6044280669751836No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63410.41488183293890885No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT48390.31660829358009457No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA47490.3107197326331616No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT44870.2935774774320902No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA39140.2560869727366171No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA37060.2424778541037054No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG36440.2384212898958183No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT32690.2138856192835977No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT32300.2113339095399267No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27780.18176024789533016No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26080.1706374105511235No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21930.14348460174026606No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT21880.14315745946543643No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19080.12483749207497839No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC18530.1212389270518527No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17560.1148923669201583No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG17020.11135923035199854No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT16030.10488181331037232No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15900.10403124339581533No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC15530.10161039056207624No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15390.10069439219255333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATA303.6000286E-430.83333435
TTCGCGT303.6000286E-430.83333418
GGTATCA36300.028.336091
ATTCGCG358.8707387E-426.42857217
TCGCGTC450.003826377220.55555519
ATTGGAC6450.018.9302333
GTATTGG6400.018.7890621
CATACCG500.00703591918.4999985
AACCGTG852.7240672E-517.4117647
GTATCAA62000.016.5604842
TAGGGGT904.4475004E-516.4444454
TTGGACC10050.016.3830834
ATCTGCG1708.54925E-1116.3235336
GGACCCT10500.016.2095246
TACCGTC803.3834783E-416.18757
GTACTAG1151.2427736E-616.0869561
TTACTCG700.002593017915.85714319
TATTGGA7750.015.754842
TAGACTA1302.5903864E-715.6538465
TTAGAAC1201.936418E-615.4166673
Produced by FastQC (version 0.11.5)